... (Color View II; Soft Imaging SystemGmbH, Münster, Germany) and were further analyzed byAnalysis® software (Soft Imaging System).ImmunohistochemistryImmunohistochemistry was performed, as described ... thin slices (3 to 4 mm) with a saw and fixed in 4% buff-ered formalin. After decalcification with EDTA, sections 4 to 6µm thick were prepared and stained with hematoxylin and eosin for the evaluation ... Involvement of the zygapophy-seal joint in ankylosing spondylitis: relation to the bridgingsyndesmophyte. J Rheumatol 1999, 26:1738-1745.6. Simkin PA, Downey DJ, Kilcoyne RF: Apophyseal arthritis...