... BL21(DE3)/pNA101; lane 3, recombinant NaoA after80% ammonium sulfate fraction; lane 4, recombinant NaoA afterSephadex G75 chromatography; lane 5, purified recombinant NaoAafter DEAE-Sepharose Fast ... DEAE-Sepharose Fast Flow chromatography; lane 6, standardmolecular mass markers (phosphorylase b,97kDa;BSA,66kDa;ovalbumin, 45 kDa).Fig. 3. Assay of NaoA activity. (A) Protein extracts from BL21(DE3)/pET23b. ... 31891–31895.39. Fiala, E.S., Conaway, C.C. & Mathis, J.E. (1989) Oxidative DNA and RNA damage in the livers of Sprague–Dawley rats treatedwith the hepatocarcinogen 2-nitropropane. Cancer Res. 49,...