Báo cáo y học: " Binding site of ABC transporter homology models confirmed by ABCB1 crystal structure" docx

... recognition site in the ABCB1 model, which is confirmed by both the ABCB1 X-ray crystal structure and the site- directed mutagenesis studies. The ABCB1, ABCC4 and ABCC5 models display distinct ... ATP -binding cassette (ABC) transporters ABCB1, ABCC4 and ABCC5 are involved in resistance to chemotherapeutic agents. Here we present molecular models of ABCB1, ABCC4 and AB...

Ngày tải lên: 13/08/2014, 16:20

... 5-day cultures of 50 mL to 7-day cultures of 500 mL resulted in a even lower expression when analyzed by SDS/ PAGE. Therefore, we focused on the purification and study of the rBla g ... candidate for clinical use in diagnosis of cockroach allergy and develop- ment of new forms of immunotherapy. Keywords: allergen; hypersensitivity; IgE antibody; asthma; cockroach. Sensitization ....

Ngày tải lên: 08/03/2014, 23:20

... proliferation of synoviocytes in the synovial lining layer, gross infiltration of lymphocytes, and migration of atypical lymphocytes with nuclear indentation into the synovial fluid (SF) and/or synovial ... study indicates that HTLV-I infection could modify the inflammatory activity of ordinary primary OA, whereby direct activation by Tax protein might account for the increased conc...

Ngày tải lên: 09/08/2014, 01:23

... to bind to Tyr757 of gp130, Tyr985 of the leptin receptor and Tyr401 of the erythropoietin receptor, Tyr729 of the granulocyte colony-stimulating factor (G-CSF) receptor, Tyr800 of the IL-12 ... forms of cytokine receptors that lack intracellular domains can inhibit the action of cytokines by simple competition for cytokine binding. Endocytosis of receptors and proteasomal...

Ngày tải lên: 09/08/2014, 06:23

... acid substitutions were chemically synthesized by a solid-phase procedure described previously, using a model 430 peptide synthesizer (Applied Biosystems, Foster City, CA, USA) and purified by high-performance ... liquid chromatography [14]. Measurement of T-cell cytokines by ELISA Quantitative measurement of cytokines was performed as described previously [12]. In brief, spleens an...

Ngày tải lên: 09/08/2014, 08:22

... and type I collagen α1 chain were synthesized by Invitrogen (Table 1). Data analysis was carried out using the Gene Amp 5700 Sequence Detector System software (Applied Biosystem, Foster City, CA, ... result of biomechanical forces as well as biological sources, such as cycles of hypoxia, the pres- ence of reactive oxygen species, accumulation of advanced glycation end products and t...

Ngày tải lên: 09/08/2014, 10:20

... days did not modify the membrane expression of RANK-L as evaluated by cytofluorometry and did not stimulate the release of detectable amounts of OPG in neutrophil super- natants as measured by ... without any significant changes from day 1 to day 3 (Figure 3). The expression of OPG mRNA by neutrophils incubated in CM was not yet detectable on day 2 and appeared only after 3 days...

Ngày tải lên: 09/08/2014, 10:20

... cartilage, key compo- nents of the skeletal system. Its metabolism is characterized by two opposing processes: formation and resorption. Typically, diseases of bone are characterized by an alteration ... Framingham Heart Study cohort [25]. As part of a study of the inheritance of OA, selected participants in the Offspring cohort were originally examined between 1992 and 1994. Me...

Ngày tải lên: 09/08/2014, 13:21

... presence of rheumatoid factor of any of the immunoglobulin isotypes tested (IgM, IgA, and IgG) was considered as positive. The study was approved by the Ethical Committee of the University of Gothenburg. ... signifi- cantly lower than that of plasma (94 ± 24 versus 141 ± 32 mg/L, P = 0.0001) as detected by its ability to promote actin filament assembly. In vitro, pGSN can be c...

Ngày tải lên: 09/08/2014, 13:22

... active immune system by blocking deposition of host immune factors such as complement on pathogen surfaces, thereby inhibiting detection by innate immunity path- ways. In a complement deposition assay, BtE264 ... presence of this cluster was confirmed by whole genome sequencing of a representative variant strain (B. thailandensis E555). Both whole- genome microarray and multi-lo...

Ngày tải lên: 09/08/2014, 20:22