... complementa-tion assays. The ataP5, ataP4 and ataP10 genes were alsoindependently inserted in the pIJ702 vector and the resultingplasmids pA 2A5 , pA 2A4 and pA 2A1 0, respectively(Materials and methods), ... betweenard1 and ard2 are part of the ata cluster, ata12 and ataPKS1should also pertain to it. Further sequencing of the atacluster could provide an insight into the biosyntheticpathway of the other ... similarities suggest that certain enzymes, and therefore the corresponding genes of the A2 0 1A biosyn-thetic pathway, may be related to their counterparts of the puromycin and hygromycin A biosynthetic...