Báo cáo y học: "Early gene expression changes with rush immunotherapy" ppt

... study to evaluate gene expression changes in patients undergoing IT. The goal of the study was to iden- tify a unique gene profile in RIT patients c ompared to healthy controls and those with ... allergic patients to healthy controls, the authors found only modest differences in the gene expressions of the 32 genes. More recently, Zhang et al. analyzed gene expression of chemo...

Ngày tải lên: 13/08/2014, 13:22

... is hypothesised to be due to increased OB biosynthetic activity [18]. The lack of differential gene expression of these anabolic growth factors in our study is most likely due to the gene expression ... non-linearly associated with OCN/GAPDH mRNA gene expression. The data in this study also indicated that with increasing ALP gene expression there is decreasing bone vol- ume...

Ngày tải lên: 09/08/2014, 08:23

... markedly stronger with lymphoma cDNA than with cDNA of B- lymphocytes were further analyzed by Northern blot hybridization. Some genes (a-myb, set, SMG-1, ND4) involved in HIV-associated lymphomagenesis ... of lymphomas h1 and h2 were analyzed by dot blot hybridization with [ 32 P]-labeled cDNA populations from SIV-associated monkey lymphomas and monkey B-lymphocytes. Those cDNAs...

Ngày tải lên: 02/11/2012, 11:08

... analyzed individually. Flow cytometry analysis The cell type composition and level of protein expression for some genes in the inguinal lymph nodes were analyzed by flow cytometry. Single cell suspensions ... pre-annealed with a poly dT primer, mixed with reverse transcriptase, deoxy- nucleotides, RNase inhibitors and dUTP-Cy3 or dUTP- Cy5. After labelling, the RNA was hydrolyzed and th...

Ngày tải lên: 09/08/2014, 01:23

... human primary subchondral osteoblasts [39]. In the present study, we used microarray analysis to survey comprehensively the expression levels of many thousands of genes simultaneously in trabecular ... 3 Differentially expressed genes in OA bone with roles in osteogenesis, angiogenesis and chondrogenesis Real-time PCR Rank GenBank Role Cell type Pathway Symbol Name t OA/CTL t OA/OP Symbol...

Ngày tải lên: 09/08/2014, 10:21

... classification of the genes was performed using gene ontology and pathway analysis. Quantitative RT-PCR analysis of host genes using SYBR Green I The differential expression data was validated by quanti- tative ... selectively taken for RT-PCR analysis. The expressions of these genes were found to be matching with the microarray analysis. Chakrabarti et al. Virology Journal 2010, 7:219...

Ngày tải lên: 12/08/2014, 01:21

... pneumonectomy. Experimental time points were at Day0,3,6,14and21days.Day0studiedgene expression in the right lung without any prior surgery (sham thoracotomy or left pneumonectomy)(N = 15 mice). Days ... used for gene expression analysis. Angiogenesis gene expression in the CD11b - CD31 - cells was compared on Day 0 and Day 14 after pneumonectomy (N = 3 mice, each time point). Gene...

Ngày tải lên: 12/08/2014, 13:22

... histology of generalized pulmonary emphy- sema. I. The genesis of the early centrolobular lesion: focal emphysema. Australas Ann Med 1957, 6:124-140. 8. McLean KH, Gough J: The histology of generalized ... downregulation of decorin gene expression in COPD patients, at least partially via the Smad pathway. Our findings may explain why only a sub- set of smokers demonstrates an excess paren...

Ngày tải lên: 12/08/2014, 14:20

... microarray analysis was performed as described in the Affymetrix GeneChip Expression Analysis Technical Manual using Affymetrix Mouse Genome 430 2.0 GeneChips and the One-Cycle cDNA Synthesis ... further investigation. Microarray data analysis to identify temporal changes in lung regeneration (time dependent) In a second analysis, the focus was shifted to temporal changes in gene ex...

Ngày tải lên: 12/08/2014, 14:20

... expression of the cytokine genes. Bhsp65-restimulated LNC revealed changes in multiple cytokine genes (12 out of 61 genes; 19.7 %) that showed a high level of expression in contrast to only ... microarray analyses can be further extended to comparative analysis of homologous genes of interest. However, neither the early phase events are easy to study in RA patients nor the microarra...

Ngày tải lên: 12/08/2014, 18:20