Báo cáo y học: "Two-dimensional difference gel electrophoresis (DIGE) analysis of sera from visceral leishmaniasis patients" ppt

... study examining the global pro- teome profile of the visceral leishmaniasis patients’ sera. Here, we report, for the first time, the difference- in -gel elect rophoresis (DIGE) analysis of sera from ... Access Two-dimensional difference gel electrophoresis (DIGE) analysis of sera from visceral leishmaniasis patients Lokesh A Rukmangadachar 1,2 , Jite...

Ngày tải lên: 13/08/2014, 13:20

... encoding the secreted CM from Yersinia pestis (*YpCM, y2 828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the protein. Results ... and 20% poly(ethylene glycol) 400. The crystallization drops were made with equal volumes of protein and well solution. Crystallization of *YpCM Crystallization conditions were...

Ngày tải lên: 17/03/2014, 17:20

... inhibition of the caseinolytic activity in zymography by EDTA and lack of inhibition by the serine protease inhibitor phenylmethylsulfonyl fluoride further confirmed the MMP character of the bands ... weeks of co-culture when samples were analysed by laser scanning micoscopy (LSM) (but not by histology), showing that LSM is a suitable and sensitive tool for the analysis of initial...

Ngày tải lên: 09/08/2014, 01:22

... major secretory product of articular chondrocytes and synovial cells). It is of note that none of these 27 genes were identified by differential expression analysis. Pathway analysis software was ... http://arthritis-research.com/content/ supplementary/ar1018-s1.xls Interestingly, literature searches and pathway analysis revealed that nearly all of the 19 genes identified by thi...

Ngày tải lên: 09/08/2014, 01:23

... evaluated by a blinded examiner (SSL, I-MJ, MB) for synovitis and erosion of bone/cartilage. Synovial hypertrophy (synovitis) was defined as a membrane thickness of more than two cell layers. A ... smoke delay onset of arthritis? A first line of evidence in support of a direct influence of smoking on the development of CIA comes from the collagen II antibody levels. At the end...

Ngày tải lên: 09/08/2014, 14:21

... methylation analysis of CpG sites. Biotechniques 2003, 35:146-150. 11. Tost J, Dunker J, Gut IG: Analysis and quantification of multiple methylation variable positions in CpG islands by pyrose- quencing. ... methylation data from a different source into a format that can be read by MethMarker. On completion of data import, MethMarker displays a high- resolution DNA methylation pro...

Ngày tải lên: 09/08/2014, 20:20

... products were denatured and a nalyzed by electrophoresis on 5% denaturing polyacrylamide gels, and results were analyzed using GeneScan Analysis soft- ware (Applied Biosystems, CA, USA). Tumor samples that ... status of DNA ploidy using flow cytometry by following the method of Dressler et al.[24].TheDNAindex(DI), representing the ratio of the mean fluorescence intensity of the G...

Ngày tải lên: 10/08/2014, 05:21

... recent analysis showed that life expectancy at age 20 years had increased by over 13 years since the introduction of combination antiretro- viral therapy and currently life expectancy of HIV ... levels of accuracy in this type of analysis are likely to be dependent on the time periods between VL measure- ments, which are typically every six months in patients with HIV. The limitat...

Ngày tải lên: 10/08/2014, 05:21

... Prodromal Syndromes: preliminary evidence of interrater reliability and predictive validity. American Journal of Psychiatry 2002, 159:863-865. 36. Yung AR, Yuen HP, Mcgorry PD, Phillips LJ, Kelly D, ... British Journal of Psychiatry Suppl 1973, 122:15-30. 6. Gottesman II, Gould TD: The endophenotype concept in psychiatry: etymology and strategic intentions. American Journal of Psychia...

Ngày tải lên: 11/08/2014, 15:22

... consent was obtained from the patient for publication of this case report and accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal. References 1. ... presented case is a rare, usually benign, but quite frightening manifestation of a food allergy which is typi- cally a Type I allergic reaction developing within seconds or m...

Ngày tải lên: 11/08/2014, 23:21