Báo cáo y học: "Liver sinusoidal endothelial cells represents an important blood clearance system in pigs" doc

... Hepatology Open Access Research Liver sinusoidal endothelial cells represents an important blood clearance system in pigs Geir I Nedredal* 1 , Kjetil H Elvevold 2 , Lars M Ytrebø 1 , Randi Olsen 3 , ... centrifugation, and centrifugal elutriation. This method yields both high purity and functionally intact pig liver sinusoidal cells that can be cultivated in monolaye...

Ngày tải lên: 13/08/2014, 13:20

... Zhang G, Liu D: Hydrodynamics-based transfec- tion: simple and efficient method for introducing and expressing transgenes in animals by intravenous injection of DNA. Methods Enzymol 2002, 346:92-105. 31. ... thereby. Background Following partial hepatectomy (PHx) the remaining liver is transfused by normal blood volume, thereby exposing liver sinusoidal endothelial cells (LECs) to e...

Ngày tải lên: 13/08/2014, 13:20

... morphology (Figure 7h and inset). In contrast, CD163 + cells were fewer in number (Figure 7f) and most were located in the synovial lining (type A synoviocytes), with only scattered cells in the subintimal ... first 6 days, PMN cells and typical inflammatory mono- cytes did not increase until days 9 to 12 and days 12 to 14, respectively. The delayed appearance of MHC II - cells...

Ngày tải lên: 09/08/2014, 10:22

... for injury to skin and peritoneum [53]. Another elegant study in an IC-induced inflammation model argued for a codominant role of inflammatory pathways mediated by FcγRI/III and C5aR [54]. In ... cells, macrophages, granulocytes and particular neutrophils into the synovial lining and fluid and the periarticular spaces. These infiltrating cells produce abundant cytokines, dominated ma...

Ngày tải lên: 09/08/2014, 06:23

... diamide. In both cases, changes in mRNA levels were well-correlated with changes in PolII occupancy, and in general PolII changes typically explain approximately 50% of the variance in mRNA changes. ... changes and changes in mRNA abundance, but there was signifi- cant variation as well - changes in PolII abundance typi- cally accounted for approximately 50% of variance in mRN...

Ngày tải lên: 09/08/2014, 20:22

... mapping pro- cesses can be difficult if the mutant phenotype is subtle and if assaying the phenotype is labor intensive. The circadian clock is an endogenous 24-h timer found in most eukaryotes and ... photosynthetic bacteria. In plants, the clock plays a key role driving rhythms in physiology, biochemistry and metabolism [7]. In Arabi- dopsis, our current model of the clock is a se...

Ngày tải lên: 09/08/2014, 22:24

... together with our findings is that CO may have an anti- inflammatory effect in mice but not in humans or pigs. The cytokine levels following endotoxin infusion in our study were high, and individual ... that administration of CO had any effect on cytokine release during endotoxin-induced inflammatory response. Pro-inflammatory cytokines (TNF-alpha, IL-6 and IL-1beta) were neither atte...

Ngày tải lên: 11/08/2014, 08:22

... Desmosine and Isodesmosine (D/I) are cross-linking amino acids which are present only in mature elastin. Changes in their concentration in body fluids indicate changes in elastin degradation and can ... demon- strated in lung parenchyma. Recently, techniques for detecting and quantifying elastin degradation in body flu- ids have advanced in specificity, sensitivity and accuracy...

Ngày tải lên: 12/08/2014, 14:20

... linked to preterm birth, inherited genetic diseases, and inflammation and may increase the risk of hypoxic injury to vital organs in new- born infants; such injuries representing ~4% of infant fatalities ... siRNA-generating pDNAs invoked an inter- feron and/or cytokine response. Since both ENaC and CFTR can be affected by cytokines and interferons [35,36], this could potentially explain...

Ngày tải lên: 12/08/2014, 15:21

... clearance of pathogens during infection by killing and phagocyting pathogens. In the present study, using a model of LPS-induced lung inflammation we propose yet another mechanism by which they ... the clearance and resolution of an inflammation by removing pro-inflammatory cell debris from the tissue, thereby act- ing as a back up system stepping in when the macrophage system i...

Ngày tải lên: 12/08/2014, 16:20