Báo cáo y học: "HIV-1 Tat protein enhances Microtubule polymerization" docx

... by the human immunodeficiency virus type-1 Tat protein. Virology 1999, 257:502-510. 15. Flores SC, Marecki JC, Harper KP, Bose SK, Nelson SK, McCord JM: Tat protein of human immunodeficiency ... Thus, it seemed Tat would be able to act quantitatively and qualitatively on tubulin polymerization. In order to address these Retrovirology 2005, 2:5 http://www.retrovirology.com/content/2/1...

Ngày tải lên: 13/08/2014, 13:20

... respect, the systematic study of protein protein interaction through the isolation of protein complexes is under way, and cell- map proteomics adds a route to efficiently study the genome at the protein ... More recently, methods were devised for predicting residues involved in protein interaction s ites in the absence of any structural reports. By analysing hydrophobicity distribution...

Ngày tải lên: 21/02/2014, 15:20

... oligomycin of L6 cells showed that they were very well coupled. The oligomycin resistant respiratory activity of the cells could be strongly stimulated by the addition of the protonophore carbonyl ... interacting proteins u sing a yeast-two hybrid system revealed that the C-terminals of UCP2 and UCP3 (but not that of UCP1) specifically interact with members of the 14.3.3 protein family [37]....

Ngày tải lên: 21/02/2014, 15:20

... activity and inverse agonism in a family C G -protein- coupled receptor. J. Biol. Chem. 275, 29547–29555. 52. Chen, R., Mukhin, Y. V., Garnovskaya, M.N., Thielen, T.E., Iijima, Y. , Huang, C., Raymond, ... Department of Medicinal Chemistry, The Royal Danish School of Pharmacy, Copenhagen, Denmark; 2 Laboratory of Molecular Cardiology, Copenhagen University Hospital, University of Copenhagen...

Ngày tải lên: 08/03/2014, 09:20

... resistance and enzyme catalysis. Biochem. Pharmacol. 53, 1019–1027. 20. Tacke, R., Chen, Y. & Manley, J.L. (1997) Sequence-specific RNA binding by an SR protein requires RS domain phospho- rylation: ... min and analysed using 7.5% polyacrylamide/7 M urea gels. Gels were exposed for 24 h using Rentgen XS-1 (Foton) or Biomax MS (Kodak) films. Mobility shift assay The mobility shift assay w...

Ngày tải lên: 24/03/2014, 03:21

... factor; Stat3. Biosynthesis o f the neurotransmitter acetylcholine (ACh) from choline and acetyl coenzyme A is catalyzed by the enzyme choline acetyltransferase (ChAT, acetyl-CoA:cho- line O-acetylotransfe ... ngámL )1 CNTFfor20min.Celllysates(500lgper sample) were immunoprecipitated with a Stat3 antibody and then analyzed by Western blotting using an antibody speci®c for phos- phoTyr705 Stat...

Ngày tải lên: 31/03/2014, 21:21

... of MN mutations still remains unresolved, these findings led us to the possibility that maintenance of MN integrity is partly regulated by DNA-PK, and changes in the phosphorylation status in ... by X-ray, UV-C, and 3-methylcholanthrene, detected by a DNA fingerprint assay. Cancer Res. 52, 5788–5793. 12. Kitazawa, T., Kominami, R., Tanaka, R., Wakabayashi, K. & Nagao, M. (1994) 2-Hydroxyami...

Ngày tải lên: 31/03/2014, 23:20

... YSYTATYHIYDLTNGEFIR 1098.96 (2+) 403–421 VTQDSLFYSSNEFEGYPGR The 97 kDa band obtained following biotinylated fluorophosphonate labelling of bovine chondrocytes was trypsin digested and analysed ... trypsin and ana- lysed by tandem electrospray mass spectrometry using a Q- Tof instrument (Waters, Manchester, UK) interfaced to a Waters CapLC capillary chromatography system as previously descri...

Ngày tải lên: 09/08/2014, 07:20

... metalloproteinases, CIA = collagen-induced arthritis; DMSO = dimethylsulfoxide; FLS = fibroblast-like synoviocytes; FPP = farnesylpyrophosphate; GGPP = geranylger- anylpyrophosphate; HMG = hydroxymethylglutaryl; ... viability in the presence of statins. Geranylgeranylpyrophosphate restores the viability of RA synovial fibroblasts in the presence of statins The decrease in cellular viability...

Ngày tải lên: 09/08/2014, 08:22

... fibroblast- like synoviocyte; GADPH = glyceraldehyde-3-phosphate dehydrogenase; HPRT = hypoxanthine phosphoribosyltransferase; IgG = immunoglobulin G; MMP = matrix metalloproteinase; OA = osteoarthritis; ... in the lining layer of the synovium from both patient groups, com- pletely lacking Thy-1 surface expression. This distinct synovial cell population is clearly characterised by cell surfa...

Ngày tải lên: 09/08/2014, 08:23