... with LightCycler software (version 4.05). The RNAs of reference strains were also tested with this assay in order to examine the assay specificity [6-8]. Detection of the mimic samples by the duplex ... compared with the single system. In order to improve the sensitivity of the duplex system, we further optimized the concentrations of the primers and probes f...
Ngày tải lên: 12/08/2014, 01:22
... management. Finally, there is a useful role for what is termed ‘just -in- time training’. The intent of these programs is to make concise knowledge available to providers, at the time of an event and at the ... are the solutions? We offer the following suggestions in order of priority. These concepts attempt to build on existing resources. We do not pretend to offer...
Ngày tải lên: 12/08/2014, 20:21
Báo cáo y học: "Base relative quantification framework and software for management and automated analysis of real-time quantitative PCR data" potx
... through the analysis by means of a simple workflow scheme in the main screen of the qBase Experiment Analyzer (Figure 4d). Step 1: Initialization The first step in the workflow is the (automatic) initialization of ... parts: the bottom of the screen pro- vides an explorer-like window to browse through the data; and the top of the screen contains a separat...
Ngày tải lên: 14/08/2014, 17:22
Báo cáo y học: " The connection domain in reverse transcriptase facilitates the in vivo annealing of tRNALys3 to HIV-1 genomic RNA" potx
... similarly in vivo is not known. Alternatively, the RT connection domain may undergo interactions with Gag that may result in placing the tRNA Lys3 bound to the thumb domain in RT closer to either ... viral packaging. tRNA Lys3 incorpora- tion into HIV-1 is not affected by deletion of the IN domain in GagPol, nor by further deletion of the RNaseH tRNA...
Ngày tải lên: 13/08/2014, 13:20
Báo cáo y học: "Placenta Percreta-Induced Uterine Rupture Diagnosed By Laparoscopy in the First Trimester"
... such as in the case of our patient, laparoscopy can be helpful for the early di- agnosis of hemoperitoneum due to uterine rupture. In general, the area of placenta percreta-induced uterine rupture ... collection in the peritoneal cavity. In addi- tion, we recommend laparoscopy for the investigation of acute abdomen with unclear diagnosis in the first trimest...
Ngày tải lên: 25/10/2012, 10:56
Báo cáo Y học: Cold induces stress-activated protein kinase-mediated response in the fission yeast Schizosaccharomyces pombe pot
... level only after 60 min (Fig. 1). Pyp1p and Pyp2p phosphatases appear to play a key role in the dephosphorylation of the osmotic stress-activated Sty1p [20]. Interestingly, however, the Sty1p activation ... DISCUSSION The main aim of this study was to analyse the cold shock response in S. pombe,ayeastthatdisplaysacascadeof stress activated protein kinases homologous to...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo Y học: Calcium-binding by p26olf, an S100-like protein in the frog olfactory epithelium pot
... constants of the first to fourth binding of Ca 21 by assuming the presence of four steps of sequential and ordered binding. To identify the site of the EF-hand corresponding to the nth binding of Ca 21 , ... 90% of the change in the a helix content was attained by binding of two Ca 21 . Interestingly, in the mutants which have only three Ca 21 -binding...
Ngày tải lên: 24/03/2014, 04:21
Báo cáo Y học: Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells pptx
... the PTB domain and two of the N-terminal proline-rich domains. This interac- tion was only slightly inhibited by phosphotyrosine. To test if the Shb PTB domain could be involved in this interaction we ... vector. The Shb DPTB-Tyr plasmid was constructed by the deletion of the nucleotides 880–1603 in the Shb cDNA (containing the four tyrosine phosphory- lation sit...
Ngày tải lên: 24/03/2014, 04:21
Báo cáo y học: "Collaboration between general hospitals and community health services in the care of suicide attempters in Norway: a longitudinal study" ppt
... 9:26 http://www.annals-general-psychiatry.com/content/9/1/26 Page 6 of 8 Predictors of a chain of care structure in the CHS To examine whether quality of care indicators at EDs in 1999 could predict whether or not the CHS ... the level of single quality indicators and in the multi- variate analyses. This study highlights several factors that could potentially contrib...
Ngày tải lên: 08/08/2014, 23:21
Báo cáo y học: "Nifedipine decreases sVCAM-1 concentrations and oxidative stress in systemic sclerosis but does not affect the concentrations of vascular endothelial growth factor or its soluble receptor" potx
... endothelium injury in SSc and that VEGF signalling may by impaired in this disease without implication of the sVEGFR-1. Endothelium injury is critical in SSc and is suspected to occur early in the ... cutaneous according to the criteria of LeRoy and colleagues [18]. The exclusion criteria were the impossibility of stopping vasodilator therapy, pregnancy, current ci...
Ngày tải lên: 09/08/2014, 01:23