Báo cáo y học: " Apoptosis of uninfected cells induced by HIV envelope glycoproteins" potx

... dysregulation of cytokine/ chemokine production [15]. However, HIV- 1 -induced apoptosis in bystander uninfected immune cells is likely the key to the depletion of T lymphocytes observed in HIV- 1-infected ... differentiation of HIV- specific CD8+ T cells may be compromised by Fas apoptosis induced by FasL- expressing HIV- infected cells [128]. In addition to dire...

Ngày tải lên: 13/08/2014, 13:20

... rate of processing by HIV- 1 IN is also relatively slow. Joining activity is confirmed by polyacrylamide gel electrophoresis To verify that joining has indeed taken place in the context of this ... necessary, the sensitiv- ity of the assay could be increased further by use of other reporters such as radioactivity or digoxygenin plus anti- body for amplification. Finally, the a...

Ngày tải lên: 10/08/2014, 05:21

... increase may be in response to the OVA -induced airway inflammation, which produces an "asthmatic" phenotype in Guinea pigs characterized by non-specific airway hyper-reactivity and airway eosi- nophilia ... might be a result of DR-independ- ent Bud effect. Table 1 data shows that "net" apoptosis by day 4 is approximately the same but timing to onset this apoptos...

Ngày tải lên: 12/08/2014, 16:20

... 14 days after cell transfer, coinciding with the main influx of polymorphonuclear cells. By day 14, CD45 + MHC II hi cells constituted approximately half of all CD45 + cells in SRT. Most of the ... mono- cytes did not increase until days 9 to 12 and days 12 to 14, respectively. The delayed appearance of MHC II - cells (mainly PMN cells) , which comprised approximately 92% o...

Ngày tải lên: 09/08/2014, 10:22

... immunotherapy with DC+AK cells and TIL. (b) Cytotoxic activity of autologous and allogeneic effector cells. Cytotoxic activity of TIL was examined by the 51 Cr-releasing test. Tumor cells obtained from ... aseptically and infused them intravenously back into the patient after removal of tumor cells by centrifugation, and then concentration by apheresis. After the drainage...

Ngày tải lên: 11/08/2014, 19:21

... Germany) followed by analysis of Dahal et al. Respiratory Research 2011, 12:60 http://respiratory-research.com/content/12/1/60 Page 2 of 11 the vessels using a computerized morphometric analysis system ... Vajnerova O, Bansova A, Vizek M, Herget J: Prevention of mast cell degranulation by disodium cromoglycate delayed the regression of hypoxic pulmonary hypertension in rats. Respi...

Ngày tải lên: 12/08/2014, 13:22

... that γδ intra-epithelial lymphocytes protect the lung by regulating the inflammatory response evoked by epithelial necrosis [35]. γγδδ T cells negatively regulate airway responsiveness to methacholine Mice ... expression of Vγ4 increased steadily so that, by 2–3 months of age, Vγ4 + cells seemed to be the pre- dominant population of pulmonary γδ T cells, at least in BALB/c mi...

Ngày tải lên: 12/08/2014, 18:20

... detection of cytolysis The Cytotoxicity Detection Kit (Roche Diagnostic GmbH, Panzberg, Germany) was used to quantify mam- mary leukocyte cytolysis under the procedure used pre- viously [28]. Statistical ... 52:12 http://www.actavetscand.com/content/52/1/12 Page 5 of 13 Figure 1 The flow cytometry analysis of th e cells from mammary glands. The representative dot plot (A) shows the di...

Ngày tải lên: 12/08/2014, 18:22

... approaches have helped to identify host proteins that physically interact with HIV- 1 IN or stimulate HIV- 1 IN catalytic activity. A recent study involving use of a biotinylated IN as a tool to detect ... the catalytic activity of PIC makes it pos- sible to identify physiologically relevant viral-host protein interactions. The integration activity of the isolated complex was confirme...

Ngày tải lên: 13/08/2014, 01:20

... T cells for 1–2 weeks before intracellular cytokine staining (ICCS) and flow cytometry (FACS) analysis. The d5 DCs were transduced by LV-PLAP and 48 hr later, analyzed by PLAP enzyme assay. ... modulation of DC immunity by LV expression of different cytokines or siRNA molecules. Materials and Methods Generation of monocyte-derived dendritic cells Peripheral blood mononuclear...

Ngày tải lên: 13/08/2014, 13:20