Báo cáo y học: "Lymphocytes, apoptosis and sepsis: making the jump from mice to humans" docx

... However, it is important to note that blockade of lymphocyte apoptosis is not always protective in sepsis. For example, septic mice lacking MyD88 have decreased lymphocyte apoptosis but a significant ... mortality [14]. MyD88 is an important proximal component of the main pathogen recognition pathways, suggesting that inhibition of lymphocyte apoptosis is protective only when the...

Ngày tải lên: 13/08/2014, 11:23

... shown these cytokines play a key role in the pathophysiology of COPD [37]. In vitro studies have shown that the pro-inflammatory cytokines GM- CSF, IL-8 and IL-6 inhibit apoptosis of granulocytes ... likely overwhelmed by the in vivo inhibition of the anti-apoptotic effects of inflamma- tory cytokines [7]. IL-6, IL-8 and GM-CSF are important cytokines in the regulation of airw...

Ngày tải lên: 12/08/2014, 14:20

... by patient age (18 to 64 and 65+ years old). The insurance category was recoded from the primary and secondary payers abstracted from the NIS record, which was prov ided by state-specific sources ... design and interpretation of the study and drafting, critical revision, reading and approval of the manuscript. SL contributed to the design, statistical analysis a...

Ngày tải lên: 14/08/2014, 08:21

... phosphatidylinositol synthase 1 (Eur. J. Biochem. 269) 2351 Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis Anne-Marie ... membrane and in addition to de novo synthesis, the plant enzyme is capable of catalysing the exchange of the inositol polar head for another inositol. Our data...

Ngày tải lên: 22/02/2014, 04:20

... homologous to regions adjacent to the beginning and the end of ybdQ and additional 20-nucleotide complementary to the template plasmid pKD13 carrying the kanamycin resistance (kan) gene. The resulted ... removed from GroEL by ATP [38]. Furthermore, GroEL seems to exhibit a high selectivity towards UP12 compared to other proteins of theUspAfamilyandalsoincomparisonwit...

Ngày tải lên: 22/02/2014, 07:20

... inhibitory activity. This fraction was further subjected to reversed- phase chromatography and the protein obtained was analysed by SDS/PAGE and MALDI-TOF spectrometry (Fig. 1). In SDS/PAGE, the inhibitor from ... Biosystems, Framingham MA, USA) tip, and a sample of the flow- through carrying the glycans was drop-dialysed and analysed by MALDI-TOF MS. RESULTS Isolation of c...

Ngày tải lên: 08/03/2014, 23:20

... factors [3] and their biotechnological potential. Lipases have proven to be versatile enzymes in nonaqueous solvent systems in which they catalyze the synthesis of a variety of acylglycerols and ... biocatalyst. Keywords: lipase; LipA; Acinetobacter sp.RAG-1;protein purification; zymogram. Lipases are glycerol ester hydrolases (EC 3.1.1.3) that catalyze the hydrolysis of triacylglyce...

Ngày tải lên: 23/03/2014, 21:20

... and amino-acid analysis. The sequence obtained, namely Lys- Ile-Glu-Glu-Gly-Lys-Leu-Val-Ile-Trp corresponds to the N-terminus of the m ature maltose-binding protein. Binding of the fusion protein to the IMAC ... contrast, the alkylmaltosides employed in this study are chemically well de®ned a nd commercially available at high purity. The combination of the cholesterol de...

Ngày tải lên: 24/03/2014, 00:21

... and H3 of the galactosyl residues are strongly coupled. Fig. 6. Methylation analysis and LSIMS analysis of A-7 and A-9. (A) GLC chromatogram of partially methylated alditol acetates derived from ... are considered to be the former type, and A-7 to be the latter. We presume that these two types of SEGLx present their unique carbohydrate chain differently on the cell mem-...

Ngày tải lên: 24/03/2014, 03:21

... acetone (twice), and ether, and dried. LPS was extracted from dry bacteria by the phenol/chloroform/petroleum ether method [10] in a yield of 4.4% of dry bacteria. De-O-acylated LPS was prepared after hydrazine ... efinitely identify the two differently phosphorylated LPS species bu t also to learn more about the recognition of charged carbohydrate epitopes b y antibodies. W e...

Ngày tải lên: 31/03/2014, 21:21