Báo cáo y học: " Functional analysis of human T lymphotropic virus type 2 Tax proteins" pptx

... appeared to only reduce the activity of Tax 2B. However the introduction of an arginine instead of a cysteine at this position (Y1 44R) into Tax 2B abolished its activity indicating that this position is ... prevalence of mutations in Tax 2A pro- teins which inactivate both pathways may influence the pathogenic properties of certain HTLV-2A viruses. Table 4: Transactivation ph...

Ngày tải lên: 13/08/2014, 09:21

... availability of an annotated transcription start site [28 ], the availability of human and chimpanzee DNA sequence of high quality, the possibility to place primers for amplification of the promoters ... 20 03) to identify the transcription start sites and to collect promoter sequences 2, 000 bp upstream and 1,000 bp downstream of the start sites. Out of a total of 71 genes...

Ngày tải lên: 14/08/2014, 14:21

... speculate that DM64 binds to the myotoxins through a myotoxic/ cytolytic site distinct from the catalytic site, as already described for the inhibition of myotoxicity by heparin [44]. At least in the ... intracerebroventricular lethality and its anticoagulant effect, activities that depend on the enzymatic activity of this protein [44]. These results confirm that the myotoxicity induced by...

Ngày tải lên: 21/02/2014, 01:21

... possibility was not investigated systematically, but preliminary data suggest detergent does not affect the ability of p26 to form large complexes in bacterial extracts. Published results vary in terms ... p26-alpha, demonstrating that enhanced thermotolerance conferred by these two constructs correlated with synthesis of p26 polypeptides. Additionally, when tested by centrifugation on su...

Ngày tải lên: 22/02/2014, 04:20

... by PCR from the TriplEx clone by using Pfu DNA polymerase (Stratagene) and the upstream primer (5¢-TTTTTT CTCGAGAAGCTTTCC CTGCAGGAT-3¢) and downstream primer (5¢-TTTTT T GGATCCCTACAGATGTGCTTCAAGGCC-3¢), ... broad speci®city w ith respect to the g lycone moiety of substrates. Human CBG hydrolyses synthetic aryl glycosides (including 4-nitrophenyl and 4-methylumbelliferyl monoglycosides)...

Ngày tải lên: 08/03/2014, 16:20

... considered to be statistically significant. RESULTS Determination of the transcriptional start site of the Bsep gene The transcription initiation site of the Bsep gene was located by both 5¢ RACE ... in the p- 126 minimal promoter construct completely abolished the stimulation of luciferase activity by CDCA in HepG2 cells (Fig. 6). Interestingly CDCA reduced the activity of the mutan...

Ngày tải lên: 17/03/2014, 23:20

... opposed to strong adhesion or none at all) favors cell motility [ 32] , we investigated the motility of the arthritogenic SF clone by studying its ability to migrate to fibronectin. The arthritic SF ... Incidence Derived Transgene Clone genotype of arthritis Synovium hTNF/TAg B2 wt 31/65 (47.6%) Synovium hTNF/TAg B1 wt 5/8 ( 62. 5%) Synovium hTNF/TAg A4 wt 4/8 (50.0%) Synovium TAg F6 wt 0...

Ngày tải lên: 09/08/2014, 01:21

... post-treatment. We previously reported that this dose of V 2 O 5 causes minimal cytotoxicity (<10% by lactate dehydrogenase assay) and yet induces H 2 O 2 pro- duction, activates intracellular ... validated by RT-PCR. CXCL9 and CXCL10 are STAT1-dependent chemokines that function in the recruitment of lymphocytes [28 ]. We previously showed that V 2 O 5 activates STAT1 in lun...

Ngày tải lên: 12/08/2014, 15:20

... indicates that othernetworks contain this gene product. Ingenuity pathway analysis of a network of genes expressed in EB and BC data sets associated with GATA2Figure 6 Ingenuity pathway analysis of ... 1.48E- 02 Gas transport (erythrocytic) 5 2. 00E- 02 Oxygen transport (erythrocytic) 5 2. 00E- 02 Neurogenesis (neuronal) 51 2. 02E- 02 Cell homeostasis 15 2. 31E- 02 Percept...

Ngày tải lên: 14/08/2014, 08:20

... the type of Th cell that stimulates them. This is consistent with the capacity of fibroblasts to activate flexible patterns of cytokine production readily. A corollary of this observation is the ... NP40 to detect cytokine content. Statistical analysis Student's t test was used with two-tailed P values or Mann– Whitney U test using StatView 5.0™ (SAS Institute Inc., Cary, N...

Ngày tải lên: 09/08/2014, 07:20