Báo cáo y học: " Coordinate enhancement of transgene transcription and translation in a lentiviral vector" pps

... vector transgene mRNA. In addition, we reasoned that coordinate enhancement of transgene transcription and translation has significant potential for synergistically improving efficiency of transgene ... the availability of mRNA available for translation enhancement by PCE. We project that the activity of PCE and CMV IE to co-ordinately stimulate pro- tein outpu...

Ngày tải lên: 13/08/2014, 09:21

... line immunoblot assays (LIAs) for single-parameter or profile anal- ysis – have been established in clinical laboratory practice dur- ing past years, allowing examination of a large number of sera in a rapid ... performed using alkaline phos- phatase-labelled goat anti-human IgG. Control sera were included in each assay. Incubated blot strips were digitalized using a flatbed s...

Ngày tải lên: 09/08/2014, 01:22

... completely removed safely and reliably by cutting the ascending aorta, main pulmonary artery and superior vena cava. Although preoperative examination could not determine whether the tumor was lipoma ... mediastinal lipoma causing clin- ical symptoms is considered clinically malignant. We achieved complete removal of a 15 × 10 cm large medi- astinal lipoma by cutting the ascending a...

Ngày tải lên: 10/08/2014, 09:22

... nausea, diarrhea and abdominal pain subsided within a few days. Nine days after admission, her intestinal gas disappeared. However, her serum amylase and lipase lev- els were elevated in the absence ... copy of the written consent is available for review by the Editor -in- Chief of this journal. Abbreviations Alb: albumin; ALT: alanine aminotransferase; ALP: alkaline phosphatase;...

Ngày tải lên: 11/08/2014, 12:20

... arteria mammaria interna and a compar- atively thin RCA. To lessen the risk of a relapse, the implantation of an automatic implantable cardioverter defibrillator (AICD) was indicated. The athlete ... long-distance runner collapsed at the finish of a half marathon because of a myocardial infarction with ventricular fibrillation. Coronary angiography showed an anomalous origin...

Ngày tải lên: 11/08/2014, 23:21

... genotype A is prevalent in Europe, India, Africa and Ame rica. Genotypes B and C are predomi- nant in China, Japan and Southern Asia whereas geno- type D is widespread in the Mediterranean area and ... drafted the manuscript. VT participated in the sequence alignment, in phylogenetic analysis and drafted the manuscript. JG and AF participated in the sequence analysis...

Ngày tải lên: 12/08/2014, 02:20

... lung transplantation affecting both short- and long-term morbidity and mortality of patients [1,2]. Surfactant alterations in both the intra-alveolar and intracellular surfactant system have been ... primary graft dysfunc- tion and known to inactivate the intra-alveolar surfac- tant [11,12], which can be compensated by the prophylactic intratracheal administration of exogenous s...

Ngày tải lên: 12/08/2014, 13:22

... statistical analyses and helped draft the manuscript. DR: Performed and co-ordinated most laboratory analy- ses and helped draft the manuscript. HK: Co-ordinated laboratory analyses, participated in ... a monoclonal antibody against CD68 (IgG1 isotype, Dako, Glostrup, Danmark) as a marker for macrophages and rabbit polyclonal antiserum against iNOS (Transduction Laboratories, Le...

Ngày tải lên: 12/08/2014, 18:21

... 5'-CCCTAG- GAAAAAGGGCTGTTG-3'/RT 3' OUTER 5'-GTTTTCA- GATTTTTAAATGGCTCTTG-3', for the first round of amplification, and PR 5' INNER 5'-TGAAAGATTGTACT- GAGAGACAGG-3'/RT ... technical guidance; Dr. Luis Padilla-Noriega and Dr. Eduardo Garc a- Zepeda for their academic coun- selling. We thank Dr. Indiana Torres, Dr. Beatriz Ramírez, Dr. Adakatia Ar...

Ngày tải lên: 12/08/2014, 23:21

... Chechnya: quantitative assessment of living conditions, and psychosocial and general health status among war displaced in Chechnya and Ingushetia Kaz de Jong* 1 , Saskia van der Kam 1 , Nathan ... giving a reason. Forms were registered anonymously and data were ana- lysed by EXCEL and EPIINFO-6 using descriptive and uni- variate analyses. Instruments The survey questionna...

Ngày tải lên: 13/08/2014, 13:21