Báo cáo y học: " Targeted infection of HIV-1 Env expressing cells by HIV(CD4/CXCR4) vectors reveals a potential new rationale for HIV-1 mediated down-modulation of CD4" doc

... and (-) CAAGTTAACAGCACTATTC and the fusion primers (+) GGGATATTGATGTCTGTAGAATAGGAGCTTTGTTCCTT- GGG and (-) CCCAAGGAACAAAGCTCCTATTCTACAGT- CATCAATATCCC produced a 1457 bp fragment with a 1448 bp ... RNA polymerase promoter. After DNA transfection, the func- tionality of the CD4 proteins was initially evaluated in HeLa cells by a syncytia-forming assay. Infection of trans- fecte...

Ngày tải lên: 13/08/2014, 09:21

... remaining sites ATCAGC GGTAATGA CATAGGGAT ATCAGC GGTAATGA CATAGGGAA ATCAGC GGTAATGA CATAGGGA T ATCAGC GGTAATGA CATAGGGAA GSS GSS GSS GSS SNP A- genome A- genome B-genome ATCAGC GGTA TGA CATAGGGATA ATCAGC ... generate new phenotypic variation that eventually can play a critical role in the origin of new adaptations and important agronomic traits. Background Comparative analysis of...

Ngày tải lên: 09/08/2014, 23:20

... exclu- sion. Plasma and cell-associated viral loads Viral load was assayed as previously described [72,78]. Briefly, viral RNA load in EDTA-anticoagulated, cell-free plasma was determined by real-time RT-PCR after ... Cafaro A, Cranage M, Ensoli B, Hunsmann G, Polyanskaya N, Stahl-Hennig C, Thortensson R, Titti F, Heeney J: Comparison of early plasma RNA loads in different macaque specie...

Ngày tải lên: 12/08/2014, 23:21

... Scientist. Author details 1 Ottawa Hospital Research Institute, 501 Smyth Rd., Ottawa, Canada. 2 Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 450 Smyth Rd., Ottawa, Canada. 3 Division ... In the first round of PCR, DNA (1/10) was amplified with outer P24 primers (400 nm) forward (fwd): 5’-ATAGAGGAAGAGCAAAA- CAAAA-3’ ; reverse (rvs): 5’ -GTTCCTGAAGGGTAC-...

Ngày tải lên: 13/08/2014, 01:21

... reasons for this dis- crepancy are not completely clear. One simple explana- tion would be that virus growth in spreading assays is generally measured by p24 gag ELISA or by enzymatic RT assays ... cannot be accurately measured. Alternatively, a difference in the rate rather than absolute amount of HIV-1 DNA nuclear import may not be detectable in spreading assays due to the high...

Ngày tải lên: 13/08/2014, 09:20

... 2001;413(6853):316-22. 4. Yada M, Hatakeyama S, Kamura T, Nishiyama M, Tsunematsu R, Imaki H, Ishida N, Okumura F, Nakayama K, Nakayama KI. Phosphorylation-dependent degradation of c-Myc is mediated by the F-box ... Nakayama K, Oike Y, Nishiyama M, Ishida N, Hatakeyama S, Bessho Y, Kageyama R, Suda T, Nakayama KI. Mouse Fbw7/Sel-10/Cdc4 is required for notch degradation durin...

Ngày tải lên: 31/10/2012, 16:49

... allophycocyanins and phycocyanins, HPLC separation allowed a quantitative estimation of the relative amount of each protein component from the area underlying each peak. This was facilitated by the fact ... phycobilisomes before and after illumination reve aled the d isappearance of the b-phycocyanin already after 1 h of illumination (Fig. 4). In contrast, SDS/PAGE analysis of t...

Ngày tải lên: 08/03/2014, 16:20

... dislocation of the radial head and distal radio-ulnar joint. A case report D -A. J. Verettas, G.I. Drosos, K.C. Xarchas, C.N. Chatzipapas  , C. Staikos Department of Orthopaedic Surgery, Medical ... dislocations, K-wire stabilization of the DRUJ and immobilization of the forearm with a long arm cast in supination. Under general anaesthesia firstly the radial head was redu...

Ngày tải lên: 08/08/2014, 17:20

... treatment of condylar process fractures has generated a great deal of discussion and controversy in oral and maxillofacial trauma and there are many different methods to treat this injury. For ... Cranio-Maxillofacial Surgery 2006; 34: 306-14. 16. Terai H, Shimahara M. Closed treatment of condylar fractures by intermaxillary fixation with thermoforming plates. British Journal...

Ngày tải lên: 08/08/2014, 17:20

... responsibilityFamily FinancialHeavy workloadNil Annals of General Psychiatry 2009, 8:8 http://www.annals-general-psychiatry.com/content/8/1/8 Page 4 of 5 (page number not for citation purposes) Almost ... in collection and analysis of data and wrote the initial draft of the arti- cle. SA took part in data analysis and manuscript writing. ES: did literature search and reviewed the ma...

Ngày tải lên: 08/08/2014, 23:21