Báo cáo y học: " DBR1 siRNA inhibition of HIV-1 replication" pptx

... effect of DBR1 overexpression on HIV-1 replication in GHOST- R5X4 cells. In contrast, the three DBR1 siRNA& apos;s DBR1 siRNA inhibition of HIV-1 replicationFigure 3 DBR1 siRNA inhibition of HIV-1 ... DBR1 siRNA pHyper-D123 (black bar, DBR1 siRNA) , control vector pHyper (white bar, DBR1 siRNA) , or cotransfected CDM9 -DBR1 and DBR1 siRNA pHyper-D1...

Ngày tải lên: 13/08/2014, 09:21

... only a target of destruction but also plays an important role in perpetuating and aug- menting tissue injury and inflammation via induction of cytokines, chemokines and modulatory enzymes. Hyaluronan ... additional 18 h. Promoter activity was assayed by luciferase activity and results are displayed as fold induction of luciferase activity. These data represent the average of 6 ident...

Ngày tải lên: 11/08/2014, 08:22

... tis- sue. Hydroxyproline measurement The total collagen content of the right lung was deter- mined by hydroxyproline measurement. The hydroxypro- line content was determined after acid hydrolysis of ... central role of HSP47 in the progression of ble- omycin-induced pulmonary fibrosis. In fact, HSP47 pro- tein may actively participate in the pathophysiology of pulmonary fibrosis. Co...

Ngày tải lên: 12/08/2014, 15:20

... light microscopy; some assays were stained using Giemsa. A syncytium formation value of 100% is defined as the number of syncytia formed in the absence of peptide but in the presence of 1.5% DMSO. ... inhibitory activity, as reduction of P BLV -391 and subsequent modification of the cysteine res- idues with the sulfhydryl reactive agent Iodoacetyl-PEO 2 - Biotin failed to disrupt...

Ngày tải lên: 13/08/2014, 05:21

... from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proceedings of the National Academy of Sciences USA 1980, 77:7415–7419. 4. Yoshida M, Miyoshi I, Hinuma Y: Isolation and ... a rapamycin-sensitive regulator of c-myb expression. CMAT (c -myb in activated T cells) binds to a region of the c-myb promoter thereby enhancing expression. Concentrations of...

Ngày tải lên: 13/08/2014, 09:20

... inflammatory response when subjected to hyperchloremic acidosis whereas hyperlactemia attenuated this response [9]. Hyper- chloremia also caused hypotension in a cecal ligation and puncture model of ... of hyperchloremia, especially when modest, are unlikely to influence outcome for most patients. However, given that hyperchloremic acidosis is often iatrogenic, and associated with morbidi...

Ngày tải lên: 12/08/2014, 23:24

... density under an optical microscope (×100). A, control; B, siRNA1 ; C, siRNA2 ; D, siRNA3 . (B) The quantification of TLR4-EGFP fluorescence intensity upon siRNA knockdown was evaluated by flow cytometry ... cytometry analysis. Immunofluorescence and flow cytometry results revealed that all 3 siRNAs had efficient inhibition on GFP fluorescence, and TLR4 -siRNA2 was the most poten...

Ngày tải lên: 25/10/2012, 11:48

... main metabolite of aceclofenac is 4-hydroxy- aceclofenac. The other metabolites, namely diclofenac and 4- hydroxy-diclofenac, account for only 5% of the administered dose [25]. Aceclofenac acts as ... healthy cartilage of proteoglycan synthesis rate as a measure of cartilage matrix synthesis (pg synthesis); percentage release of newly formed proteoglycans (new pg release) as a me...

Ngày tải lên: 09/08/2014, 07:20

... diseases. Gouty inflammation is due to monosodium urate (MSU) crys- tal-induced release of proinflammatory cytokines from leuko- cytes. Among the many cytokines implicated [2,3], IL-1 may have a special ... involving 10 patients with gouty arthritis refractory to conventional therapies, anakinra given at 100 mg daily for 3 days rapidly relieved the inflammatory symptoms of gout. These res...

Ngày tải lên: 09/08/2014, 10:20

... significant downregulation of synthesis of collagen type II. In contrast, pre-treatment of chondrocytes with the phytochemical agents followed by stimulation with IL-1β resulted in an inhibition of cytokine-induced ... manner. Finally, inhibition of NF-κB activation by resveratrol occurred mainly through the accumulation of phosphorylated IκBα, ubiquitinated IκBα and inhibiti...

Ngày tải lên: 09/08/2014, 14:22