Báo cáo y học: " Identification of unique reciprocal and non reciprocal cross packaging relationships between HIV-1, HIV-2 and SIV reveals an efficient SIV/HIV-2 lentiviral vector system with highly favourable features for in vivo testing and clinical usa

... cord injury and other inflammatory or destructive conditions of the CNS[24,25]. We investigated the cross packaging ability of the Gag-Pol components of HIV-1, HIV-2 and SIV and found a unique non -reciprocal ... 60% of glial cell expressing GFP with 20 ng of input vector and approximately 58% with 10 ng of vector. In summary, the gene transfer effi...

Ngày tải lên: 13/08/2014, 09:21

... 7a) and between SI+ and I+ in the NSm gene (Figure 7b). Relative Synonymous Codon Usage (RSCU) was ana- lyzed within and among I+ and SI+ isolates using the GCUA (General Codon Usage Analysis) ... SI+ genotype arises and, because they survive and are maintained at a higher rate in stably-infected mosquito lineages than I+ genotype, SI+ maintain an excess of intermediate-f...

Ngày tải lên: 12/08/2014, 04:20

... according to Form-1 in Figure 1. This form developed by us for this study. The presence of dyspeptic com- plaint and its severity calculated by presence of ab - dominal pain, abdominal pain with ... were minimally 12 and maximally 60. The patients investigated previous smoking history, coronary artery disease, diabetes mellitus and malignancy history in their family. S...

Ngày tải lên: 25/10/2012, 11:18

... enables identification of protein- protein interactions between a soluble bait and its interacting prey [5]. However, such a screening system is not suitable for the analysis of interaction between ... 3.1 Principle of Split-Ubiquitin Screening System Studies have shown that the use of yeast two hybrid system, which is a genetic assay for in vivo detection of...

Ngày tải lên: 02/11/2012, 11:08

... difference between isotype and antibody staining. A maximum of 2% positive cells by staining with isotype anti- body mouse IG1 or IG2 conjugated with FITC, PE or biotin was allowed and therefore was ... supplemented with antibiotic solution (100 units/ml penicillin, 100 µg/ml streptomycin; Bio- chrom, Berlin, Germany); fine mincing and digestion with 0.2% pronase (Roche, Man...

Ngày tải lên: 09/08/2014, 01:23

... the anti-SmD3 peptide (SMP) assay. (a) Intra-assay and interassay variability, (b) linearity, and (c) receiver operating characteristic analysis. The intra-assay and inte- rassay variability, ... the type I and type II protein arginine methyltransferases, which preferentially methylate arginines located in RG clusters. Recently, however, using arginine methyl-specific antibod- ies...

Ngày tải lên: 09/08/2014, 06:22

... link integrins to the cytoskeleton and recruit sig- naling molecules [33]. A new study reported that a kinectin isoform lacking a major portion of the kinesin-binding domain is very probably ... splicing in the 3'-untranslated region. (b) Autoradiography of in vitro transcription and translation products of the candidate clones BD44 and BD42 labeled by [ 35 S]-methionine...

Ngày tải lên: 09/08/2014, 07:20

... identify undeiminated and deiminated proteins reacting with RA and non- RA serum samples. Proteins from HL60 lysates incubated (+) or without (-) peptidylarginine deiminase (PAD) blotted with an anti- body ... were counterstained for 1 minute with haematoxylin, washed in tap water, dehydrated, cleared and mounted. Figure 1 Screen to identify undeiminated and deiminated...

Ngày tải lên: 09/08/2014, 07:20

... Ltd., for kind anal- ysis with microarrays, and Dr. Sumio Sugano, Human Genome Center, Institute of Medical Science, University of Tokyo, for kindly providing a mouse spleen cDNA library. We ... was used for the analysis. Sample labeling and processing were performed according to the manufacturer's protocol. In brief, double-stranded complementary DNA was synthesized,...

Ngày tải lên: 09/08/2014, 08:22

... the experiments and statistical analysis, and drafted the manu- script. KH and KT participated in the design of the study and its coordination. TN and NM conceived of the study, partici- pated in its ... in RANKL/RANK and its costimulatory pathways are similar between the two subsets, except for NFATc1, integrin αv and integrin β3. We therefore assumed that the dis...

Ngày tải lên: 09/08/2014, 08:22