Báo cáo y học: "Efficient trapping of HIV-1 envelope protein by hetero-oligomerization with an N-helix chimera" ppt

... MO, Wang S, Liu J, Fagan MB, Nunberg JH: Structural and functional analysis of interhelical interactions in the human immunodeficiency virus type 1 gp41 envelope glyco- protein by alanine-scanning ... transport of secre- tory proteins in cultured rat hepatocytes. J Biol Chem 1986, 261:11398-11403. 31. Wadia JS, Dowdy SF: Transmembrane delivery of protein and peptide drugs by T...

Ngày tải lên: 13/08/2014, 09:21

... Inhibition of HIV-1 replication by an improved hairpin ribozyme that includes an RNA decoy. RNA Biol 2005, 2:75-79. 16. Ikeda M, Habu Y, Miyano-Kurosaki N, Takaku H: Suppression of HIV-1 replication by ... Basu A, Pandey VN: Inhibition of HIV-1 replication by anti-trans-activation responsive polyamide nucleotide ana- log. Antiviral Res 2002, 56:13-27. 44. Tripathi S, Ch...

Ngày tải lên: 13/08/2014, 09:21

... Afp-Lu1 and 2.7a genes, followed within 30 bp by the ÔCAÕ dinucleo- tides necessary for primary transcript cleavage and poly(A) attachment [43]. AFP gene regulation by the hormonal regime preceding and ... diversity can also be generated by alternative polyadenylation [42] and several sites containing the canonical sequence AATAAA, or a single nucleotide variant of it, were found down...

Ngày tải lên: 21/02/2014, 15:20

... intensity of pol II signal in the 1 M eluate probably because of the limiting quantities of antigen in this fraction that occasionally might be below the sensitivity of our antibody. Importantly, ... Puri- fication of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibo...

Ngày tải lên: 17/03/2014, 10:20

... surrounding the lesion of lymphocytic infiltration, identified by morphol- ogy and by reactivity with anti-Mac-3 antibody (Fig. 4b, arrowheads). On the other hand, tissue sections stained with preimmune ... secretion: an early Th1-domi- nant stage represented by high IL-2 and IFN-γ expression fol- lowed later by a Th2-dominant stage represented by increased expression of I...

Ngày tải lên: 09/08/2014, 01:23

... clinician summary screen interview rates, and final DSM-IV diagnoses. Subjects were classified according to the presence/ absence of any affective/anxiety disorder, any disruptive disorder, and any ... interview) or one or more psychiatric disorders (for final diagnoses). d Any affective disorder (depressive disorders, dysthymia, mania, hypomania, bipolar disorder) and/or any anxiety disord...

Ngày tải lên: 11/08/2014, 16:22

... JA, Loftus SK, Zhang D, Coleman KG, Cooney AM, Comly ME, Fanding L, Roff C, Tagle DA, Pavan WJ, Pentchev PG, Rosenfeld MA: Substantial narrowing of the Niemann-Pick C candidate interval by yeast ... concentrating and sorting of proteins [1]. A variety of viruses like HIV-1 and measles virus exploit DRMs for their assembly and budding [6,23] after association of certain structural...

Ngày tải lên: 19/06/2014, 08:20

... JA, Loftus SK, Zhang D, Coleman KG, Cooney AM, Comly ME, Fanding L, Roff C, Tagle DA, Pavan WJ, Pentchev PG, Rosenfeld MA: Substantial narrowing of the Niemann-Pick C candidate interval by yeast ... cholera toxin or anti cav-1 antibody, respectively. A-MLV Env was detected using an anti-Env antibody (83A25 [21]). As expected for a DRM-associated protein and from the results of the D...

Ngày tải lên: 20/06/2014, 04:20

... supernatant was removed for analysis and cells analysed by flow cytometry. Serum was prepared by cardiac puncture. Sera and lavage supernatants were assayed without delay or storage. Flow Cytometry Phenotypic ... specificity of locus degradation is guided by the antisense strand of the unwound siRNA, followed by sense strand siRNA binding to the complementary mRNA site for cleavag...

Ngày tải lên: 11/08/2014, 08:21

... putative transmembrane domains of a membrane protein based on its hydropathy profile [5,6]. Sophisticated algorithms that combine hydropathy and sequence analysis can predict up to 95% of transmembrane helices ... Identification of novel membrane proteins by searching for patterns in hydropathy profiles John D. Clements and Rowena E. Martin School of Biochemistry and Molecular Biolog...

Ngày tải lên: 17/03/2014, 23:20