Báo cáo y học: " Inhibition of Tat activity by the HEXIM1 protein" docx

... reduction of P-TEFb activity, thus the inhibition of Tat activity is unlikely due to a global reduction of cellular P-TEFb activity. To further investigate the mechanism of inhibition of Tat- mediated ... rescued Tat function, ectopic expression of HEXIM1 effectively inhib- its Tat activity. Most importantly, Tat enhancement medi- ated by hCycT1 was eff...

Ngày tải lên: 13/08/2014, 09:21

... The KKLEB immortalized CF airway cell line homozygous for ΔF508 mutation and the CF 14 clinically isolated mucoid strain of PA (originally from the laboratory of Dr. M.C. Wolfgang, University ... http://www.journal-inflammation.com/content/6/1/15 Page 4 of 5 (page number not for citation purposes) fashion by ~70% in the KKLEB cells. Inflammation in CF is regulated largely by...

Ngày tải lên: 11/08/2014, 08:22

... after end of surgery the distribu- tion of the labeled neutrophils was analyzed by scintigra- phy (Figure 6). In Figure 6A an example for the total body distribution of radioactivity is provided. ... Abstract Background: The arterial in line application of the leukocyte inhibition module (LIM) in the cardiopulmonary bypass (CPB) limits overshooting leukocyte activity...

Ngày tải lên: 11/08/2014, 08:21

... effect of TNF-α on Sox9 activity. Finally, the sensitivity of Sox9 to atRA was reduced by caMEKK1 expression. Thus, the activity state of p300, the common co-factor and MEKK1 target, probably plays ... atRA. Regulation of transcription factor activity by p300 Differences in the affinity for p300 may contribute to the pat- tern of regulation of transcription fact...

Ngày tải lên: 09/08/2014, 10:22

... 30:1 http://www.jeccr.com/content/30/1/1 Page 7 of 7 and the effects of developing the HDV ribozyme as a tool of cancer gene therapy Methods The bel7402, HCT116 cells were given by Department of molecular Biology, Shandong University, DNA ... DNA of HDV ribozyme was synthesized by Shanghai Biosun Sci&Tech. Co. LTD. Recombinant plasmid pBBS212 containing hTR gene was pr...

Ngày tải lên: 10/08/2014, 10:20

... increased the turbidity plateau by about two- fold over the control (in the absence of Tat) . Electron microscopy data as well as the reversibility of the major part of the holo -Tat- induced turbidity ... conformations of the apo-form and zinc-bound form of Tat, and studied the interaction of the two forms of Tat with tubulin. The 86-aa-long Tat prot...

Ngày tải lên: 13/08/2014, 05:21

... and cells were lysed in 50 µl of cell lysis buffer (Promega, Pittsburgh, PA). 20 µl of the cell lysates were used in the firefly luciferase assay system of Promega as described by the manufacturer. ... infectivity, whereas the reduction of luciferase activities observed at high concentrations of the other HDPs is primarily due to modulation of cellular activity and/...

Ngày tải lên: 13/08/2014, 13:20

... have found that the TAT PTD and the TAR RNA binding domain are located in the same region of the TAT protein. The close proximity of these two properties led us to hypothesize that the sequence of this ... on TAT activated LTR-Luciferase activity and specific TAR RNA binding by Tat- PFigure 6 Inhibitory effect of Tat- P on TAT activated LTR-Luciferase activi...

Ngày tải lên: 13/08/2014, 09:21

... Such a hypo- thesis is further confirmed by the observation that, differ- ently from the effect of serum, the stimulatory effect of PMA on ERK1/2 activity could also be totally abolished by NAMI-A. ... integrity and equal loading. The levels of radioactivity incorporated into c-myc product were normalized by comparison with the levels of radioactivity incorporated int...

Ngày tải lên: 21/02/2014, 01:21

... 2). The sixfold stron ger splicing inhibition by Cl-tetracycline may be explained by the a dditional s ize and hydrophobicity introduced by the substitution, as is often the case in ribozyme inhibition ... of chlorine for hydrogen at position 7 (Fig. 1C). Inhibition by Cl-tetracycline w as found to be sixfold more t han by tetracycline (Fig. 2). The extent of spl...

Ngày tải lên: 21/02/2014, 15:20