Báo cáo y học: " Kinetic studies of HIV-1 and HIV-2 envelope glycoprotein-mediated fusion" potx

... The requirement of cofactor for TDP enzymatic activity was examined by determining the specific activity of TDP by following the cleavage of 1 m M of substrate by 0.125 l M of enzyme in reaction ... displayed standard Michaelis–Menten kinetics with a K m value of 211 l M and a V max of 1.103 l M Æmin )1 , and turnover number or rate constant of phosphodiester bond hydrolysi...

Ngày tải lên: 31/03/2014, 21:21

... serum, 60 mg/ml of penicillin and 100 mg/ml streptomycin. Sup-T1 cells Sequence comparison of HIV-1 and HIV-2 EnvsFigure 4 Sequence comparison of HIV-1 and HIV-2 Envs. The HIV-1 subtype B infectious ... this analysis by comparing only one HIV-1 and two HIV-2 strains we believe that the methodology developed here can be expanded to include comparisons between variou...

Ngày tải lên: 13/08/2014, 09:20

... monomeric and dimeric prototypes for the respective families of c-andb-crystallins, andsimplyreferredtoasc-type or b-type crystallins, respectively. Both monomeric c-type crystallin and the subunit of dimeric ... evolutionary pathway to c-type genes, but rather the acquisition of an interdomain intron in the evolution of c-crystallins, and of both interdomain and intermotif...

Ngày tải lên: 24/03/2014, 04:21

... reduction by NAD + at 2.1 m M dihydroxyacetone phosphate and varying NADH concentration; B-2, in hibitio n of dihydroxyacetone phosphate reduction by NAD + at 0.32 m M NADH and varying dihydroxyacetone ... purified by TLC chromatography [16] and its concentration was measured by the phosphate analysis [17]. Glyceraldehyde phosphate, dihydroxyacetone phosphate, sn-glycerol-2-phosphate,...

Ngày tải lên: 22/02/2014, 04:20

... poly(ethylene glycol) by the addition of 0.67 vol. of 30% poly(ethylene glycol) in buffer A, and after 10 min centrifuged at 10 000 g for 20 min. The resulting pellet was resuspended in 50 mL of ... control by metabolic intermediates of the pathway of sucrose synthesis; 6PF2K activity is stimulated by Fru-6-P and P i , and inhibited by three-carbon phosphate esters (including...

Ngày tải lên: 22/02/2014, 04:20

... chromatogram abundant in hydrophilic proteins such as linkers and a significant amount of a -and b-phycocyan ins, and only traces of the allophycocyanins. On the contrary, HPLC a nalysis of bands 2–5 revealed ... phycocyanin and allophycocyanin are composed of two polypeptide chains, a and b, of approximately 17 000 and 18 000 [7]. The a and b polypeptides contain one or...

Ngày tải lên: 08/03/2014, 16:20

... x-3 hydroxy fatty acids, respectively. 2080 T. Kitazume et al. (Eur. J. Biochem. 269) Ó FEBS 2002 Kinetic analysis of hydroxylation of saturated fatty acids by recombinant P450foxy produced by an Escherichia ... University of Tsukuba, Tsukuba, Ibaraki, Japan; 2 Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan Cytoch...

Ngày tải lên: 08/03/2014, 22:20

... pattern. A widely discussed possibility is that of * Correspondence: patten@ucalgary.ca 1 Department of Community Health Sciences & Department of Psychiatry, University of Calgary, 3330 Hospital ... interpretation may be premature and that the projected increase may not occur. Author details 1 Department of Community Health Sciences & Depart ment of Psychiatry, Universit...

Ngày tải lên: 11/08/2014, 16:22

... maximum of 1.4 mg/kg/day as fol- lows: 0.5 mg/kg/day for the f irst 10 days; 0.8 mg/kg/day over the next 10 days; and 1.2 mg/kg/day for a minimum of 10 days, with an increase to 1.4 mg/kg/day allowed thereafter. ... 5, 6, 8, 10, 12 in Study LYCS; 2) week 3 and months 1, 2, and 3 in Study S012; and 3) week 3 and m onths 1, 2, 4, and 6 in Study S013. Symptom improvement was oper...

Ngày tải lên: 13/08/2014, 18:21

... approxi- mately 30% of the original values, and becomes equal to TCA cycle and glyoxylate bypass reactions considered in E. coli and M. tuberculosis modelsFigure 1 TCA cycle and glyoxylate bypass reactions ... construction of a kinetic model of the TCA cycle and glyoxylate bypass in M. tuberculosis, and we study the likely metabolic conse- quences of inhibiting ICLs and...

Ngày tải lên: 13/08/2014, 23:20