Báo cáo y học: " The strength of the HIV-1 3'''''''' splice sites affects Rev function" doc

... splice sites and their strengthFigure 10 Schematic overview of HIV-1 splice sites and their strength. (A) Schematic drawing of the HIV-1 pre-mRNA and the distribution of the splice sites. The relative ... Neither the mutations of the upstream AGs (Fig. 4B, lane 2) nor of the branch sites (lane 3) led to splicing at the 3'ss A5 but efficiently...

Ngày tải lên: 13/08/2014, 09:20

... over the progression of cancer; and the second question is whether the PR domain alone possesses anticancer activity. In this study, we quan- titatively analyzed the mRNA expression level of ... research grant from the Cancer Research Society of Canada. W. Sun would also like to acknowledge the College of Phar- macy and Nutrition, University of Saskatchewan for the...

Ngày tải lên: 25/10/2012, 11:15

... During the therapeutic process, real-time estimation of the therapeutic effect was carried out by the computer system through the graphic changes in the target field and the hyperecho of the ... and use of laboratory animals from the Ministry of Science and Technology of the People’s Republic of China. Instruments HIFU ablation was performed with a HIFU tu-...

Ngày tải lên: 25/10/2012, 11:18

... as the high- affinity peak of wild-type LPL. The LPL in the eluate was detected by Western blot with LPL polyclonal antibody 7640. The fraction number of the high-affinity peak was the same for the ... structure of monomeric LPL was duplicated and superimposed on the crystal structure of the horse PL dimer. After the energy minimization by setting at iteration of 3...

Ngày tải lên: 21/02/2014, 15:20

... used. RESULTS The enzymatic activity and molecular stability of the mutants The catalytic activity and the molecular stability of the mutants were assayed because a change in either of these parameters ... the cleavages of the sites were in the order: Tyr146/Asn147 ! Phe114 ! other sites. The weakness of bands of fragments I and IV and the appearance of fr...

Ngày tải lên: 22/02/2014, 07:20

... found. Several of these were identified in previous studies of EGF, and the backbone fold of the chimera is clearly similar to those of the EGF structures. The presence of a backbone hydrogen bond from the ... fits to the set of distances. The main advantages of the procedure are simple: the details of the calibration are precisely and unambiguously defined, i...

Ngày tải lên: 22/02/2014, 07:20

... perturbation of the axial symmetry of the tensor, whereas axial symmetry, i.e. g xx % g yy << g zz is typically found for type 2 sites. The grouping of the g-values and the line-shape of the spectrum ... Vlaardingen, the Netherlands; 2 University of Groningen, Laboratory of Biophysical Chemistry, Groningen, the Netherlands; 3 Department of Molecular Physic...

Ngày tải lên: 22/02/2014, 07:20

... is not hydrolysed by a-amylases therefore, the products of hydrolysis are always b-glycosides. Selection of these glycosides as substrates has been based on their size (DP 4–10) and good yields ... three different series of b-maltooligosaccharide glycosides and they confirmed the theory of five subsites [8]. However, the crystal structure of PPA isozyme II, in complex with the...

Ngày tải lên: 08/03/2014, 09:20

... the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction by the protein was affected by this modification. One of the consequences of ... version of CCR5 was present on the surface of transfected cells, and had the expected molecular mass. To test whether the presence of the myc-his tag at the C-ter...

Ngày tải lên: 08/03/2014, 09:20

... & Beyreuther, K. (1999) Mechanism of the cleavage specifi- city of Alzheimer’s disease gamma-secretase identified by phenyl- alanine-scanning mutagenesis of the transmembrane domain of the amyloid ... structures at the level of the Ile32–Gly33 connec- tion. Closer inspection of the kink region reveals the presence of a type I b-turn centred on residues 25–26, whi...

Ngày tải lên: 08/03/2014, 09:20