Báo cáo y học: " SNFing HIV transcription" pps
Báo cáo y học: " SNFing HIV transcription" pps
... acetylation by p300 [14-16]. Inter- estingly, Tat acetylation on lysines 50 and 51 was found necessary for interaction with BRG1 [11], a catalytic subu- nit of SWI/SNF complexes (unfortunately, ... unactivated HIV promoter, is occupied by nuc-1 [1]. Consistently, the nuc-1-binding site on HIV LTR became highly susceptible to digestion by restriction enzyme Afl II when BRG1 and acetylated...
Ngày tải lên: 13/08/2014, 09:20
Báo cáo y học: " Modulating HIV-1 replication by RNA interference directed against human transcription elongation factor SPT5" ppsx
... repli- cation, as shown previously (Figure 5; [47]). Double- Analysis of cell viability by counting trypan blue-stained cellsFigure 3 Analysis of cell viability by counting trypan blue-stained cells. ... 2001, 276:12951-12958. 26. Kim YK, Bourgeois CF, Isel C, Churcher MJ, Karn J: Phosphoryla- tion of the RNA polymerase II carboxyl-terminal domain by CDK9 is directly responsible for human imm...
Ngày tải lên: 13/08/2014, 13:20
Báo cáo y học: "Alkylating HIV-1 Nef - a potential way of HIV intervention" ppsx
... exactly equal to that of a one TPCK-alkylated Cys. Thus, the collective MS data proved that TPCK alkylates Cys55 and Cys206 but not Cys142 or any His residues. TPCK alkylation at Cys55 severely impaired ... (N-a-p-tosyl-L-phenylalanine chloromethyl ketone) and TLCK (N-a-p-tosyl-L-lysine chloromethyl ketone) are alkylation reagents that can chemically mod- ify side chains of specific His or Cy...
Ngày tải lên: 10/08/2014, 05:21
Báo cáo y học: "The HIV-1 Rev/RRE system is required for HIV-1 5’ UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particle" ppsx
... at the University of North Caro- lina Flow Cytometry Core Facility. Data was analyzed with Summit v4.3.01 software (Dako). Luciferase Assays Luciferase lysates were prepared by pelleting transfected 293T ... (Figure 3A), not in creases in cytoplasmic RNA (Figure 3B). Notably, however, the cytoplasmic levels of RNA may vary between experi- ments, which may be partially due to transfecti on var...
Ngày tải lên: 13/08/2014, 01:21
Báo cáo y học: " The HIV RNA setpoint theory revisited" pps
... Study: Changes in T and Non-T Lymphocyte Subsets Following Seroconver- sion to HIV- 1: Stable CD3 + and Declining CD3 - Populations Suggest Regulatory Responses Linked to Loss of CD4 Lym- phocytes. ... previ- ously used to investigate the causal pathways of the effects of age and three genetic cofactors on AIDS development [16]. Laboratory methods All CD4 lymphocyte counts were obtained pr...
Ngày tải lên: 13/08/2014, 05:22
Báo cáo y học: "Isolated HIV-1 core is active for reverse transcription" pptx
... Central yours — you keep the copyright Submit your manuscript here: http://www.biomedcentral.com/info/publishing_adv.asp BioMedcentral Retrovirology 2007, 4:77 http://www.retrovirology.com/content/4/1/77 Page ... mate- rial for analyses, a fresh equilibrium gradient scaled up approximately 20-fold was performed (Fig. 1C–F), and fractions were then analyzed by western analysis using purified...
Ngày tải lên: 13/08/2014, 05:22
Báo cáo y học: "Chipper: discovering transcription-factor targets from chromatin immunoprecipitation microarrays using variance stabilization" ppsx
... six (ICY1, HOR7, YPR127W, DPM1, POS5, and RSN1) also scored highly (above 2.0) without the tag, indicat- ing that they bind non-specifically. In fact, only POS5 scored in the top 100 by our method. ... high (y i >> α i ), yet transforms smoothly to the difference between channels when both are low. This allows direct comparison between any two datapoints, even when they belong to hl...
Ngày tải lên: 14/08/2014, 15:20
Tài liệu Báo cáo Y học: Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins doc
... previously [25]. 35 S-Labeled Sp1 and Sp3 were synthesized in a TNT Reticulocyte Lysate System (Promega Co.), according to the manufacturer’s protocol. Cell culture, transfections and assays of ... transfection. Promoter activity was determined with a Luciferase Assay System (Promega Co.) as described by the manufacturer. Electrophoretic mobility shift assays (EMSAs) Nuclear extracts were ......
Ngày tải lên: 22/02/2014, 07:20
Báo cáo Y học: Activation of transcription of the human presenilin 1 gene by 12-O-tetradecanoylphorbol 13-acetate pdf
... gene by 12- O -tetradecanoylphorbol 13-acetate Martine Pastorcic 1 and Hriday K. Das 1,2 1 Department of Pharmacology & Neuroscience and 2 Department of Molecular Biology & Immunology, and ... hdas@hsc.unt.edu Abbreviations: EMSA, Electrophoretic mobility shift assays; FAD, familial early onset Alzheimer’s disease; GAPDH, glyceraldehyde-3 phosphate dehydrogenase; JNK, c-Jun N-terminal...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo Y học: Activation of transcription through the ligand-binding pocket of the orphan nuclear receptor ultraspiracle docx
... fluorescence binding assay and a transfection transcriptional assay that is activated by treatment with methyl epoxyfarnesoate will be very useful in identifying new, higher-affinity ligands for USP. ... terminal carboxylate oxygens in dark blue, adapted from Egea et al. [52]). (C) This shows methyl epoxyfarnesoic acid (yellow carbon backbone and blue terminal carboxylate oxygens) lain manually...
Ngày tải lên: 23/03/2014, 21:20