Báo cáo y học: " Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120" docx

... purposes) Retrovirology Open Access Research Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120 James M Binley 1 , Stacie Ngo-Abdalla 2 , Penny Moore 3 , ... effectively, if imper- fectly, by measuring Fc-gp120-DC-SIGN binding. Binding of Fc-gp120 chimeras to cellular CCR5Figure 3 Binding o...

Ngày tải lên: 13/08/2014, 09:20

... purposes) Retrovirology Open Access Correction Correction: Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120 James M Binley 1 , Stacie Ngo-Abdalla 2 , ... 1 Figure 4. 2G12 neutralizes HIV- 1 JR-CSF effectively in a post- CD4 assay format. The neutralization activity of mAbs A) b12, B) X5, and C)...

Ngày tải lên: 13/08/2014, 09:20

... residue catalyzed by deoxyhypusine synthase and deoxyhypusine hydroxylase (DOHH). DOHH activity is inhibited by two clinically used drugs, the topical fungicide ciclopirox and the systemic medicinal ... serum transferrins by 1, 2-dimethyl-3-hydroxypyridin-4-one, 1- hydroxypyridin-2-one and acetohydroxamic acid. Biochim Bio- phys Acta 1998, 1387:89-102. 85. Barradas MA, Jeremy JY, Kontog...

Ngày tải lên: 12/08/2014, 23:22

... vitro systems [23]-we focused on the nuclear import of IN protein using yeast and mammalian Binding of IN to importin α as estimated by an ELISA-based systemFigure 6 Binding of IN to importin α as ... interaction with the impor- tin α receptor. IN interaction as observed by the BiFC assay systemFigure 5 IN interaction as observed by the BiFC assay system. EGY48 yea...

Ngày tải lên: 12/08/2014, 23:22

... microscopically. b-galactosidase enzyme assay Cell lysates were prepared b y using Reporter Lysis Buf- fer (Promega, Mannheim, Germany). To perform 96- well plate b-galactosidase assays 50 μl of cell lysates ... up-regulation of GliPR by HIV- 1 and the early significant inhibition of HIV- 1 replication mediated by knockdown of GliPR reveal GliPR as an important HIV- 1 d...

Ngày tải lên: 12/08/2014, 23:23

... that of PBLs, limiting the number of independent replicas per experiment that could be per- formed. To obtain an accurate IC 50 for inhibition of HIV Inhibition of HIV- 1 infectivity by non-cytotoxic ... regulatory subu- nits, cyclin T1, cyclin T2a, cyclin T2b or cyclin K, with cyc- lin T1 being the predominantly associated cyclin in many cell types [11,12]. The kinase ina...

Ngày tải lên: 13/08/2014, 05:22

... and protease enzymes by using nucl- eoside analogues as enzymes inhibitors, and their combi- nation, known as highly active antiretroviral therapy (HAART), has markedly decreased mortality and morbid- ity ... activated LTR-Luciferase activity and specific TAR RNA binding by Tat-PFigure 6 Inhibitory effect of Tat-P on TAT activated LTR-Luciferase activity and specific TAR RNA bind...

Ngày tải lên: 13/08/2014, 09:21

... activity of NF-κB-independent genes by interacting with nuclear co-repressors, histone acetyltrans- ferases and deacetylases [23,24]. Further studies are required to clarify novel activities of IκB-α ... transfected into 293T cells to analyse for the expres- sion of HIV- 1 proteins and IκB-αS32/36A polypeptide by immunoblotting (Fig. 1 B, C). As expected the IκB-αS32/ 36A-FLA...

Ngày tải lên: 13/08/2014, 13:20

... prior to analy- sis of DNA content by flow cytometry as described [62]. Three days after transfection, the remaining HPB-ALL cells were rinsed in PBS and analyzed by flow cytometry for exposure of ... tryptophan, leucine, and histidine for auxotrophy analysis, and on Whatman 40 filters for β-galactosidase (β-gal) activity assay [12]. This latter assay was monitored by incubation...

Ngày tải lên: 13/08/2014, 05:22

... possibility of post entry inhibition. We tested for inhibition of infection with VSV- G pseudotyped HIV- 1, which has been used to bypasses any entry restrictions [31,35]. Treatment with increasing doses ... h at a ratio of 1:1, and examined for cell -to- cell fusion and syncytia formation by phase contrast microcopy (A-F) or by luciferase expression (H). As expected, many l...

Ngày tải lên: 10/08/2014, 05:20