Báo cáo y học: " Wild-type and central DNA flap defective HIV-1 lentiviral vector genomes: intracellular visualization at ultrastructural resolution levels" doc

... labeled vector DNA. BioMed Central Page 1 of 7 (page number not for citation purposes) Retrovirology Open Access Short report Wild-type and central DNA flap defective HIV-1 lentiviral vector genomes: ... intracellu- lar visualization of HIV-1 DNA genomes by in situ DNA hybridization with electron microscopy. The data we obtained reveal that lack of the centr...

Ngày tải lên: 13/08/2014, 09:20

... scheme means that two methyl groups are always introduced, one slowly by Dnmt3a or by de novo methylation catalyzed by Dnmt1 and the second one very fast. Therefore, this effect can only double the rate ... experimentally investigate a model for de novo methylation of DNA that postulates functional cooperation of Dnmt1 and Dnmt3a. Purified Dnmt1 and Dnmt3a enzymes were employed to meth...

Ngày tải lên: 21/02/2014, 01:21

... that binds to DNA and DNA L the complex between DNA and L. The intrinsic binding constant, K B , is defined by the equation [24,28,29]: K B ¼ y ð1 À y ð DNA 0 À ny½L 0 Þ ð14Þ Here, [DNA] 0 and ... DNA. MATERIALS AND METHODS Materials Calf thymus DNA (Sigma Chemical Co., MI, USA) was purified by ethanol precipitation and centrifugal dialysis and sheared by sonication...

Ngày tải lên: 24/03/2014, 04:21

... cytokines. Y Katsumata and SH carried out fluo- rescence-activated cell sorting analysis. CF and HI carried out proliferation assays. NK made contributions to the design and coordination of the study. ... Antigen-specific regulatory T cells develop via the ICOS-ICOS-ligand pathway and inhibit allergen-induced airway hyperreactivity. Nat Med 2002, 8:1024-1032. 28. Wiley RE, Goncharov...

Ngày tải lên: 09/08/2014, 07:20

... clinical and rheumatological data analyses. AZ, CB and EC conducted assessment of Chlamydia trachomatis serology and DNA extraction. BJ and JS participated in the design and coordination of the study, ... denaturation at 94°C for 5 min- utes, followed by 30 cycles of denaturation at 94°C for 1 minute, primer annealing at 59°C for 1 minute and extension at 72°C for 1.5 mi...

Ngày tải lên: 09/08/2014, 10:23

... mildly icteric and urinalysis revealed 1+ of bilirubin. Blood pressure was 90/45 mmHg and pulse rate was 92 beats per minute. Examination of the chest and abdomen was unremarkable. Fundal examination ... case describes a young woman with severe anaemia caused by very poor diet. Her visual acuity gradually dete- riorated over several weeks and it turned out she had a bilateral central...

Ngày tải lên: 11/08/2014, 23:21

... differen- tially expressed genes exhaustively analyzed by DNA microarray together with bioinformatics analysis. Materials and methods Patients and healthy individuals Twenty-one patients (all women, ... Several ATPases and ATP synthases such as ATP2B1, ATP2B2, ATP5D, ATP5S, ATP6V1H, ATP8A2, and ATP10B were also significantly downregu- lated (unpaired Mann-Whitney test, P < 0.01)...

Ngày tải lên: 12/08/2014, 15:23

... complementation group (FANCA, D2, E and G). FANC genes mediate a stress related signal- ing pathway that allows a normal cell to surmount certain types of damage induced in DNA, principally interstrand crosslinks ... then isolated by affinity purifi- cation of the S peptide and the complexes separated on SDS-PAGE and subjected to immunoblotting with anti- DNA- PKcs. Endogenous DNA- P...

Ngày tải lên: 13/08/2014, 05:21

... the DNA and RNA polymerases, after refinement by optimization of geometry and MD simulations, had their structures validated by PROCHECK and ANOLEA (Figure 5). The Ramachandran plot showed that ... acids Asp537 and Asp812 (Palm), and Tyr639 and Lys631 (Fin- gers) of the template. The presence of these residues (Asp, Tyr, and Lys) in this region is a sign in this group of polym...

Ngày tải lên: 13/08/2014, 16:20

... metagenomic and diversity investigations is to identify and remove pyrosequencing reads that are likely to be incorrect. For example, Gilbert et al. [9], in a study of ancient woolly mammoth mitochondrial ... between pyrosequences and their cognate refer- ence sequences identified signatures of low quality data. Results Read accuracy We obtained 340,150 reads that passed the GS20 qualit...

Ngày tải lên: 14/08/2014, 07:22