Báo cáo y học: " Tumour necrosis factor-α stimulates HIV-1 replication in single-cycle infection of human term placental villi " docx

... the role played by TNF receptor II in regulation of Cyp7b activity. Thus, in order to study which signaling path- ways are involved in TNF-α-induced Cyp7b activity, we used different inhibitors ... not result in convincing changes in TNF-α-induced Cyp7b mRNA expression. We then determined Cyp7b enzymatic activity in FLS through the detection of 7α-OH-DHEA. Presence of TNF-α...

Ngày tải lên: 09/08/2014, 07:20

... Central Page 1 of 9 (page number not for citation purposes) Retrovirology Open Access Research Tumour necrosis factor-α stimulates HIV-1 replication in single-cycle infection of human term placental villi ... MTCT. Indeed, HIV-1 MTCT occurs mainly with R5 viruses. Addi- Infection with VSV-G or delta-Env pseudotyped HIV-1 of placental chorionic villi...

Ngày tải lên: 13/08/2014, 09:20

... signal-regulated kinase (ERK), and (c,f,i) c-Jun amino-terminal kinase (JNK) were stained in both the synovial pannus (panels a–c) and the synovial lining layer (panels d–f) of human tumour necrosis ... activated in 24 ± 4% of synovial cells of hTNFtg mice whereas it was only activated in 5 ± 1% in the synovium of wild-type mice (Fig. 2j). Similarly, activation of ERK...

Ngày tải lên: 09/08/2014, 07:20

... automated radiogrammetry. Moreoever, automated radiogrammetry using hand X-rays shows a strong correlation with BMD assessed by dual energy X-ray absorptiometry [13]. In summary, studies like that by Marotte ... sulphasalazine, antimalarials and injectable gold. Of interest, the manual technique of metacarpal morphometry employed in these three old studies is identical with that measu...

Ngày tải lên: 09/08/2014, 10:20

... antibody at 4°C overnight with 1 µg/ml recombinant TNF-α. Endo- toxin contamination was excluded by assaying secretion in the presence of 5 µg/ml endotoxin inhibitor polymyxin B sulphate. Preliminary ... Previous studies of TNF-α in the context of SSc have largely focused on measurements of cytokines in serum [14]. One previous publication has explored a variety of cytokines,...

Ngày tải lên: 12/08/2014, 18:20

... van den: Role of interleukin-1, tumor necrosis factor alpha, and interleukin-6 in cartilage proteoglycan metabolism and destruction. Effect of in situ blocking in murine antigen- and zymosan-induced ... that cytokines can be involved in cartilage tissue or proteoglycan degradation [6]. It has recently been shown in a serum-containing porcine in vitro model that these cytokin...

Ngày tải lên: 09/08/2014, 14:22

... therefore, investigated this proposal and the macrophage signaling pa thways underlying it. Methods: Caspase-3 activity, annexin-V binding/7-aminoactinomycin D (7-AAD) exclusion and cell-cycle analysis were ... (M-CSF) was obtained from R&D Systems (Sydney, NSW, Australia). Annexin V conjugated to phycoery- thrin (Annexin V-PE) and 7-amino-actinomycin (7-AAD) were from BD Biosciences (Syd...

Ngày tải lên: 12/08/2014, 15:22

... to B lymphocytes, FDCs, or the combination of B lymphocytes and FDCs at the initiation of cul- ture, and supernatants were harvested after 7 days of incubation. In keeping with the purity of our FDCs, ... predominant action of WEB 2170 is on B-lymphocyte PAFR. Discussion The interaction between FDCs and B lympho- cytes is a crucial one for the generation of high- affinity antibo...

Ngày tải lên: 08/08/2014, 20:23

... onset of hepatic insulin resis- tance typically precedes peripheral insulin resistance in humans [39]. The stimulation of gluconeogenesis occurs invariably as a result of increased activity of PEPCK, ... 1A,B). The inhibition of insulin signalling in the liver is pri- marily reflected by the attenuation of the insulin-medi- ated inhibition of gluconeogenic gene expression. As...

Ngày tải lên: 16/03/2014, 02:20

... further expression of cytokines and chemokines by synovial cells and chondro- cytes, thereby sustaining a renewal of local inflammatory mediators (reviewed in [9,10]). The presence of TNF-α cor- relates ... were independent of protein kinase C signaling, but were dependent on MEK1/2 activity. Finally, we analyzed the involvement of Sox- 9 using a reporter construct of the 48 bas...

Ngày tải lên: 09/08/2014, 06:22