ENZYME KINETICS A MODERN APPROACH – PART 9 docx
ENZYME KINETICS A MODERN APPROACH – PART 9 docx
... less than either valine and serine mutants, which had comparable catalytic constants. Compared to valine and serine, alanine has the smallest van der Waals volume and accessible surface area. Therefore, ... amino acids differ in their van der Waals volumes, accessible surface areas, polarities, and allowable energy levels on Ramachandran plots for individual amino acids. Rate constants for th...
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ENZYME KINETICS A MODERN APPROACH – PART 1 pps
... call 1-800-CALL-WILEY. Library of Congress Cataloging-in-Publication Data: Marangoni, Alejandro G., 196 5- Enzyme kinetics : a modern approach / Alejandro G. Marangoni. p. ; cm. ISBN 0-471-1 598 5 -9 ... 28 1.6.1.2 Least-Squares Minimization (Regression Analysis) / 29 1.6.2 Exact Analytical Solution (Non-Steady-State Approximation) / 39 1.6.3 Exact Analytical Solution (Steady-Sta...
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ENZYME KINETICS A MODERN APPROACH – PART 2 pptx
... Changes in the reaction rate constant for an acid/base-catalyzed reaction as a function of pH. A negative sloping line (slope =−1) as a function of increasing pH is indicative of an acid-catalyzed ... for example, the reaction A → B → C. Usually, equations in differential or algebraic form are fitted to indi- vidual data sets, A, B, and C and a set of parameter estimates obtained. 2...
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ENZYME KINETICS A MODERN APPROACH – PART 3 pps
... statistic: χ 2 = n i=1 (y i −ˆy i ) 2 s 2 i = n i=1 w i (y i −ˆy i ) 2 (1.125) Consider a typical experiment where the value of a dependent variable is measured several times at a particular value of the independent variable. From these repeated determinations, a mean and variance ... Exact Analytical Solution (Non-Steady-State Approximation) Exact analytical solutions for the rea...
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ENZYME KINETICS A MODERN APPROACH – PART 4 pptx
... is appropriate. When more than two values are compared, a one-way analysis of variance (ANOVA), TABLE 4.2 Rate of Hydration of Fumarate to Malate by Fumarase at various Substrate Concentrations a Velocity ... V ∗ max and K ∗ s correspond, respectively, to apparent enzyme maxi- mum velocity and apparent enzyme substrate dissociation constant at a particular pH. For the model above, V...
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ENZYME KINETICS A MODERN APPROACH – PART 5 pptx
... cooperativity, which can have noninteger values. Estimates of the parameters k and n are obtained using standard non- linear regression procedures available in most modern graphical software packages. ... as v V max = [B] K + [B] (7.12) where V max = V max [A] K BA + [A] (7.13) and K = K A s K AB + K AB [A] K BA + [A] = K AB (K A s + [A] ) K BA + [A] (7.14) From determi...
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ENZYME KINETICS A MODERN APPROACH – PART 6 pptx
... exponential term, and the shape of the curve approaches that of a straight line. Valuable information can be gained from analysis of the early and late stages of this reaction. 11.2.1 Early Stages ... plot for an interfa- cial enzyme. various parameters in Eq. (10 .9) on the velocity of a reaction catalyzed by an interfacial enzyme. As the enzyme interface dissociation constant increa...
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ENZYME KINETICS A MODERN APPROACH – PART 7 ppsx
... treatment above assumes that there are no differences in heat capac- ity between native and denatured states of an enzyme and that the heat capacity remains constant throughout the temperature ... stability, enzyme solutions are incubated at a particular temperature and aliquots removed at the appro- priate times. Enzyme activity in these samples is then measured at the enzyme s temp...
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ENZYME KINETICS A MODERN APPROACH – PART 8 ppt
... Finer-Moore, Y. Wataya, and D. V. Santi, Biochemistry 33, 1508 6–1 5 094 ( 199 4). Groutas, W. C., R. Kuang, R. Venkataraman, J. B. Epp, S. Ruan, and O. Prakash, Biochemistry 36, 473 9 4 750 ( 199 7). Groutas, W. ... Loughran, and M. D. Walkinshaw, J. Biol. Chem. 274, 2 490 1–2 490 5 ( 199 9). Vilain, A C., V. Okochi, I. Vergely, M. Reboud-Raveax, J P. Mazalelyrat, and M. Wakselman, Bio...
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ENZYME KINETICS A MODERN APPROACH – PART 10 pps
... enzyme- catalyzed reactions, 7 9 8 2 transient phases and, 13 2–1 35 Maximum reaction velocity (V max ), 49, 8 0–8 2, 92 , 93 9 5, 102 apparent, 91 , 94 , 95 , 9 6 9 7, 9 9 1 00, 10 0–1 01 appropriate [S] ranges ... Teo, K. M. Lamb, L. J. Harris, and R. Y. Yada ( 199 8). Protein Peptide Lett. 5, 1 9 2 6. Tanaka, T., and R. Y. Yada ( 199 6). Biochem. J. 315, 44 3–4 46. Tanak...
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