ENZYME KINETICS A MODERN APPROACH – PART 6 pptx
ENZYME KINETICS A MODERN APPROACH – PART 6 pptx
... exponential term, and the shape of the curve approaches that of a straight line. Valuable information can be gained from analysis of the early and late stages of this reaction. 11.2.1 Early Stages ... plot for an interfa- cial enzyme. various parameters in Eq. (10.9) on the velocity of a reaction catalyzed by an interfacial enzyme. As the enzyme interface dissociation constant increas...
Ngày tải lên: 13/08/2014, 07:20
ENZYME KINETICS A MODERN APPROACH – PART 2 pptx
... hydroxyl ion–catalyzed reaction, k HA is the rate constant for the undissociated acid-catalyzed reaction, and k A − is the rate constant for the conjugate base–catalyzed reaction. 32 TOOLS AND TECHNIQUES ... for example, the reaction A → B → C. Usually, equations in differential or algebraic form are fitted to indi- vidual data sets, A, B, and C and a set of parameter estimates obtained...
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ENZYME KINETICS A MODERN APPROACH – PART 4 pptx
... is appropriate. When more than two values are compared, a one-way analysis of variance (ANOVA), TABLE 4.2 Rate of Hydration of Fumarate to Malate by Fumarase at various Substrate Concentrations a Velocity ... results in an apparent decrease in V max and an apparent increase in K s . The rate equation for the formation of product, the dissociation constants for enzyme substrate (ES and ES...
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ENZYME KINETICS A MODERN APPROACH – PART 5 pptx
... cooperativity, which can have noninteger values. Estimates of the parameters k and n are obtained using standard non- linear regression procedures available in most modern graphical software packages. ... as v V max = [B] K + [B] (7.12) where V max = V max [A] K BA + [A] (7.13) and K = K A s K AB + K AB [A] K BA + [A] = K AB (K A s + [A] ) K BA + [A] (7.14) From determi...
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ENZYME KINETICS A MODERN APPROACH – PART 1 pps
... customer service, call 1-800-CALL-WILEY. Library of Congress Cataloging-in-Publication Data: Marangoni, Alejandro G., 1 965 - Enzyme kinetics : a modern approach / Alejandro G. Marangoni. p. ; cm. ISBN ... 28 1 .6. 1.2 Least-Squares Minimization (Regression Analysis) / 29 1 .6. 2 Exact Analytical Solution (Non-Steady-State Approximation) / 39 1 .6. 3 Exact Analytical Solution (Stead...
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ENZYME KINETICS A MODERN APPROACH – PART 3 pps
... statistic: χ 2 = n i=1 (y i −ˆy i ) 2 s 2 i = n i=1 w i (y i −ˆy i ) 2 (1.125) Consider a typical experiment where the value of a dependent variable is measured several times at a particular value of the independent variable. From these repeated determinations, a mean and variance ... Exact Analytical Solution (Non-Steady-State Approximation) Exact analytical solutions for the rea...
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ENZYME KINETICS A MODERN APPROACH – PART 7 ppsx
... treatment above assumes that there are no differences in heat capac- ity between native and denatured states of an enzyme and that the heat capacity remains constant throughout the temperature ... stability, enzyme solutions are incubated at a particular temperature and aliquots removed at the appro- priate times. Enzyme activity in these samples is then measured at the enzyme s temp...
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ENZYME KINETICS A MODERN APPROACH – PART 8 ppt
... K i of an alter- nate substrate. Addition of an alternate substrate inhibitor to an enzyme assay results in an exponential decrease in rate to some final steady- state turnover of substrate (Fig. ... H. Abu-Soud, W. A. Francisco, and F. M. Raushel, J. Am. Chem. Soc. 113, 8 56 0–8 561 (1991). Clemente, A. , A. Domingos, A. P. Grancho, J. Iley, R. Moreira, J. Neres, N. Palma, A. B. San...
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ENZYME KINETICS A MODERN APPROACH – PART 9 docx
... than either valine and serine mutants, which had comparable catalytic constants. Compared to valine and serine, alanine has the smallest van der Waals volume and accessible surface area. Therefore, ... determinations ± stan- dard deviation. b ss1, substrate consisting of the peptide lysine–proline–alanine–glutamic acid–phenylalanine– phenylalanine (NO 2 )–alanine–leucine. c ss2, substrate con...
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ENZYME KINETICS A MODERN APPROACH – PART 10 pps
... INDEX Abbreviations, table for mutation research, 21 4–2 16 Absolute reaction rate theory, 2 3–2 6 Acid–base catalysis, 2 0–2 3 Acidity constant (K a ), 2 0–2 1 Activation, 103 Activation curves, 20 0–2 02. ... 17 7–1 79 appropriate [S] ranges for, 18 1–1 84 consistency within, 18 4–1 91 enzyme catalytic parameters in, 5 8 6 0 in enzyme characterization, 17 4–1 75 f...
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