Báo cáo y học: " The histone chaperone protein Nucleosome Assembly Protein-1 (hNAP-1) binds HIV-1 Tat and promotes viral transcription" potx

... also known as Tat- associated protein (TAP) [53,54]; ribosomal protein S4 and the histone chaperone NAP-1 (Nucleosome Assembly Protein- 1). The proteomic analy- sis was repeated and the results were ... transactivator that binds an RNA element (TAR) positioned at the 5' end of the primary proviral transcript [40]. Tat activates HIV-1 transcription by pro...

Ngày tải lên: 13/08/2014, 06:20

... differences in the activity of these two proteins have been reported. In this study, we used murine CtBP2 [25], but it is likely that the results will also apply to CtBP1, and therefore, in some ... early stages of differentiation and to the lymphoid compartment in the adult [2,3]. The Ikaros gene codes for a protein with six zinc fingers that comply with the Kru ¨ ppel C2-H2...

Ngày tải lên: 21/02/2014, 01:21

... significantly different from the theoritical pI values calculated for the isolated protein, namely 5.9 for p67phox and 9.1 for p47phox. This is readily explained by the association of these proteins ... be further processed Fig. 1. Coimmunoprecipitation of the cytosolic phox proteins p67phox, p47phox and Rac, and the S100A8/A9 protein. The proteins from nonactivated bovine...

Ngày tải lên: 18/03/2014, 01:20

... Therefore, alteration of the charge of the protein could be induced at the vicinity of the monolayer. A difference in the orientation of the protein moiety during the insertion process into the ... interactions with the lipid layers. Our results indicate that the penetration of the protein into the lipid monolayer is also influenced by the hydrophobic interactio...

Ngày tải lên: 31/03/2014, 21:21

... Although the viral proteins nucle- ocapsid, matrix, integrase, tat, nef and vif may participate in the regulation and/ or efficiency of reverse transcrip- tion [1-6], synthesis of the nascent HIV-1 ... internal RRMs, the internal and C-terminal RRMs, or the C-terminal RRM only. Results: All four HuR proteins were purified and characterized by biophysical methods. They ar...

Ngày tải lên: 12/08/2014, 23:23

... group of the invariant tyrosine from the carboxyl terminus of the SET domain (Figure 1). These carbonyl and hydroxyl oxygens have been proposed to facilitate the transfer of the methyl group ... L I LI ASRDI AAGEEL LY DYGDRS AdoMet AdoMet and target Lys Catalytic site F /Y switch F /Y switch Pseudoknot Salt bridge AdoMet with several structurally conserved carbonyl oxygens as...

Ngày tải lên: 14/08/2014, 14:21

... recently showed that Msn2/4p activity is inhibited by NuA4-dependent histone acetylation. This raises the possibil- ity that histone deacetylation by Rpd3p counteracts the inhib- itory effects ... CA, USA) and a Guava flow cytometer. The plot shows the growth score (the sum of all viability scores normalized to the starting viability score in untreated cells) for wild-type a...

Ngày tải lên: 14/08/2014, 21:20

... committees many years before the initiation of the NARP. Even in these selected centres already apply- ing local antibiotic restriction policies the utilisation of many of the restricted antibiotics ... study performed in four year period, which included two years before and after of the initiation of NARP in 2003. The study in- cluded the data obtained from all of the...

Ngày tải lên: 25/10/2012, 11:00

... (k cat /K m )forp-hydroxyphenylgly- cine methyl ester and phenylglycine methyl ester that were similar to the wild-type values, whereas the specificity con- stants for p-hydroxyphenylglycine amide and phenylglycine amide ... the methyl ester or the amide as acyl donor. It appeared that 6-APA and 7-ADCA were able to efficiently deacylate the phenylglycyl- and p-hydroxyphe- nylg...

Ngày tải lên: 17/03/2014, 23:20

... peptide-independent pathway and calls for further investigation on this subject. Enzyme activity and specificity In the standard tricaprylin hydrolysis assay, rPFL displayed highest activity at 29 °C and pH 8.0, ... with the solvent [3]. Both factors are of importance for cold activity. On the other hand, the presence of disulphide bridges may increase the rigidity of the pr...

Ngày tải lên: 24/03/2014, 04:21