Báo cáo y học: "Activation of HIV-1 expression and replication by cGMP dependent protein kinase type 1-β (PKG1β)" docx

... purposes) Retrovirology Open Access Short report Activation of HIV-1 expression and replication by cGMP dependent protein kinase type 1-β (PKG1β) Jia Hai Lee, Venkat RK Yedavalli and Kuan-Teh Jeang* Address: ... (PKG1-β) and cGMP analogues on transcriptional activity and replication of human immunodeficiency virus type 1 (HIV-1) was investigated. Transfec...

Ngày tải lên: 13/08/2014, 05:22

... logarithmic mode and the cytofluorimetric data were analyzed by the FLOWIN software (written by M. Emri & L. Balkay, University of Debrecen, Positron Emission Tomography Center, Hungary). RESULTS As ... of cardiovascular drugs on ATPase activity of P-glycoprotein in plasma membranes and in purified reconstituted form. Biochim. Biophys. Acta 1369, 85–93. Ó FEBS 2002 Catalytic c...

Ngày tải lên: 08/03/2014, 23:20

... perinuclear/centrosomal site; analysis by confocal microscopy and FRET. Traffic 2005. in press 15. Kaye JF, Lever AML: Nonreciprocal packaging of human immu- nodeficiency virus type 1 and type 2 RNA: a possible ... to Transduction of Rat primary mixed glial cultures with Lentiviral vectors based on HIV-1 packaged by HIV-1 Gag-pol(A), HIV-1 +cPPT vector packaged by HIV...

Ngày tải lên: 13/08/2014, 09:21

... regulated by the myo- genic differentiation factors and serum response factor (SRF). The myogenic transcription factors myogenin and myogenic differentiation 1 (MyoD) bind to regions upstream of the ... Transcriptional and post-transcriptional impact of toxic RNA in myotonic dystrophy. Hum Mol Genet 2009, 18(8):1471-81. 9. Chen K, Rajewsky N: The evolution of gene regulation b...

Ngày tải lên: 18/06/2014, 16:20

... down-regulation of protein kinase C with long exposure to TPA observed in many cell types [29]. In order to analyze further the mechanism of activation we have tested the effect of TPA on the activity of ... fractionated by electrophoresis on 12% (w/v) polyacrylamide gels and analyzed by Western Fig. 1. TPA increases the level of PS1 mRNA in SK-N-SH cells. (A) SK-N-SH cell...

Ngày tải lên: 08/03/2014, 09:20

... lg of platelet protein and 0.5 lg of GST and GST±Rab p roteins; (B) an [a- 32 P]GTP overlay [17] of a nitrocellulose blot of a gel containing 40 lg of platelet protein, 0.5 lgofGSTand0.5lgofGST±Rab proteins, ... GST±Rab31 and Fig. 7. Puri®cation and properties o f wild -type and mutant Rab31 and Rab32 expressed a s GST±fusion proteins. Samples of platelet particu-...

Ngày tải lên: 08/03/2014, 16:20

... homodimer (upper band) and in part as a monomer (lower band) to a 4DR12 motif probe (identification of monomer and homodimer bands was made by comparative analysis of binding by other dimer-enriched ... similar results. Modelling of hRXRa and D. melanogaster USP Tertiary conformation of human RXRa and D. melano- gaster USP was analyzed by RASMOL software, using the coordi...

Ngày tải lên: 23/03/2014, 21:20

... whereby it i s translocated back to the cytoplasm [19,39]. While NFAT dephosphorylation is mediated by calcineurin, rephosphorylation is catalyzed by a variety of serine kinases, such as glycogen ... glycogen synthase kinase- 3, ERK, p38, casein kinase- 2, and c-Jun N-terminal kinase [40–43]. These enzymatic activities may b e targeted by RA in order t o block NFAT dephosphoryl...

Ngày tải lên: 24/03/2014, 03:21

... dimerization thereby indirectly influencing binding of the catalytic calcium for wild -type OMPLA. Subsequently, it was investigated whether the type of detergent in the kinetic assays had any effect on ... activity of the covalent OMPLA dimer was independent of type and concentration of detergent in micellar systems. In such systems, the cova- lent OMPLA dimer invariantly dis...

Ngày tải lên: 24/03/2014, 03:21

... sequence-specific, and concentration- dependent inhibition of fusion protein expression. At 0.5 l M , AS-6 and AS-8 inhibited EGFP–GSTM1 expression by 95 ± 4% and 81 ± 6%, respectively. AS-5 and AS-10 ... accurate determination of the effects of AS-ODNs by flow cytometry. C-terminal fusion constructs of GSTM1 and GSTM2 with EGFP (named pEGFP-M1 and pEGFP-M2, respe...

Ngày tải lên: 31/03/2014, 15:20