... this article as: Zhao et al.: Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR. Virology Journal 20 10 7:374.Submit your next manuscript to BioMed Centraland take ... 7:374http://www.virologyj.com/content/7/1/374Page 5 of 5 SHOR T REPOR T Open Access Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCRKai Zhao1,3, Fangting Han4, Yong Zou 2, 3, Lianlong Zhu1,3, ... dTTP, dCTP and dGTP, 1 .25 U DNA polymerase,2mMMgCl 2 (TaKaRa, Dalian, China), 20 0 nM of eachprimer, and different quantities of the plasmid DNAtemplates. Amplifications were programmed as follows:onestepof94°Cfor5min,30cyclesof94°Cfor30s,60°C...