Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf

... genome of mutant cells. Clones 30-2 and 42-7 are resistant to MLV and HIV-1 infection We then quantified the resistance to infection of clones 30-2 and 42-7 by fluorescence cytometry relative to ... vector at an moi of 0.01, 0.1, 1 and 10 (the moi were Growth rates of parental and mutant cells and extent of HIV integrationFigure 2 Growth rates of parental...

Ngày tải lên: 13/08/2014, 05:22

... puri®ed to homogeneity by HPLC and digested with t rypsin. The tryptic peptides were separated by HPLC and some of them were totally or partially analyzed by Edman sequencing and/ or by MALDI-TOF mass ... online phenylthiohydantoin amino-acid analyser. F or MALDI-TOF analysis, 1 lL of tryptic digest or HPLC-puri®ed peptides was mixed with 1 lLof saturated solution of a-cyano-4-hy...

Ngày tải lên: 08/03/2014, 16:20

... preferentially positioned in prox- imity to the glycosylation site and especially in the )1 and/ or +3 positions [27]. According to the NetOGlyc server, predicting mucin-type O-glycosylations using ... the algorithm of Nielsen et al. [28], the extracellular region of bovine and human MUC15 offer 22 and 14 O-glycosylation sites, respectively. The majority of these potential...

Ngày tải lên: 24/03/2014, 04:21

... enzyme digestion, level of DNA methyla tion, production of hyaluronan, and phylogeny of the DNA polymerase gene. Taken together, all of these observa- tions indicate that CvV-BW1 is likely to ... intensity of band Z increased; with heat treatment at 100°C, only band Z was observed. The sizes of proteins in bands X, Y, and Z were estimated to be 370 kDa, 105 kDa, and 50...

Ngày tải lên: 12/08/2014, 01:21

... Reynolds SD, Xiao YQ, Riches DW, Plumb J, Vachon E, et al.: Dysfunctional cystic fibrosis transmembrane conductance regulator inhibits phagocytosis of apoptotic cells with proinflammatory consequences. ... analysis of inflammatory cells infiltrating the cystic fibrosis airway mucosa. Clin Exp Immunol 2001, 124(1):69-76. 35. Livraghi A, Randell SH: Cystic fibrosis and other respi...

Ngày tải lên: 12/08/2014, 11:22

... activity. P I and P II promoted hem- agglutination of human erythrocytes at 22.5 and 27.5 ngÆmL )1 , respectively. Although P I showed only activity towards human erythrocytes, P II was able to agglutinate ... III and P IV) were isolated by affinity, ion exchange and chromatofocusing chromatographies, and investigated with respect to toxicity and sugar binding specificity....

Ngày tải lên: 18/02/2014, 16:20

... C1 atom. Tyr183 is believed to assist Asp125 in this function by hydrogen bonding to the carbonyl oxygen of the N-acetyl group. In this specific orien tation the N- acetyl carbonyl oxygen atom ... tructural and k inetic data sh ow that Asp125 and Tyr183 contribute to catalysis by positioning the carbonyl oxygen of the N-acetyl group near to the C1 atom. This allows the stabiliz...

Ngày tải lên: 22/02/2014, 04:20

... d-cysteine of 0.25 mm. Only d-cysteine but not l-cysteine was converted by d-CDes to pyruvate, H 2 S, and NH 3 . The activity was inhibited by aminooxy acetic acid and hydroxylamine, inhibitors ... such as N-glycosylation, tyrosine sulfation, phosphorylation, myristylation, and amidation sites, all of them are characterized by a high probability of occurrence. Enzyme activity o...

Ngày tải lên: 16/03/2014, 18:20

... order to more systematically test this hypothesis, we elected to eliminate the possibility of disulphide bond formation genetically by replacement of residues Cys22 and Cys82 with alanine and valine, ... action of cytosolic chaperones for their delivery to the mitochondrial surface [20,21]. The human homologue of Tom70 has only recently been identified [22], and attempts...

Ngày tải lên: 17/03/2014, 10:20

... proPT18, residues )18 to )1 of proprothrombin; proPT28, residues )18 to +10 of proprothrombin; proFIX18, resi- dues )18 to )1 of proFactor IX; proFIX28, residues )18 to +10 of proFactor IX; pro-e-TxIX/12, ... proprothrombin), proFIX18 (residues )18 to )1 of proFactor IX), proFIX28 (residues )18 to +10 of proFactor IX), pro-e-TxIX/12 (residues )12 to )1ofe-TxIX precur...

Ngày tải lên: 17/03/2014, 10:20