Báo cáo y học: "Contribution of the C-terminal region within the catalytic core domain of HIV-1 integrase to yeast lethality, chromatin binding and viral replication" doc

... Access Research Contribution of the C-terminal region within the catalytic core domain of HIV-1 integrase to yeast lethality, chromatin binding and viral replication Zaikun Xu †1 , Yingfeng Zheng †1 , ... mutagenic analyses of the C-terminal region of the catalytic core domain of HIV-1 IN in order to delineate the critical amino...

Ngày tải lên: 13/08/2014, 05:21

... but ultimately tight, binding of PLP to the apoenzyme in vitro and for the fact that preparations of the mutant enzymes are always largely as apoenzyme. Similarly, inflexibility of the protein ... Conversely, the less hydrated environ- ment of the high-pH conformation favors the 340 nm- absorbing enolimine tautomer of the wild-type cofactor and the 388 nm-ab...

Ngày tải lên: 31/03/2014, 08:20

... histologically proved), non-compressive neuropathy confirmed by electromyography, or the diagnosis of Sjögren syndrome based on the clinical symptoms of dry eyes and dry mouth (confirmed by a ... test and/ or kerato- conjunctivitis sicca with involvement of salivary glands docu- mented by positive lip biopsy and/ or salivary scintigraphy). Simple x-rays of the hands and fe...

Ngày tải lên: 09/08/2014, 10:20

... of the protein. The position of lysines in two tri- lysine regions and introduced mutations are shown at the bottom of sequence. B) The expression of the wild-type and mutant IN-YFP fusion proteins ... of lysine residues and therefore, we term them proximal tri-lysine region and distal tri-lysine region, respectively (Fig. 2A). All of these lysine residues ar...

Ngày tải lên: 13/08/2014, 09:21

... d b y loose closure of the dura and skin layers presum a b l y allows for expansion of the edematous brain into a durotomy “bag” under the loosely closed scalp without restriction by the ... meninges. The method using temporal fascia is similar to the one introduced by Yu et al. 32 They separated the temporal deep fascia from the temporal muscle to the...

Ngày tải lên: 25/10/2012, 11:40

... score was thirty-seven. Lifestyle Q1 to Lifestyle Q3 were equally weighted and represented 81% of the total value of the questions, whereas, Lifestyle Q4 and Lifestyle Q5 represented only ... CNS and psychiatric conditions, and then to specify the name of that con- dition. If the participant failed to indicate the name of the condition regardless of a “...

Ngày tải lên: 26/10/2012, 09:57

... composition was very similar to the WSM, and consequently the EDTA-IM. Hydroxyproline, hydroxy- lysine and phosphoserine were not detected in WSM, WIM, EDTA-SM and EDTA-IM. The comparison of the amino ... Proline, hydroxyproline and hydroxylysine were examined at 254 nm by reverse-phase HPLC of their phenylisothiocarbamate derivatives [26], as reported previously [20]....

Ngày tải lên: 21/02/2014, 01:21

... decades of life and severely impair visual acuity in adulthood. Their therapy is restricted to keratopl- asty and phototherapeutic keratectomy by Excimer laser. Unfortunately, the benefits of these therapies ... that Cys–Cys bond formation does facilitate amyloid precipitation, but is not the only factor responsible for the amyloidogenicity of C110)131 peptide. The particip...

Ngày tải lên: 21/02/2014, 01:21

... pathway to b-carot ene proceeds via three enzymatic steps carried out by the enzymes phytoene synthase, phytoene dehydrogenase and lycopene cyclase. The enzyme phytoene dehydrogenase is able to ... both Zygomycetes. In Phycomyces they complement neither the carR nor the carA mutants, and i n Mucor they complement neither the carP nor the carR mutants. They are white, l ack...

Ngày tải lên: 22/02/2014, 04:20

... confirming the heterozygous sta- tus (M/F) of the sample analysed. Experimental strategy designed to analyse the new a s1 -Cas variant at the nucleotide level To determine the coding sequence of a ... material to synthesize cDNA. However, as expected from the phen otypic analysis (at the p rotein level), the f ew animals yielding in their milk the a s1 -CasM variant...

Ngày tải lên: 22/02/2014, 04:20