Báo cáo y học: " Optimization of the doxycycline-dependent simian immunodeficiency virus through in vitro evolution" docx

... thank the Cameroon Ministry of Scientific Research and Innovation, the Ministry of Health and the Ministry of Forestry and Wildlife, which provided the necessary permits for this work. We thank the ... SIVcpz infection resembles that of HIV-1 in humans. Given the poor health status of the animal described in this study, the administration of antiretroviral ther...

Ngày tải lên: 13/08/2014, 01:20

... corresponding tet-operator (tetO) DNA binding sites were inserted into the LTR pro- moter. Since the rtTA protein can only bind tetO and acti- vate transcription in the presence of doxycycline (dox), HIV-rtTA ... repli- cates exclusively in the presence of dox. As the TAR muta- tions and tetO elements were introduced in both the 5' and 3' LTR, they are stably m...

Ngày tải lên: 13/08/2014, 05:20

... the protein without seriously affecting the substrate binding affinity. Binding analyses using BIAcore system To examine whether the mutation of Gly10 to Leu or Pro affects the substrate binding ... models, in which the oxygen atoms are shown in red. The side chain of Tyr170 is also illustrated by a blue stick model. The main chain of Gly10 is shown in red. N, N-termi...

Ngày tải lên: 17/03/2014, 17:20

... but cases of avascular necrosis of the cervical spine following radiotherapy to the larynx appear to be extremely rare. A review of the English language literature has shown only one other case where ... of the cervical spine 20 years after radiotherapy treatment for a T1aN0M0 laryngeal carcinoma resulting in quadriplegia. Conclusions: Radiotherapy carries a long-term risk...

Ngày tải lên: 11/08/2014, 17:21

... pp38 is involved in early cytolytic infection of lymphocytes, but not the induction of tumors [11]. Recently studies showed that the mechanism of attenuation of MDV does not involve the 132 bp repeat ... play an impor- tant role in both protecting against MDV infection and tumor repression in chickens [23,24]. These findings suggest that the Meq gene may be involved in...

Ngày tải lên: 11/08/2014, 21:21

... Doses of 150 μg or higher provide sustained bronchodilation that is largely independent of disease severity. Finally, it is instructive to place the findings of this analysis in the context of the ... each of the comparators included in this analysis. The most striking feature of the plot is that the response to indacaterol at the plateau exceeds the response...

Ngày tải lên: 12/08/2014, 13:22

... alteration of the PNG sequence of gp120 may alter its binding to DC-SIGN, or alternatively the glycosylation machinery of the cell can influence the interaction of the virus with DC-SIGN. To test the ... inhibition of infection of CD4 + T-lym- phocytes. This may be explained by the fact that in our experiments we compensate for the presence of CQ in the...

Ngày tải lên: 13/08/2014, 09:20

... chromosomes induce cortical actin and myosin II assembly into a distinct actincapsurroundedbyamyosinIIringintheMII eggs [6]. Interestingly, sperm chromatin incorporation at fertilization or m icroinjection ... signals. Microinjection of DNA beads revealed sequential assembly of cortical actomyosin cap and myosin II ring We further fo llowed up the kinetics of actin and myosin II assem...

Ngày tải lên: 13/08/2014, 18:21

... surely allow a better understanding of the evolutionary history of a set of genes that control a basic biological function, and also offer interesting insights into the mechanisms of their coordinated expression. ... coordinates the expression of the whole set of energy-producing genes in Drosophila [57]. The comparative analysis of the 78 OXPHOS genes in the t...

Ngày tải lên: 14/08/2014, 14:21

... [31]. RESULTS Erythromycin, Cl-tetracycline and streptomycin inhibit nuclear splicing in vitro The effects of eight different antibiotics (the aminoglycoside, kanamycin, the a minocyclitol glycoside, streptomycin, ... with the binding o f the functional sub strate. Type-II binding sites are the central metal ion-binding pock- ets i n the catalytic cores of ribozymes. In...

Ngày tải lên: 21/02/2014, 15:20