Báo cáo khoa học: " Surfactant for acute respiratory failure in children: where should it fit in our treatment algorithm" doc

... Surfactant was used within the first 24–48 hours of intubation in three trials [7,9,10], and within the first 5 days in another [8]. The timing of surfactant use was not stated in the remaining ... good evidence for the benefit of surfactant in paediatric patients with hypoxic respiratory failure, therefore, many questions remain unanswered – such as the optimum timing of a...

Ngày tải lên: 13/08/2014, 03:21

... (normal). Definition of acute respiratory failure The definition of ARF was based on the SOFA score criteria, in which a score of 3 or 4 is defined as ‘severe’ organ failure [8]. According to those criteria, ... outcome in the six study groups of acute respiratory failure with increasing number of additional organs in failure Mortality (n [%]) Group n Age (years [mean])...

Ngày tải lên: 12/08/2014, 19:22

... of surfactant in children with acute respiratory failure: MortalityMeta-analysis of trials of surfactant in children with acute respiratory failure: Mortality. ALI, acute lung injury; ARDS, acute ... of surfactant in children with acute respiratory failure: Duration of PICU stayMeta-analysis of trials of surfactant in children with acute respiratory fai...

Ngày tải lên: 13/08/2014, 03:21

... patients with underlying condition/Number at-risk with underlying condition in CHR)/(Number of sARF patients without underlying condition/Number at-risk without underlying condition in CHR)). ... completion of our study; thus, we used as our primary case-definition, acute renal failure severe enough, in the opinion of the treating intensivist, to warrant the initiation of RRT....

Ngày tải lên: 12/08/2014, 23:20

... large extracellular C-terminal domain [2]. It is the light subunit that possesses the amino acid transportation activity, although without interacting with the heavy subunit it is unable to reach ... European Bioinformatics Institute’s server (http://www.ebi.ac.uk/) for clu- stalw2 [53] as Phylip-tree type and neighbour-joining clustering [26] using the alignment of complete sequences,...

Ngày tải lên: 18/02/2014, 13:20

... points and stained for F-actin (red). The GFP intensity (represent- ing virus production) increased significantly over time, as seen pictorially in Fig. 3A–E and in graphical form in Fig. 3F. Infection ... FEBS 5281 damine-conjugated phalloidin (Invitrogen) was added for F-actin staining at room temperature for 45 min. Finally, the cells were washed three times with 1· NaCl ⁄ P i b...

Ngày tải lên: 18/02/2014, 14:20

... metal-binding mech- anisms are possible in which the complete multiple metal-binding site forms in preference to partially filled binding sites. Metallothionein (MT) is a metalloprotein found in nearly ... coordination of the first two metal ions to the a domain in independent tetra- thiolate sites, the choice for the third incoming metal ion would be to form a bridging interaction i...

Ngày tải lên: 19/02/2014, 00:20

... proteins are important for inducing protective immunity [52–59]. A synergistic increase in neutralizing antibody titers by Table 1. Vaccines against dengue fever by the Initiative for Vaccine ... NS3 serine protease, the standard inhibitors of serine proteases have been assayed. The serine protease inhibitor, aprotinin, has been shown to inhibit the four CF40GlyNS3pro proteases with hig...

Ngày tải lên: 19/02/2014, 00:20

... N-ter- minal or C-terminal histidine tags. For that purpose, a cod- ing sequence for six histidines was introduced by PCR either at the N-terminus, after the ATG, or at the C-termi- nus before the ... histi- dine-tagged proteins was done as described above. Strains, growth conditions and cell extracts The E. coli strain DH5a was used in the cloning proce- dures. It was grown in LB m...

Ngày tải lên: 19/02/2014, 06:20

... synthesis with cleavable, N-ter- minal signal peptides that almost invariably contain an essential twin-arginine motif in the N-terminal domain [9,10]. They then interact with a translocon in the inner ... substrates initially bind to the TatB and TatC subunits, and it thus appears that these subunits cooperate to form the substrate binding site. In plants, the TatA homolog was only f...

Ngày tải lên: 19/02/2014, 17:20