Báo cáo y học: "HIV infection of thymocytes inhibits IL-7 activity without altering CD127 expression" pot

... Access HIV infection of thymocytes inhibits IL-7 activity without altering CD127 expression Charlene D Young 1,2 and Jonathan B Angel 1,2,3* Abstract Background: Thymic function is altered in HIV infection ... CD4 lymphocytes from HIV-infected patients. Aids 2007, 21:101-103. doi:10.1186/1742-4690-8-72 Cite this article as: Young and Angel: HIV infection of thymocytes...

Ngày tải lên: 13/08/2014, 01:21

... Golay J, Int- rona M: A human immunodeficiency virus type 1 pol gene- derived sequence (cPPT/CTS) increases the efficiency of transduction of human nondividing monocytes and T lym- phocytes by ... primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replica- tion. J Virol 2004, 78:12735-46. 157. Yamashita M, Emerman M: The cell cycle independence of HIV...

Ngày tải lên: 13/08/2014, 09:20

... type 1 virions increases productive infection of CD4+ T lymphocytes by favoring cytosolic deliv- ery of viral material. J Virol 2003, 77:12299-12309. 22. Tardif MR, Tremblay MJ: LFA-1 is a key ... CMV or T7 RNA polymerase promoter. After DNA transfection, the func- tionality of the CD4 proteins was initially evaluated in HeLa cells by a syncytia-forming assay. Infection of trans-...

Ngày tải lên: 13/08/2014, 09:21

... enriched for lymphocytes by Per- coll density gradient centrifugation, as previously described [40,42]. Viability of intestinal lymphocytes averaged 88 ± 4%, as determined by trypan blue exclu- sion. Plasma ... calculated by multiplying the percentage of CD3 + CD4 + T cells by the number of total lymphocytes/ml from complete blood count (CBC) analysis. Further multiplication of the...

Ngày tải lên: 12/08/2014, 23:21

... carbonyl oxygen of the P1 residue, Zn1 is bound by the two carboxyl oxygens of Asp25 5 (Asp347) and a car boxyl ox ygen of Glu334 (Glu429). T he interact ions of Zn1 and the carboxyl oxygen of Asp332 ... cleavage of the Leu-Tyr peptide. For example, the K354R and K354M enzymes retained 14 and 11% wild-type His 6 –LAP-A activity levels, respectively (Table 3). Kinetics of hydro...

Ngày tải lên: 08/03/2014, 23:20

... tetrahydrocolumbamine using this 80% pure tetrahydrocolumbamine. To quantify the enzymatic activity of CoOMT, the transfer of the 3 H-labeled methyl group of S-adenosyl- L - [methyl- 3 H]methionine (0.5 MBqÆlmol )1 )(PerkinElmer Life ... M.H. (1990) S-adenosyl- L -methionine: 3¢-hydroxy-N-methyl-(S) -coclaurine-4¢-O-methyltransferase, a regio- and stereoselective enzyme of the (S)-r...

Ngày tải lên: 17/03/2014, 10:20

... effect of dipyridamole may amplify the anti-inflamma- tory activity of low-dose prednisolone between three- and ten- fold in vivo. The ability of low-dose prednisolone to suppress disease activity ... molecular effects of the antithrombotic agent dipyridamole and a very low dose of the glucocorticoid prednisolone create such an activity profile. Dipyridamole inhibits the ac...

Ngày tải lên: 09/08/2014, 01:22

... to bind to Tyr757 of gp130, Tyr985 of the leptin receptor and Tyr401 of the erythropoietin receptor, Tyr729 of the granulocyte colony-stimulating factor (G-CSF) receptor, Tyr800 of the IL-12 ... immunity IL-6 is a pro-inflammatory cytokine that has a progressive function in many inflammatory diseases, whereas IL-10 is an immunoregulatory cytokine that has potent anti-inflammatory a...

Ngày tải lên: 09/08/2014, 06:23

... clustering of disease susceptibility loci for a number of human autoimmune diseases and animal models of autoimmu- nity [1,2]. Recently convincing proof of this hypothesis has been provided by the ... aim of this study was to fine map the IDDM8 region on chromosome 6q27, focusing on the genes in the region, to identify polymorphisms that may contribute to susceptibility to RA and po...

Ngày tải lên: 09/08/2014, 08:22

... dis- order of unknown etiology. Lymphocytic infiltration of the lach- rymal and salivary glands leads to dry mouth (xerostomia) and dry eyes (xerophthalmia). It has been assumed that a combina- tion of ... instructions (Euroimmun). Preparation of lymphocytes and flow cytometry Lymphocyte suspensions were prepared from spleens and lymph nodes. For flow cytometric analysis, 1 × 10 6 ce...

Ngày tải lên: 09/08/2014, 10:23