Báo cáo y học: "Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines" pot

... system [30, 31] . Finally, the use of the nonhuman protein LMP1 as a molecular adjuvant may actually be advantageous compared to huma n-derived molecular adjuvants such as CD40, for example, by ... with LMP1 and LMP1-CD40 adjuvanted virus. Human inflammatory cytokine quantitation was performed from the culture supernatants by cytometric bead array (CBA). P values are shown fo...

Ngày tải lên: 13/08/2014, 01:20

... load of 1. 4pF on the nodal membrane. The conductance of the fast potassium current was set from an estimate of the membrane area [16 ], which was based on the nodal capacitance in experimental ... [37] and 10 0 channels/µm 2 [38], respec- tively. The ion conductance of the fast potassium current was based on the work of [16 ], who found a fast potas- sium conductance of 15 nS and...

Ngày tải lên: 13/08/2014, 22:22

... thalassemia/mental retardation syndrome; BRG1, SWI/SNF related transcriptional activator; CAF-1p150, chromatin assembly factor -1 p150 subunit; Dnmt 3a/ Dnmt3b, deoxyribonucleic acid (DNA) methyltransferase 3a and 3b; ... Botchan MR: Association of the origin recogni- tion complex with heterochromatin and HP1 in higher eukaryotes. Cell 19 97, 91: 311 -323. 71. Nonaka N, Kitajima T, Yokoba...

Ngày tải lên: 14/08/2014, 16:21

... using BSA as a standard. DNA cleavage assay Routinely, the DNA substrate labelled at its 3¢ end was used in the cleavage assay. The 505-bp DNA fragment was prepared from pQE32 vector (Qiagen). The ... phos- phorylated in the preparation used in the cleavage assay as Ôphosphorylated SF2/ASFÕ. The amount of htopo I and SF2/ASF was quantified by densitometric scanning of SDS-polyacrylamide...

Ngày tải lên: 24/03/2014, 03:21

... to supply many low -molecular- mass substances such as metals, amino acids and fatty acids [42]. Transferrin and albumin noncovalently bind iron and many low -molecular- mass substances, respectively. As ... nmol 1- palmitoyl-2- (13 -hydroperoxy-cis-9,trans -11 -octadecadienoyl)- 3-PtdCho hydroperoxide was added [15 ]. The oxidation of NADPH was followed at 340 nm at 37 °C and activity w...

Ngày tải lên: 31/03/2014, 08:20

... responses as assessed by local surgical sympathetic and parasympathetic denervation. Acta Physiol Pharmacol Latinoam 19 87, 37:305- 319 . 14 . Ramaswamy K, Mathison R, Carter L, Kirk D, Green F, Davison ... histologically, by determina- tion of plasma amylase and lipase activity, and by immunoassays. • In vitro and ex vivo studies on leukocyte migration, adhesion, cell surf ace marker exp...

Ngày tải lên: 11/08/2014, 03:20

... functional relationships. For any given method, there are advantages and disadvan- tages. The number of false positives and false negatives is a key measurement of accuracy. In our case, the ... functional information was available. While this is appropriate for method valida- tion, the disadvantage is that there are problems with annotation due in part to a lack of standardization, w...

Ngày tải lên: 14/08/2014, 08:20

... during 19 90 as a continuous variable showed a clear trend of increase in disability pension risk with increase in absence days/yr. A 10 -day increase in ab- sence days per annum (scale score ranging ... out- come variable. The analysis was performed in three stages: initially, analysis was performed to establish the association between days of sickness absence in 19 90 and dis...

Ngày tải lên: 26/10/2012, 10:03

... for rheumatoid arthritis. J Rheumatol 19 94, 21: 1027 -10 33. 20. Nakashima K, Hagiwara T, Ishigami A, Nagata S, Asaga H, Kuramoto M, Senshu T, Yamada M: Molecular characterization of peptidylarginine ... anti-α-enolase antibody. CAP1, adenyl cyclase-associated protein 1; EF1α, elongation factor 1 . Table 2 Peptides from non-citrullinated α-enolase sequenced by tandem mass spectrom...

Ngày tải lên: 09/08/2014, 07:20

... ST-elevation infarct regeneration) Trial. Circulation 2006, 11 3 :12 87 -12 94, 4. 21. Miyahara Y, Nagaya N, Kataoka M, Yanagawa B, Tanaka K, Hao H, Ishino K, Ishida H, Shimizu T, Kangawa K, Sano S, Okano ... therapies. Tissue Eng 20 01, 7: 211 -228. 15 . Tambara K, Premaratne GU, Sakaguchi G, Kanemitsu N, Lin X, Nakajima H, Sakakibara Y, Kimura Y, Yamamoto M, Tabata Y, Ikeda T, Komeda...

Ngày tải lên: 10/08/2014, 09:21