Báo cáo y học: "Mutagenesis of tyrosine and di-leucine motifs in the HIV-1 envelope cytoplasmic domain results in a loss of Env-mediated fusion and infectivi" pps

... and di-leucine motifs in the HIV-1 envelope cytoplasmic domain results in a loss of Env-mediated fusion and infectivity Sushma J Bhakta † , Liang Shang † , Jessica L Prince, Daniel T Claiborne and ... create mutant E. Introduction of the Y7 12C mutation to WT and the Env mutants A, B, C, D, and E resulted in the generation of the Y, YA,...

Ngày tải lên: 13/08/2014, 01:20

... not have tryptophans in the aromatic domain, are unable to support entry of SARSpp, yet are capable of initializing membrane fusion, i.e. capable of forming a fusion pore. The aromatic domain of ... of a lysine adjacent to the glycine, was not. In a model, we propose that during fusion, the lateral flexibility of the transmembrane domain plays a crit...

Ngày tải lên: 12/08/2014, 04:21

... ATATAGGCGGCCGCGTGCCACTATG- CACAG Nm103-AP: ATATAGGCGGCCGCCTGAGACTGT- GCATAG Nm103-SP: ATATAGGCGGCCGCCTGCAAATAT- TCTCAC Nm113-AP: ATATAGGCGGCCGCAACATCGT- GAGAATA Nm113-SP: ATATAGGCGGCCGCACAGAACTTC- CAGAT Nm123-AP: ... ATATAGGCGGCCGCGACGCGGGCTC- GAGTA Nm73-SP: ATATAGGCGGCCGCGAAGTACTGC- CAGAGA Nm83-AP: ATATAGGCGGCCGCGCAGGGTCTCT- GGCAG Nm83-SP: ATATAGGCGGCCGCGCACCTCTG- CAAGCTT Nm93-AP: ATATAGGCGG...

Ngày tải lên: 12/08/2014, 23:23

... Germany. They represented a convenience sample of patients in that each was already scheduled for coronary angiography for any indication and had no history of a coronary revascularization ... this database have been validated against the final medical diagnosis of at least two independent expert diagnosticians in the field, including results of angiography and...

Ngày tải lên: 08/08/2014, 16:23

... Asia, and North America on individuals of varying ages and degrees of disease state including normal populations [20,21]. All ECG ana lyses in this database have been validated against the ... before the data analysis was carried out. The quality of the tracing was visually rechecked and graded as “good,” “marginal,” or “poor.” A poor tracing was defined by one...

Ngày tải lên: 08/08/2014, 17:20

... 5’-CGGGATCCATCGAAGGTCGTGAAGATTCGAT GGACAT-3’, and 5’-CGCGCGACCGAGCGGAA GCTTCTATTTTCTTAAAGAGAC-3’. Underlined nucleotides represent the BamH I and Hind III site, respectively. PCR conditions included ... before analyzed by SDS-PAGE and quantitated by using the BCA Protein Assay Kit (Be- yotime, Shanghai, China). 2.4 Immunizations Five-week old female BALB/c mice initially re-...

Ngày tải lên: 08/08/2014, 18:21

... citation purposes) are not true orthologs in that the rodent enzymes can hyperedit both RNA and DNA, unlike hA1 that can only deaminate ssDNA [13]. By contrast AICDA and the human APOBEC3 (hA3) ... editing of HBV DNA by monodomain human APOBEC3 cytidine deaminases and the recombinant nature of APOBEC3G. PLoS ONE 2009, 4:e4277. 29. Noguchi C, Ishino H, Tsuge M, Fujimoto Y, I...

Ngày tải lên: 12/08/2014, 23:22

... ≥ 3-fold the OD of the animal's pre-study sample. Statistical analyses Flow phenotyping results were analyzed using analysis of covariance for PBMC and multivariate analysis of vari- ance for ... diversity, 22 female (Liberty Labora- tory) and 6 male (Harlan Laboratories) SPF cats were obtained, housed, acclimated, and cared for in accor- dance with the standards o...

Ngày tải lên: 13/08/2014, 01:20

... is a Java application that checks state transitions for the agents and any accompa- nying changes in internal variable values. This process was previously done by manu- ally reading the BIS agent ... BB and MK designed and created specialized software for data analysis including and in addition to the AgentVerifier. CE, JD and LD were involved in drafting parts of t...

Ngày tải lên: 13/08/2014, 16:20

... Neverthe- less, using this arbitrary presumption that DNA synthesis may occur only days after inoculation and that it is the G 2 phase rather than G 1 that accounts for much of the delay in physical ... if variations in TMKmyc during the S phase of the tubercle bacillus cell cycle are independent of the time taken by the tubercle in other stages of the cycle, th...

Ngày tải lên: 13/08/2014, 16:21