Báo cáo y học: "WFDC1 expression identifies memory CD4 Tlymphocytes rendered vulnerable to cell-cell HIV-1 transfer by promoting intercellular adhesive junctions" pptx
Báo cáo y học: "WFDC1 expression identifies memory CD4 Tlymphocytes rendered vulnerable to cell-cell HIV-1 transfer by promoting intercellular adhesive junctions" pptx
... al.: WFDC1 expression identifies memory CD4 T- lymphocytes rendered vulnerable to cell-cell HIV-1 transfer by promoting intercellular adhesive junctions. Retrovirology 2011 8:29. Submit your next ... Retrovirology 2011, 8:29 http://www.retrovirology.com/content/8/1/29 Page 13 of 14 RESEARC H Open Access WFDC1 expression identifies memory CD4 T- lymphocytes...
Ngày tải lên: 13/08/2014, 01:20
Báo cáo y học: "Stable expression of a recombinant human antinucleosome antibody to investigate relationships between antibody sequence, binding properties, and pathogenicity" doc
... used to construct the supervectors by combining the light chain and heavy chain expression vectorsCloning method used to construct the supervectors by combining the light chain and heavy chain expression ... cases, expression was achieved using heavy chain-loss variants, which are hybridoma cells that have lost the ability to secrete heavy chains. By transfecting such varia...
Ngày tải lên: 09/08/2014, 06:23
Báo cáo y học: "Gene expression profiling in the synovium identifies a predictive signature of absence of response to adalimumab therapy in rheumatoid arthritis" doc
... Unfortunately, they did not perform any confirmatory experiment (real-time PCR or immunohistochemistry) in order to verify the reality of their microarray data [30]. Their results were also potentially ... wanted to increase the validity of such microarray observations by performing additional RT-PCR and immunohistochemistry experiments and by linking our data to potential mechanism...
Ngày tải lên: 09/08/2014, 14:20
Báo cáo y học: " MicroRNA expression profiling of human breast cancer identifies new markers of tumor subtype" ppt
... that bead-based flow cytometric miRNA expression profiling might be a suitable platform to classify breast cancer into prognostic molecular subtypes. Published: 8 October 2007 Genome Biology 2007, 8:R214 ... using native polyacrylamide gels, ensuring that only mature miR- NAs were assayed. Using this miRNA expression platform we analyzed a total of 137 samples in 168 assays. Assays for...
Ngày tải lên: 14/08/2014, 08:20
Báo cáo y học: "ene expression analysis of human red blood cells"
... human erythrocyte proteome: analysis by ion trap mass spectrometry. Mollecular und Cellular Proteomics 2004;3:501-509. 9. Tyan YC, Jong SB, Liao JD et al. Proteomic profiling of eryth- rocyte proteins ... instance RNA polymerises I,II,III, zinc/PHD finger- DNA-binding proteins, cysteinyl, lysyl-tRNA syn- thetase), important RNA-stabilising factor - poly(A) binding protein, anti-apopto...
Ngày tải lên: 03/11/2012, 10:58
Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... the cystatin F produced by U937 cells is retained intracellularly or is taken up by the cells immedi- ately following secretion. Accordingly, the relatively low amount of cystatin F produced by ... the diglycosylated cystatin F species. The nature of this band is presently unknown and is discussed below. In order to study the production of cystatin F compared to the ubiquitous inhib...
Ngày tải lên: 21/02/2014, 01:21
Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... broad speci®city w ith respect to the g lycone moiety of substrates. Human CBG hydrolyses synthetic aryl glycosides (including 4-nitrophenyl and 4-methylumbelliferyl monoglycosides) [9], but no physiological ... 4NP-b- D -mannopyranoside (10 m M )]. Hydrolysis of xenobiotic glycosides by reCBG The a bility of CBG to hydrolyse a variety of glucosides was assessed using a wide variety of...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc
... the total polyunsaturated fatty acids [9]. As polyunsat- urated fatty acids are vulnerable to peroxidation by ROS, and peroxidized lipids and carbonyl compounds produced by this reaction are toxic ... [28] and then transferred onto Hybond-P (Amersham Pharmacia, Pscataway, NJ, U SA) under semi-dry conditions by means of a Transfer- blot SD Semi-dry transfer cell ( Bio-Rad, Tokyo,...
Ngày tải lên: 17/03/2014, 17:20
Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx
... removed completely. The pellet was broken by gently tapping on the bottom of the tube and placed in a 37 °C water-bath. Prewarmed 0.5 mL of 50% poly(ethylene glycol) 1500 (Boehringer Mannheim, Germany) was ... vectors where each gene are controlled by internal ribosome entry site (IRES) [9–11], panel of vectors [12] and multiple episomal vectors [13]. We chose secretory IgA (SIgA) as a mo...
Ngày tải lên: 18/03/2014, 01:20
Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt
... still uncertainty about the amino acid at position 21 encoded by a TAG codon, that may specify glutamine or tryptophan. Therefore, in order to subclone into the expression vector pET11a (NdeI/BamHI ... region) was automatically sequenced on an ABI Prism sequence analyzer Model 373A (PE Applied Biosystems) using the Big Dye Terminator Methodology (PE Applied Biosystems), to verify the...
Ngày tải lên: 23/03/2014, 21:20