Báo cáo y học: "Antiviral activity of a-helical stapled peptides designed from the HIV-1 capsid dimerization domain" pps

... of Medical Virology and Epidemiology of Virus Diseases of the Hygiene Institute at University of Tübingen, respectively. For IFN assays HeLa cells (University of Jena, Germany) were incubated with ... Gripp-Heel® inhibited the replication of a variety of respiratory viruses. Additionally, we showed that pre-incubation affects the magnitude of the inhibitory effect diff...

Ngày tải lên: 11/08/2014, 08:21

... in core assembly being reversible by VSV-G pseudotyping [75]. Anti -HIV-1 activity and cytotoxicity of stapled peptides in cell-based assays We measured the anti -HIV-1 activity of NYAD-201 and its ... Montelaro (University of Pittsburgh). Synthesis of stapled peptides The peptides were synthesized manually by Fmoc solid phase synthesis as described previously [59]...

Ngày tải lên: 13/08/2014, 01:20

... BSA as the standard. Enzymatic assay of phosphatase activity The phosphatase activity was measured by hydrolysis of 10 m M p-nitrophenyl phosphate as a substrate in 100 m M Mes (pH 6.0). The reaction ... m M H 2 O 2 on a Cary 50 [23]. The kinetic parameters were determined using the ENZYMEKINETICS program from Trinity Software. Assay of PhoN-Se brominating activity. Th...

Ngày tải lên: 24/03/2014, 03:21

... freezing. During the winter they also increase the concentration of the cryoprotectant, glycerol, 10-fold and desiccate to 40% of the prediapause water content [4]. Taken together, these adaptations may explain ... Denhardt's solution for a minimum of 12 h [19]. To examine the complexity of the gene family, the blot was hybridized with the 337 isoform probe and wa...

Ngày tải lên: 21/02/2014, 15:20

... patients who choose this type of therapy include limitations due to the cost of repeated endoscopies, palatability of elimination/elemental diets and the availability of subspecialists trained ... eosinophil/HPF in the distal esophagus. Corn and eggs were introduced and stopped because they caused symptoms to return. Three weeks after the reintroduction of cow’s milk, wheat...

Ngày tải lên: 08/08/2014, 21:20

... suppressor activity. The PR domain of RIZ1 possessed anticancer activity from the transfec- tion studies. This finding leaves us an important question on the roles the other structural components of ... anticancer activity. In this study, we quan- titatively analyzed the mRNA expression level of RIZ1 over the disease progression in eight different types of cancer...

Ngày tải lên: 25/10/2012, 11:15

... related cytopenias. The use of these agents is costly and has not been studied rigorously in a randomized controlled format to assess their value and impact on efficacy of therapy although they are ... with the latest combination therapy. However, existing data support therapy of the patient with compensated cirrhosis. Cirrhotic patients may tolerate therapy less well, given...

Ngày tải lên: 31/10/2012, 17:11

... systematic analysis of the role played by the two sets of Ser/Thr residues in the virus-dependent activation of IRF-3. To this end, we examined the phenotypes of a variety of hIRF-3 mutants by ... none of the IRF-3 constructs activated the reporter in the absence of mCBP, suggesting that IRF-3 by itself is devoid of intrinsic transcriptional activity. Fig. 2....

Ngày tải lên: 17/03/2014, 10:20

... amidolytic activity was obtained by showing that commercial PAP hydrolysed the Abz-ISYQSSSTEEQ- EDDnp substrate at neutral pH, as does the puri®ed enzyme. The ratio of the amidolytic activity of ... at the elution time of PSA was observed; (d) commercial PAP has amidolytic activity similar to that of puri®ed PAP. The fact that there was a shift in the cleavage of...

Ngày tải lên: 24/03/2014, 00:21

... days 3, 7, 10, 14 and 17. Results Anti -HIV-1 activity and cytotoxicity of WR1065 in human TCBs In HIV-1- infected human TCBs, the HIV-1 titers, deter- mined in the absence of drug, ranged from ... treated the cells and performed the cytotoxicity assays and immunoassays for virus titer. OAO provided important conceptual input regarding the cyto- toxicity assays. GF prov...

Ngày tải lên: 10/08/2014, 05:21