Báo cáo y học: "Analysis of XMRV integration sites from human prostate cancer tissues suggests PCR contamination rather than genuine human infection" ppt

... OND E N C E Open Access Analysis of XMRV integration sites from human prostate cancer tissues suggests PCR contamination rather than genuine human infection Jeremy A Garson 1 , Paul Kellam 1,2 , ... authenticity of the XMRV integration sites that have been reported to date. Theonlyresearchgroupdescribing patient-derived XMRV integration sites prov...

Ngày tải lên: 13/08/2014, 01:20

... Netherlands. 2 Department of Microbiology, Virology Unit, Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium. 3 Department of Clinical Chemistry, Microbiology and Immunology, Faculty of Medicine ... clones are largely deficient in replicative capacity. LTR promoter activity The promoter activity of the LTR region of the primary and superinfecting HIV stra...

Ngày tải lên: 13/08/2014, 01:20

... that may clarify the origin of duplicated tran- scripts in the genome assembly. BAC assembly The quality of these BAC assemblies is critical for a valid assessment of the genome assembly and the ... R88 Research Analysis of 14 BAC sequences from the Aedes aegypti genome: a benchmark for genome annotation and assembly Neil F Lobo ¤ * , Kathy S Campbell ¤ † , Daniel Thaner * , Becky...

Ngày tải lên: 14/08/2014, 07:21

... minority of the patients had previously been typed with a lymphocytotoxicity test or by restriction fragment length polymorphism as described before [2]. C4A gene deletion was determined by PCR ... previously described [20]. Analysis of Fc γ RIIIa gene polymorphism The analysis of the F/V polymorphism was performed essentially as previously described [21]. MBL gene polymorphism Varian...

Ngày tải lên: 09/08/2014, 01:24

... (white) of each codon of productively rearranged V L genes of B cells isolated from peripheral blood and from the parotid gland of a patient with Sjögren’s syndrome. The frequency of mutation of each ... cells obtained by minor salivary gland biopsies from two patients with SS [6]. Detailed analyses of the frequency of occurrence and mutations of the highly mutable mot...

Ngày tải lên: 09/08/2014, 03:24

... the number of glycosylation sites, and neutrophils from individuals homozygous for the FCGR3B-NA2 allele have been found consistently to exhibit lower levels of phagocytosis than FCGR3B-NA1 homozygotes ... ultimately be required. The FcγRs play important roles in the initiation and propaga- tion of many different immunological and inflammatory proc- esses. Consequently, they may ac...

Ngày tải lên: 09/08/2014, 07:20

... canine OA and from the results of a preliminary canine- specific whole genome microarray study, using a small number of samples. Assays were designed for quantification of expres- sion of five collagen ... followed by 40 cycles of 95°C for 1 minute and 60°C for 15 seconds, as recommended by the manufacturer (Applied Biosystems). Real-time data were ana- lysed by using the Sequence...

Ngày tải lên: 09/08/2014, 08:22

... University of Pavia, Via Taramelli 5, 27100 Pavia, Italy 2 Department of Biochemistry, University of Pavia, Via Taramelli 5, 27100 Pavia, Italy 3 Department of Internal Medicine, University of Pavia, ... 2, the upregulation of Cal B, cytohesin-2, and calumenin in BALF from SSc Fib+ patients could further promote the synthesis of pro-inflammatory cytokines, the influx of inf...

Ngày tải lên: 09/08/2014, 08:22

... phenotypic analysis of lymphoid populations was per- formed. As expected, all MRL mice had expanded lymphocyte populations, primarily in CD4 - CD8 - double-negative T cells. By flow cytometry, ... loss of the major negative regulator of the classical complement pathway fails to impact disease severity, the use of the MRL mouse as a prototypical model of immune complex renal injury...

Ngày tải lên: 09/08/2014, 10:21

... use of the T4 Gene 32 Protein, which may increase the yield of PCR products [30- 32]. Sequence analysis of the PCR- positive samples revealed the presence of a mixture of bacterial DNA in synovial ... determined by microimmunofluorescence assay. b Chlamydia PCR in genital swabs was determined by Cobas Amplicor PCR assay (Roche Diagnostics Molecular Systems, Inc, CA, USA). c...

Ngày tải lên: 09/08/2014, 10:23