Báo cáo y học: "Distinct host cell proteins incorporated by SIV replicating in CD4+ T Cells from natural disease resistant versus non-natural disease susceptible hosts" docx

... 7:107 http://www.retrovirology.com/content/7/1/107 Page 2 of 15 RESEARC H Open Access Distinct host cell proteins incorporated by SIV replicating in CD4 + T Cells from natural disease resistant versus ... generated by replication within primary CD4 + T cells from disease susceptible RM and disease resistant SM. Data presented herein document the i...

Ngày tải lên: 13/08/2014, 01:20

... suggest that CREB may have a dual role in cytokine expression in CD4 T cells by directly blocking the IFN-γ promoter and by indirectly regulating T cell differentiation or cytokine gene transcription ... Phosphorylation of TTP by MK2 has been shown to induce the binding of 14-3-3 proteins to TTP, thereby withholding TTP from the mRNA degradation machinery [97,98]. Howeve...

Ngày tải lên: 09/08/2014, 07:20

... compared its activity to the activity of Exon2-luc in transient transfection assays. The mutation led to complete loss of activity in these assays, indicating that the integrity of the AUG is ... 5¢-GGGGATCCATGCCATCAAAAATAAGGA TTAAT-3¢ SG142: 5¢-CCTCTAGATTAATCCTTATTTTTGATGG CCCCCTTCTGTCTCTGCCTCCCAAGGACTGGC-3¢ SG35: 5¢-CGGAATTCCCCGTGCTGGAGGCGACT CG-3¢ SG122: 5¢-CGGACGGCGCGGAGAC 3¢ b-actin...

Ngày tải lên: 08/03/2014, 09:20

... during acute SIV- mac infection in MACs but interestingly not during acute SIVagm infection in AGMs. This may be relevant for HIV infection, where the intestinal mucosal system is an early major ... unclear. The study of acute SIV infections in non- human primate models contributes to the understanding of the early virus /host interactions. SIVmac infection in macaques (MACs) bes...

Ngày tải lên: 13/08/2014, 09:20

... activity than uninfected cells. To verify that the observed increase in cyclin E/cdk2 kinase activity in HTLV-1 infected cells was not limited to one cell line, another set of T- cells was examined. ... also has the abil- ity to increase cdk4 associated kinase activity in HTLV-1 infected cells by binding to p16/INK4A, resulting in the inability of p16/INK4A to effectively...

Ngày tải lên: 13/08/2014, 13:20

... are interesting in this context. Although a detailed study of the cystatin F transport route was beyond the scope of the present investigation, the fact that cystatin F is present in significant ... inhibitor, cystatin C, we measured the cystatin contents in cell lysates and culture media of cells grown for 2 and 4 days with cystatin F and cystatin C specific ELISAs. Generally, the to...

Ngày tải lên: 21/02/2014, 01:21

... subsequent joint destruction in RA. To investigate cell- contact-mediated activation of monocytes by preactivated T cells, monocytic cell lines or monocytes from healthy donors have been primarily used ... previously been shown to exhibit a pronounced inhib- itory activity. To analyse the influence of clinical parameters of disease activity on the inhibitory activity exhibited...

Ngày tải lên: 09/08/2014, 07:20

... suggests the possibility that the observed gene altera- tions are the effect of a cell adaptation to a particularly hostile environment. It is interesting to underline the induction of 3 metallothionein ... indicate down-regulation of PLXNB1, a receptor for sem- aphorin that tracks the pathway to migrating endothelial cells by activation of the MET oncogene and by stimulating Rho-ini-...

Ngày tải lên: 09/08/2014, 08:22

... with a different virus strain indicate that this cell subset may easily facilitate infec- tion by different variants. Although strain I was not detected in serum during therapy, its presence in ... report on preferential HIV-1 i nhibit ion during AZT treatment in activated cells over slowly dividing cells in vitro,may indicate that the vast majority of virus in the circulati...

Ngày tải lên: 10/08/2014, 05:21

... roller bottles. The cells were matured over two days, harvested and analyzed for cell yield and mature DC phenotype by flow cytometry, and then functionally analyzed for their ability to activate allogeneic ... that would destroy the product. In our initial experiments, we found that monocytes had a similar capacity to adhere to the plastic inside the roller bottles as in the stati...

Ngày tải lên: 11/08/2014, 10:23