Báo cáo y học: " Membrane topology analysis of HIV-1 envelope glycoprotein gp41" pps

... Raviv Y, Blumenthal R: Photoinduced reactivity of the HIV-1 envelope glycoprotein with a membrane- embedded probe reveals insertion of portions of the HIV-1 Gp41 cytoplasmic tail into the viral membrane. ... [18-24]. Therefore analysis of the topology and structur es of the transmembrane domain of gp41 is critical for our understanding of the mechanism of the m...

Ngày tải lên: 13/08/2014, 01:20

... an analysis of Drosophila alcohol dehydrogenase genes [54], we estimate that Tc-proPO2 and Tc-proPO3 arose by gene dupli- cation approximately 0.6 million years ago. The phylogenetic analysis ... in1 attacin2 Toll2 cSPH2 Spz1 cSP66 Toll3 Toll4 TEP-C proPO1 cecropin3 PGRP-SB Toll1 galectin2 serpin29 PGRP-SA defensin3 defensin4 CTL7 imd cSP136 serpin30 lysozyme2 lysozyme3 β GRP3 β GRP1 ly...

Ngày tải lên: 14/08/2014, 08:20

... which can display various plots of statistical analysis for quality control. Background High-density oligonucleotide tiling-microarrays currently provide the most powerful method of investigating ... log−intensity Log−intensity Frequency 67891011 0 5,000 15,000 25,000 All probes GC <20 46810 0.0 0.1 0.2 0.3 0.4 0.5 0.6 (b) Channel bias in log−intensity Log−intensity Density IP/Cy3 In...

Ngày tải lên: 14/08/2014, 08:20

... echocardiography allowing a three-myocardial layer-specific analysis of deformation pa- rameters. Eur J Echocardiogr 2009; 10(2): 303-308. 9. Becker M, Hoffmann R, Kuhl HP, et al. Analysis of myocardial ... strain analysis of the global and regional myocardial function for the differentiation of pathologic and physiologic left ventricular hypertrophy: A study in athletes and...

Ngày tải lên: 25/10/2012, 11:15

... (Affymetrix). DNA microarray analysis Data analyses were performed using the GeneChip Expression Analysis Software (version 3.2, Affymetrix). First, single array analysis was performed [11] ... One-Cycle cDNA Synthesis Kit (Affymetrix). Fifteen micrograms of labeled and fragmented cRNA were subsequently hybridized to a Human Genome U133 plus 2.0 microarray (Affymetrix). After...

Ngày tải lên: 31/10/2012, 15:28

... qualitatively by immunoblot analysis of sera from patients of both the group. Figure 2 shows the IgG immunoblot profile of the sera of patients with deep-seated and superficial infections respectively. ... and healthy control group. These two unique bands are likely to be of diagnostic value. The diagnostic efficacy of antibody detection tests was not the primary objective...

Ngày tải lên: 02/11/2012, 11:08

... erythrocyte proteome: analysis by ion trap mass spectrometry. Mollecular und Cellular Proteomics 2004;3:501-509. 9. Tyan YC, Jong SB, Liao JD et al. Proteomic profiling of eryth- rocyte proteins by ... contain typical eu- karyotic RNA with 28S- and18S-rRNA standard bands. Microarray studies revealed the presence of transcripts of 1019 different genes in erythrocytic RNA. Gene Onto...

Ngày tải lên: 03/11/2012, 10:58

... into 1 mL of the assay mixture. Determination of ATP hydrolyzing activity The coupled enzyme assay was used to determine ATP hydrolyzing activity of the different samples [30]. Preparation of lipid ... dimroth@micro.biol.ethz.ch Abbreviations: DCCD, dicyclohexylcarbodiimide; POPC, 1-palmitoyl- 2-oleyl-sn-glycero-3-phosphocholine; ACMA, 9-amino-6-chloro- 2-methoxyacridine; SLPC, 1-palm...

Ngày tải lên: 08/03/2014, 09:20

... tissue. The use of a cellular standard generated with activated PBMC cDNA significantly improves assay reliability by reducing variation and by simplifying assay development. Analysis of RA synovia indicates ... variation in PCR assays is slightly greater than that required for a clinical laboratory analysis [26], the precision is sufficient to allow detection of twofold differences...

Ngày tải lên: 09/08/2014, 01:23

... this study. Pregnancy outcome: statistical analyses The majority of the analyses described here are pregnancy based. Women were excluded from this analysis if they were aged younger than 18 years ... analyses at the time of interview. Ethical approval was granted by the Ethics Committee of the London School of Hygiene & Tropical Medicine and by the Ministry of Health of the...

Ngày tải lên: 09/08/2014, 10:22