Báo cáo y học: "Polymorphisms of HIV-2 integrase and selection of resistance to raltegravir" potx
Báo cáo y học: "Metabolic cycle, cell cycle, and the finishing kick to Start" potx
... rate of respiration is shown by a high rate of oxygen consumption,whereas a high rate of glycolysis is shown by a respiratoryquotient that spikes above 1, and by the loss of storage carbo-hydrate ... the top of the spike; and budding index varies from 5% to 40%. G1 S(a)Cyclin mRNABudding of cellsGlycogen and trehalosecAMPGlycogen and trehaloseEthanolDissolvedoxygenRespiratoryquotientBudding ... researchOpinionMetabolic cycle, cell cycle, and the finishing kick to StartBruce FutcherAddress: Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794, USA.Email:...
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Báo cáo y học: "Pravastatin Provides Antioxidant Activity and Protection of Erythrocytes Loaded Primaqe"
... Involvement of reactive oxygen species (ROS) has been demonstrated in the toxicity of many drugs to erythrocytes [7]. PQ is used for treatment of malarial infections and its loading to erythrocytes ... erythrocytes. The erythrocytes were classified into; control erythrocytes, erythrocytes incubated with either 2 mM of PS or 2 mM of PQ, and erythrocytes incubated with combination of PS plus ... 001). Table 1: Hemolysis profile of control erythrocytes and erythrocytes exposed to either PRV or PQ at concentra-tion 2 m mole /L NaCl g/L % of erythrocytes hemolysis Control PS PQ PS+...
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Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
... 7.4,5mMazide inhibited ADP hydrolysis by 70% whereasATP hydrolysis was inhibited by only 10%. Inhibition of ADP and ATP hydrolysis by azide was greater when the pH of assay solutions was 6.4. Azide ... ecto-ATP-diphosphohydrolases (ecto-ATPDases orecto-apyrases) hydrolyze NDPs as well as NTPs and areinhibited by high concentrations of azide, whereas the ecto-ATPases show little activity toward ... 0.2 and 0.64 depending on the pH and the presence of NP-40; (d)Fig. 3. Inhibition of ATP and ADP hydrolysis activities of purifiedchicken liver ecto-ATPDase by azide. ATP and ADP hydrolysis...
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Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx
... (GLUT4) and could therefore play a role in thefusion of these vesicles with the plasma membrane [45].Binding of guanine nucleotides and hydrolysis of GTPby GST±Rab31 and GST±Rab32Many studies ... lg of platelet protein and 0.5 lg of GST and GST±Rab p roteins; (B) an[a-32P]GTP overlay [17] of a nitrocellulose blot of a gel containing40 lg of platelet protein, 0.5 lgofGSTand0.5lgofGST±Rabproteins, ... only Rab31 and not GST, GST±Rab32 orGST±Rab5A (Fig. 7C). Similarly, antibody to Rab32detected only Rab32 (and minor proteolytic fragments)(Fig. 7D).[35S]GTP[S] binding by GST±Rab31 and...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
... Supramolecularreceptor structures in the plasma memb rane of lymphocytesrevealed by flow cytometric energy transfer, scanning force- and transmission electron-microscopic analyses. Cytometry 33,225–233.23. ... Simons, K. (1999) Clusters of glycolipid and glycosylphosphatidyl-inositol-anchored proteins in lymphoid cells:accumulation of actin regulated by local tyrosine phosphorylation.Eur. J. Immunol. ... chain of class I HLA was used fordetection (data not shown).Then we analyzed plasma membrane topography of IL-2R subunits and HLA molecules by both flow cytometric[17–19] and microscopic photobleaching...
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Báo cáo y học: "Biomarker profiles in serum and saliva of experimental Sjögren''''s syndrome: associations with specific autoimmune manifestations" ppt
... University of Bergen, Jonas Liesvei, Bergen 5021, Norway3Department of Pathology, Haukeland University Hospital, Jonas Liesvei, Bergen 5021, Norway4Department of Pathology, Immunology and Laboratory ... salivary glands. The presence and local production of some inflammatory mediators and autoantibodies in the sal-ivary glands were previously described (Additional file 1 [Sup-plementary table ... findings any SS-unre-lated impact of hyperglycaemia on the physiological process of saliva secretion and the anticipated distorting effect of hyperglycaemia on biomarker profiles.Measurement of stimulated...
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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
... patients may have beenasymptomatic, or the preceding gastrointestinal symptomsmay have been mild and overlooked by the patients [3]. It ispossible that enteric organisms may migrate from asympto-matic ... assay as described by Wang and Grayston [29]. bSerology was determined for Ct-IgA antibodies by ELISA (Labsystems, Hilsinki, Finland). cChlamydia PCR in genital swabs was determined by Cobas ... sequenced. A summary of the total number of sequences analyzed in each patient is depicted in Table 2.Only full-length (1300 to 1400 bp) sequences of high qualitywere analyzed in detail. Most...
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Báo cáo y học: "MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays" pptx
... spectrometry and preprocessed withappropriate tools. MethMarker can directly import DNAmethylation profiles from files generated with BiQ Analyzer[20], QUMA [21] and EpiTYPER [22], and it is easy to ... assays in order to maximize theclassification accuracy of the candidate biomarker. Meth-Marker benchmarks the candidate biomarkers in terms of accuracy, correlation, specificity and sensitivity. ... Biology 2009, 10:R105samples would turn out to belong to the methylated cases or to the unmethylated controls, respectively. Hence, a relativelylarge number of samples were subjected to clonal...
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Báo cáo y học: "Quake: quality-aware detection and correction of sequencing errors" docx
... significantly by quality value. While mis-calls at adenines were highly likely to be cytosines at lowquality, errors were closer to uniform at high qualitypositions in the read. Finally, error ... small repeats. Assembly of the raw uncorrected reads was quite poor because of the very low quality suffixe s of many of the 124 bp reads.Thus, we compare assembly of quality trimmed reads(performed ... AdvancedComputer Studies, and Department of Computer Science, University of Maryland, College Park, MD 20742, USA.2Simons Center for QuantitativeBiology, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold...
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Báo cáo y học: "Comparative genomics reveals birth and death of fragile regions in mammalian evolutio" pdf
... proportional to |C|/ = 2 - 1, that is, the number of 2-breaks required to transform such a cycle into acollection of trivial cycles (| C|standsforthelengthofC). Then we uniformly randomly select ... by a single branch like M+ and D+ separated by MR+), and yellow (for branches that are separated by two branches like M+ and H+ separated by MR+ and QH+).Alekseyev and Pevzner Genome Biology ... (Figure 2c) to thecase of circular chromosomes. We say that a 2-break onedges (u, x), (υ, y) uses vertices u, x, υ and y. Let P and Q be ‘black’ and ‘red’ genomes on the sameset of synteny blocks...
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