Báo cáo y học: " GPI-anchored single chain Fv - an effective way to capture transiently-exposed neutralization epitopes on HIV-1 envelope spike" pot

... 2002 A single charged surface residue modifies the activity of ikitoxin, a beta-type Na + channel toxin from Parabuthus transvaalicus A. Bora Inceoglu 1, *, Yuki Hayashida 2 , Jozsef Lango 3 , Andrew ... Laboratory, University of California, Davis, CA, USA We previously purified and characterized a peptide toxin, birtoxin, from the South African sco...

Ngày tải lên: 31/03/2014, 08:20

... primer). Results and discussion In marked contrast to previous findings, no polymorphic alleles (neither thymine at position 40 nor cytosine at position 1,400) were detected in any of the individuals tested. ... sur- prising because both positions were highly polymorphic in the original publications. In those publications, heterozygosity at position 40 (V14M) was detected in four in...

Ngày tải lên: 09/08/2014, 07:20

... Hematology & Oncology 2011, 4:7 http://www.jhoonline.org/content/4/1/7 Page 2 of 7 RESEARC H Open Access A single-tube allele specific-polymerase chain reaction to detect T315I resistant mutation ... early as possible to detect the lowest levels of the mutant clone [24,25]. In this study, we set out to develop a single-tube allele specific-polymerase ch...

Ngày tải lên: 10/08/2014, 21:23

... Miyamoto D, Hidari KI, Guo CT, Ito T, Kawaoka Y, Suzuki Y: Human trachea primary epithelial cells express both sialyl(al pha2-3)Gal receptor for human parainfluenza virus type 1 and avian influenza ... human CypA, chCypA was iso- lated and characterized and the relevance of chCypA to influenza virus infection was revealed. The discovery that chCypA inhibited influenza virus re...

Ngày tải lên: 11/08/2014, 21:21

... as: Chang et al.: A single dose of DNA vaccine based on conserved H5N1 subtype proteins provides protection against lethal H5N1 challenge in mice pre-exposed to H1N1 influenza virus. Virology Journal ... of DNA vaccine based on conserved H5N1 subtype proteins provides protection against lethal H5N1 challenge in m...

Ngày tải lên: 12/08/2014, 04:20

... systemic lupus erythematosus in Japanese women: a case-control association study Aya Kawasaki 1 , Hiroshi Furukawa 2 , Yuya Kondo 3 , Satoshi Ito 3,4 , Taichi Hayashi 3 , Makio Kusaoi 5 , Isao ... proinflammatory cyto- kines. IRF5 is activated by T LR7 signaling and regulates the expression of many genes, including type I IFN and proinflammatory cytokines [19]. STAT4...

Ngày tải lên: 12/08/2014, 15:22

... emphasis away from linkage analysis and microsatellite markers towards SNP genotyping and different analytical strategies based on association and haplotype analysis [31–34]. Association analyses ... pharmaceutical industry [4]. Asthma is a genetically complex disease that is associated with the familial syndrome of atopy and increased levels of total serum IgE [5,6]. Asthma and atopy...

Ngày tải lên: 12/08/2014, 18:20

... addition to the work by Vanden- broucke and colleagues, three further studies by other investigators also used autopsy material to look at the rela- tionship between FV Leiden and PE and found no associa- tion ... population. The relationship between PE and DVT As noted by Bounameaux [21], the relationship between FV Leiden, DVT and PE represented a ‘paradox’ in tha...

Ngày tải lên: 12/08/2014, 18:20

... co- incubation with anti-laminin antibody and an antibody against one of the adult MHC isoforms, followed by a fluorescein- tagged secondary antibody against the laminin primary and a rhodamine-tagged ... be significantly altered in the diaphragms of emphysematous animals [1–3,8], although in all reports addressing this is- sue the fibers were typed histochemically rathe...

Ngày tải lên: 12/08/2014, 18:20

... shown. NL-vifS 10 000 10 00 10 0 1 10 0 .1 0. 01 0 2 6 48 Days after infection p24 ng/mL NL-G 116 EvifS 10 000 10 00 10 0 1 10 0 .1 0. 01 0 2 6 48 Days after infection p24 ng/mL NL-4/5SG 116 E6/7S vifS 10 000 10 00 10 0 1 10 0 .1 0. 01 0 2 6 48 Days ... immunodeficiency virus type 1 capsid protein affects viral sensitivity to TRIM5α Ayumu Kuroishi 1...

Ngày tải lên: 13/08/2014, 01:20

... infection, HIV-1 envelope- mediated cell-cell fusion and the infection of HIV-1 captured and transferred by human DCs. Thus, we conclude that GPI-anchored scFv is an effective way to capture transiently-exposed ... Access GPI-anchored single chain Fv - an effective way to capture transiently-exposed neutralization epitopes on HIV-1 envelope...

Ngày tải lên: 13/08/2014, 01:20

... behaved essentially the same as the pseudotypes bearing the T271I mutant Envs in the entry assay (Fig. 3A and 3B), in the syncytial assay, the Δ2N mutant Envs showed a marked reduction in syncytia ... following in vivo replication of mutant virus [87]. These data suggest that the predicted N- link glycosylation sites at N269 and N342 are critical for maintaini...

Ngày tải lên: 13/08/2014, 05:21

... reproduction in any medium, provided the original work is properly cited. Abstract Introduction Postischemic injury to the coronary vascular endothelium, in particular to the endothelial glycocalyx, may provoke ... of coronary vascular leak and interstitial edema and a tendency to forestall both no-reflow and degradation of the endothelial glycocalyx. Introduction...

Ngày tải lên: 13/08/2014, 11:22

... spectrophotometrically at 480 nm. The amount of enzyme that inhibited 50% of epinephrine auto-oxidation was defined as 1 U of SOD activity. The analysis of CAT activity is based on measuring the decrease in hydrogen ... dehydrogenase (type D) into its oxidase (type O) form in hypoxia, followed by the activation of the oxidase form during reoxygenation (normoxia) by the hypoxanth...

Ngày tải lên: 13/08/2014, 13:20

... the meeting ‘The Biology of Genomes’, Cold Spring Harbor, USA, 11-15 May 2005. This year’s Cold Spring Harbor meeting ‘The Biology of Genomes’ presented an eye-opening snapshot of how far the field ... oligonucleotide microarray analysis (ROMA). In this method, a reduced-complexity subset of the genome is selected using a restriction digest and hybridized to microarrays of pro...

Ngày tải lên: 14/08/2014, 14:22