Báo cáo y học: "Analysis of infectious virus clones from two HIV-1 superinfection cases suggests that the primary strains have lower fitnesc" ppt

... as: van der Kuyl et al.: Analysis of infectious virus clones from two HIV-1 superinfection cases suggests that the primary strains have lower fitness. Retrovirology 2010 7:60. Submit your next manuscript ... LTR. However, the LTR from the primary virus B3 from patient P has a lower promoter activity than the LTR from the superinfecting viru...

Ngày tải lên: 13/08/2014, 01:20

... productive infection. Given the importance of this observation, we sought to examine the authenticity of the XMRV integration sites that have been reported to date. Theonlyresearchgroupdescribing ... employed HPLC purified proteins to raise the antisera used for immunostaining, and were unable to find any trace of XMRV at a ll in nearly 800 prostate tumours analysed. They su...

Ngày tải lên: 13/08/2014, 01:20

... infected by an ancestor of the current MeV, the virus probably had different characteristics from the current MeV. Emerging infectious diseases have recently caused sig- nificant morbidity and mortality. ... for RNA viruses including MeV [17,24,25]. Pomeroy et al. also found that the date of divergence of the current MeV was within the last century [17]. In the p...

Ngày tải lên: 12/08/2014, 04:20

... that did not possess similarity to the two dipteran genomes were also analyzed to include ORFs that may be specific to the Aedes genome as well as those that may have diverged significantly from ... clarify the origin of duplicated tran- scripts in the genome assembly. BAC assembly The quality of these BAC assemblies is critical for a valid assessment of the geno...

Ngày tải lên: 14/08/2014, 07:21

... Orthomyxoviri- dae. In Virus Taxonomy, Classification and Nomenclature of Viruses, Eight report of the International Committee on the Taxonomy of Viruses Edited by: Fauquet CM, Mayo MA, Maniloff ... end of the experiment is probably due to high cell density. The viability of mock-infected cells was statistically significant from cells infected with the 4 virus isolat...

Ngày tải lên: 12/08/2014, 04:21

... abolished by substituting the human type residues (Ah), while the very low bind- ing of the human type NS1 (H) is markedl y enhanced by substitution of the avian residues (Ha). This result clearly demon ... determinant. In the majority of human influenza NS1 proteins the consensus motif is RSxV: in avian NS1 it is ESxV. Of the few human strains that have the av...

Ngày tải lên: 11/08/2014, 21:21

... subsequently scored for the appearance of disease symptoms. The infectio n status of the inoculated plants was assessed by visual inspection of symptoms and by PCR analysis of all plants at the end of the ... sequence analyses of the ampli- cons revealed that the majority of the symptomatic plants were infected by begomoviruses belonging to two different spec...

Ngày tải lên: 12/08/2014, 01:22

... charac- terization of the resistance to FV Env-mediated virus entry in these cell lines might ultimately lead to the dis- covery of currently unknown cellular molecules essential for the early stages of FV ... with respect to their genome structure. The FV replication strategy deviates in many aspects from that of orthoretro- viruses [reviewed in [1]]. Interestingly, man...

Ngày tải lên: 12/08/2014, 23:23

... results, expression of Vif from pNL-A1 only moderately reduced the steady-state levels of APOBEC3G in HeLa cells. Quan- titation of the data confirmed that expression of APOBEC3G in the presence of Vif was ... the activity of the accessory protein Vif. One of the prerequi- sites for the antiviral activity of APOBEC3G is that it is Effect of fixation metho...

Ngày tải lên: 13/08/2014, 13:20

... appears that the two clusters of mutations may affect two different functional domains required for proteinase activity. Findings Vaccinia virus (VV) is the prototypic member of the orthopoxviruses, ... by disrupting the arrangement of the catalytic triad due to its proximity to the cysteine resi- due at aa328. It is possible that the other mutants, with amino ac...

Ngày tải lên: 20/06/2014, 01:20