Báo cáo y học: " Expression of Nef from unintegrated HIV-1 DNA downregulates cell surface CXCR4 and CCR5 on T-lymphocytes" docx

... assayed by flow cytometry for cell surface expression of CD4, CXCR4 and CCR5 (Figure 2). Potent downregulation of CD4 by Nef was shown to occur, with cell surface levels being only ≈5% of those ... original work is properly cited. Research Expression of Nef from unintegrated HIV-1 DNA downregulates cell surface CXCR4 and CCR5 on T-lymphocyt...

Ngày tải lên: 12/08/2014, 23:23

... contains only one PAPP-A gene. Thus, PAPP-A and PAPP-Ai mRNA likely results from alternative splicing of a transcript from the same gene. Expression and functional analysis of murine PAPP-A and ... between human and murine PAPP-A. Our cloning of cDNA encoding both PAPP-A and PAPP-Ai allowed expression in mammalian cells and functional analyses of the recombinant pr...

Ngày tải lên: 31/03/2014, 21:21

... inactivation study Genomic DNA was extracted from 10 ml of peripheral blood lymphocyte of patients and controls using standard methods [16]. Genotyping of a polymorphic site in the androgen recep- tor ... age-matched healthy women (n = 257). XCI analysis was performed by enzymatic digestion of DNA with a methylation sensitive enzyme (HpaII) followed by PCR of a polymorphic C...

Ngày tải lên: 09/08/2014, 14:22

... study. By contrast, in the COPE study patients only return ed to their usual medication following an examination and decision by one of the trial physicians. Thus, patients in WISP and COSMIC may ... nly able to conduct one meta-analysis. Meta-analysis There are reasons for being cautious about the one meta-analysis we conducted and, in general, meta-ana- lysing results from these ty...

Ngày tải lên: 12/08/2014, 13:22

... gene expression in adipocytes. It may be also speculated that the effect of adrenergic system on mitochondrial biogenesis represents a compensation for decreased ef®ciency of energy conversion in ... insuf®cient supply o f ATP by mitochon- drial oxidative phosphorylation [20]. Besides UCP1, ef®ciency of oxidative phosphorylation in adipocytes may b e also c ontrolled b y r ecently...

Ngày tải lên: 31/03/2014, 15:20

... study the contribution of monocytes overexpressing TLR-2 to the lung inflammation of COPD patients and also the possible effect of lung environment on the expression of TLR-2 after monocytes ... produced by cells undergoing stress or necrosis [30,31]. Considering that COPD is characterized by considerable tissue injury Effect of methylprednisolone on the expression of TL...

Ngày tải lên: 12/08/2014, 16:20

... supernatant and inclusion body. Part of the production was applied to 12% SDS–PAGE analysis and Western blot analysis. (A) SDS-PAGE analysis: Most of the fusion protein was found in inclusion body. The ... treatment of type 2 diabetes [2]. Glucagon-like peptide 1 (GLP-1) is an incretin hormone released from islet  -cell and intestinal L-cells in response to the ingestion...

Ngày tải lên: 25/10/2012, 11:10

... Negative controls consisted of omission of the primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit serum instead of the primary antibody. Cell quantification ... is activated and incises the newly synthesized DNA strand that contains the mutation [14]. Immunohistochemical study on non-polyposis colorectal cancer demonstrated no e...

Ngày tải lên: 03/11/2012, 09:54

... hybridization. The nucleotide sequences of plasmid DNAs were confirmed by restriction mapping and by DNA sequencing. In the cases of AACEVAPD3 and 6, one TAA codon in their ORFs, thus should be converted ... [10]. The YN11 strain was derived from YPH499 strain, and YN45 strain was prepared by the use of YN11 and BJ5458 strains. Preparation of AACEVAPD1 –6 5 0 RACE and 3 0...

Ngày tải lên: 08/03/2014, 23:20

... a comparative study in mice of UCP3 protein expression in SS and IMF mitochondria of various muscle types. We also examined UCP3 protein expression in the two mitochon- dria populations of fed and fasted ... regulation of UCP3 protein expres- sion may have important implications for the understanding of its physiological role. In conclusion, in this study, using a carefully...

Ngày tải lên: 24/03/2014, 04:21