Báo cáo y học: " Encapsidation of APOBEC3G into HIV-1 virions involves lipid raft association and does not correlate with APOBEC3G oligomerization" potx

... purposes) Retrovirology Open Access Research Encapsidation of APOBEC3G into HIV-1 virions involves lipid raft association and does not correlate with APOBEC3G oligomerization Mohammad A Khan, Ritu Goila-Gaur, Sandra ... between encapsidation, lipid raft association, and genomic RNA binding of A3G. Surprisingly, the presence of a C-terminal epitope...

Ngày tải lên: 12/08/2014, 23:22

... immunolocalization of TSP-2 in the human disc and the normal mouse disc, and sub- sequently examined mice with a targeted disruption of the TSP-2 gene with respect to the morphology and cellularity of the ... discsMorphology of collagen lamellae in the annulus of a mouse discs. The morphology was examined with Masson-trichrome stain and with polar- ized light micros...

Ngày tải lên: 09/08/2014, 10:23

... immunodeficiency virus type 1 (HIV-1) -based gene delivery systems are popular due to their superior efficiency of transduction of primary cells. However, these systems cannot be readily used for delivery of ... Research and Therapy Open Access Research Substitution of the Rev-response element in an HIV-1- based gene delivery system with that of SIVmac239 allows efficient deli...

Ngày tải lên: 10/08/2014, 05:21

... page) (a) (b) (c) (d) YBL001C 4.2E-04 YBL044W 1.5E-06 YCL065W 8.0E-05 YCL066W 8.0E-05 YCL067C 8.0E-05 YCR039C 4.7E-05 YCR040W 4.7E-05 YCR041W 4.7E-05 YCR105W 1.7E-04 YDL066W 1.4E-08 YDR299W 1.3E-05 YDR473C ... 1.5E-05 YDR543C 4.8E-05 YDR544C 4.5E-04 YDR545W 4.5E-04 YEL025C 4.7E-10 YGL001C 3.5E-06 YGR296W 2.0E-04 YHR001W 2.5E-04 YHR193C 4.1E-05 YJR079W 1.4E-09 YKL015W 2.9E-05 YLR169W 2.7E-04 Y...

Ngày tải lên: 14/08/2014, 17:22

... Group, 2 Anatomy and 3 Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada The Na + /H + exchanger is a ubiquitous membrane protein of bacteria, plants and ... microdomains. Treatment of cells with methyl b-cyclodextrin had a small stimulatory effect on Na + /H + exchanger activity and reduced the amount of Na + /H + exchanger i...

Ngày tải lên: 08/03/2014, 16:20

... (both 10 ng/ml). Cytokine analysis IFN-γ and IL-4 production were analyzed by ELISA upon costimulation with CD3/CD28 and cytokines after 5 days of culture. After both 5 and 10 days of culture, cells ... the absence of or in the presence of IL-7 and/ or IL-4. The production of IFN-γ and IL-4 was measured by ELISA and by single-cell FACS analysis to indicate Th1 and Th2...

Ngày tải lên: 09/08/2014, 01:23

... pathways and identify susceptibility genes or susceptibility effects that might be epistatic, additive or independent. Similarly, the psoriasis study analyzed a region previously identified by linkage ... transfection experiments with which they could show the allelic effect of the polymorphisms on gene expression in reporter assays and their effect by co- transfection of RUNX1. In...

Ngày tải lên: 09/08/2014, 01:23

... two patients with SS [6]. Detailed analyses of the frequency of occurrence and mutations of the highly mutable motifs RGYW and WRCY revealed that mutations in nonproductive V L rearrange- ments of B ... nonproductive and productive repertoires of these donors exhibited a comparable usage of Vκ and Jκ as well as Vλ and Jλ gene elements [10,11]. Determination of Taq po...

Ngày tải lên: 09/08/2014, 03:24

... conception and design of this study, carried out the cellular, molecular, and genetic studies, and drafted the manuscript. YWS was involved in the conception and design of this study and helped draft ... was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assays and by flow cytometric analysis after propidium iodide (PI) staining. Bcl-2 expressi...

Ngày tải lên: 09/08/2014, 14:22

... Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta 1986, 883:173-177. 27. Le Maitre CL, Freemont AJ, Hoyland JA: Accelerated ... quantification of DNA and glycosaminogly- cans (GAG) content using the pico green assay (Invitrogen, Paisley, UK) and the dimethylmethylene blue (DMMB) assay. The beads were sol...

Ngày tải lên: 09/08/2014, 14:22