Báo cáo y học: " Human APOBEC1 cytidine deaminase edits HBV DNA Minerva Cervantes Gonzalez, Rodolphe Suspène, Michel Henry, Denise Guétard, Simon Wain-Hobson and " pot

... cytoplasm and nucleus, it is potentially a more likely pro-cancerous candidate than hA3G, which is strictly cytoplasmic. Human APOBEC1 can efficiently hyperedit HBV minus strand DNAFigure 1 Human APOBEC1 ... in newly synthesized HIV-1 DNA. Nature 2003, 424:94-98. 28. Henry M, Guetard D, Suspene R, Rusniok C, Wain-Hobson S, Varta- nian JP: Genetic editing of HBV DNA by monod...

Ngày tải lên: 12/08/2014, 23:22

... he study. Patients had no history of treatment for HBV prior to the study and they also had no alcohol consumption. Study protocol was approved by the Ethics Committee of Tehran University of Medical ... increasing HDL and adipo- nectin variables decrease Log HBV DNA. TNF-a was significantly elevated in patient s with HBV DNA levels more than med ian compared wit h those in HBV...

Ngày tải lên: 12/08/2014, 01:21

... they are the only animals that pass oocysts in their feces. They become infected by eating infected rodents, birds, or other small animals. Once oocysts are shed, they re- quire 1 to 5 days ... it is not necessary, for example, to give away one’s cat during pregnancy and that if they follow a few very simple “rules”, liv- ing with their pets need not represent any risk for infection .....

Ngày tải lên: 03/11/2012, 11:06

... m M phenylmethanesulfonyl¯uoride], and the samples were fractionated b y electrophoresis on a 12% SDS/polyacrylamide gel. The separated polypeptides were electotransferred to a poly(vinylydene ... with genomic DNA data (accession numbers O76865 and O9W4W6) and i ndicated by the arrowheads. The s ymbol ÔVÕ indicates a po ten tial N - glycosylation site. A putative polyadenylation sig...

Ngày tải lên: 21/02/2014, 15:20

... Isotype specificity analysis of the Z IgA1 affibody. Resulting overlay sensorgrams from separate injections of 0.2 l M solutions of human polyclonal IgA and IgA 1 ,humanmyelomaIgA 2 and IgM, human ... micromolar affinity of the interaction was sufficient for affinity chromatography and that the selectivity of the ligand was high. Affinity recovery of IgA from human plasma The potential u...

Ngày tải lên: 08/03/2014, 23:20

... etected in human embryonic and fetal pancreas and in pancreatic tumoral cell lines [35,36]. FAPP and BSSL are structurally closely related, but are distinguished by a monoclonal antibody directed ... Carboxyl ester lipase: a highly polymorphic locus on human chromosome 9qter. Ge nomics 10, 425±431. 24. Hui, D .Y. (1996) Molecular biology of enzymes involved with cholesterol ester hy...

Ngày tải lên: 24/03/2014, 03:21

... previously been reported to bind nonspecifically to nucleic acids [21]. SF2/ ASF could thus preferentially bind to DNA and prevent this way DNA from cutting by htopo I. We excluded this possibility showing ... SF2/ASFÕ. The amount of htopo I and SF2/ASF was quantified by densitometric scanning of SDS-polyacrylamide gels using BSA as a standard. DNA cleavage assay Routinely, the DNA...

Ngày tải lên: 24/03/2014, 03:21

... two Alu-repeats and three CAGAC motifs [24]. BLAST searches revealed the homology of the coding sequences of the 4.9- and 7-kb cDNAs with the sprouty (spry) gene family. Homology with murine spry4 (mspry4) was ... co-immunopre- cipitated Myc-TESK1. Conversely, anti-Myc Ig precipitated the HA-tagged hspry4 protein from COS cells, that were transfected with both Myc-TESK1 cDNA and HA-hspr...

Ngày tải lên: 31/03/2014, 15:20

... University of Bologna, Italy, 2 Faculty of Veterinary Medicine, University of Bologna, Italy, 3 Faculty of Human Sciences, LUdeS University, Lugano, Switzerland and 4 Department of Physiology and ... regulatory and catalytic subunits. Biol Psychiatry 2004, 55:234-243. 25. Pandey GN, Dwivedi Y, Ren X, Rizavi HS, Mondal AC, Shukla PK, Conley RR: Brain region specific alterations...

Ngày tải lên: 08/08/2014, 23:21

... exclusively in patients with polymyositis, a syndrome characterized by chronic muscle inflammation of unknown cause [12-14], and they seem to define a distinct phenotypic, genetic and epide- miologic ... 5–15 is from a polymyositis patient without detect- able myositis autoantibodies, and serum 1–24 is from a polymyositis patient with autoantibodies directed against histidyl-tRNA synthe...

Ngày tải lên: 09/08/2014, 07:20