Báo cáo y học: " Mechanism of HIV-1 Tat RNA translation and its activation by the Tat protein" ppsx

... cloning of the Tat mRNA sequences To study the mechanism of protein synthesis from the Tat mRNA, we cloned the Tat1 and Tat2 sequences, the two major forms of Tat mRNA [43], by means of a PCR-DNA reconstruction ... of 18 (page number not for citation purposes) Retrovirology Open Access Research Mechanism of HIV-1 Tat RNA translation and its a...

Ngày tải lên: 12/08/2014, 23:22

... to the binding of the metal to the N-lobe, although the binding favorsthe C-lobe thermodynamically as judged by 1 H NMR. The more open conformation of the N- lobe may facilitate Yb 3+ ion binding ... iron requirement and subsequently express much higher Tf receptors [39]. Then the uptake of Yb-bound Tf by the tumor cells might be much higher than the normal cells...

Ngày tải lên: 08/03/2014, 09:20

... bifunctional ligand, has been neglected. The consistency of the K d values resulting from the evaluation of the equilibrium response signals and those obtained by evaluating the first step in the association ... previously [13,35]. The K d determined for factor X is consistent with the value determined by McDonald et al.[9]. The effect of the serine protease dom...

Ngày tải lên: 08/03/2014, 23:20

... diag- nosis of infection with human immunodeficiency virus type 1 (HIV-1) by detection of antibody IgGs to pol and gag proteins of HIV-1 and p24 antigen of HIV-1 in urine, saliva, and/ or serum ... an indicator of subject's state of health[26]. HIV-1 RNA/ DNA and CD4 mRNA detected in the fecal specimens of HIV-1 infected subjects could be the result e...

Ngày tải lên: 10/08/2014, 05:21

... when the ALT level was maximum. Clinical parameter and pregnancy The maximum duration of viremia was 42 days and 36 days, fecal shedding 28 days and 19 days and IgM anti HEV 46 days and 49 days ... from the study because of lack of desired follow up. The mean age of the patients was 29.2 ± 8.92 years (range: 11 to 54 years) and there were 39 males and 21 female...

Ngày tải lên: 12/08/2014, 01:21

... normal function of AEC by a variety of mechanisms. Some of these include decreases in the level of exhaled NO, enhanced release of pro-inflammatory cytokines, and inhibition of the airway repair process ... prostaglandins and a variety of cytokines [7]. In the lungs and skin of smokers mast cells increase in absolute numbers and smoking may be associated with...

Ngày tải lên: 12/08/2014, 18:22

... lymphocytes and monocytes. We have previously shown that IκB-αS32/36A, a proteolysis-resistant inhibitor of NF-κB, potently inhibits the growth of HIV-1 and SIVmac239 in cell cultures and in the ... vivo. Background HIV-1 infection is characterized by a long period of clini- cal latency followed by the development of acquired immunodeficiency syndrome, AIDS. During...

Ngày tải lên: 13/08/2014, 13:20

... isoforms of LIF mRNA. Expression of LIF by myofibroblasts and of its receptor by adjacent cells suggests a potential LIF paracrine loop in human liver that may play a role in the regulation of ... of endothelial cell and/ or hepatocyte labeling. Immunohistochemistry, flow cytometry and STAT-3 phosphorylation assays did not provide evidence for LIF receptor expression b...

Ngày tải lên: 13/08/2014, 13:20

... homozygous and TIRAP/Mal heterozygous. Of 29 TLR4 heterozygous mutation carriers, 24 were TIRAP/Mal heterozygous and 5 homozygous. The distribution of SNPs in the studied patients and the cohort of ... JMWvdM and KZ contributed to conception and design of the study. Statistical analysis was performed by OK and EJG-B. RRS headed the project, supervised and con...

Ngày tải lên: 13/08/2014, 20:22

... phenol-chloroform extraction steps as prescribed by the manufacturer. Microarray analysis of gene-expression profiles Microarrays were generated by spotting the Compugen 19 K human oligo library, made by ... respectively, and hybridized to the arrays using protocols established by the Patrick O. Brown Laboratory [59]. Array images and data were obtained and processed u...

Ngày tải lên: 14/08/2014, 14:21