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Báo cáo y học: " Proviral integrations and expression of endogenous Avian leucosis virus during long term selection for high and low body weight in two chicken lines" ppt

Báo cáo y học:

Báo cáo y học: " Proviral integrations and expression of endogenous Avian leucosis virus during long term selection for high and low body weight in two chicken lines" ppt

... AccessResearch Proviral integrations and expression of endogenous Avian leucosis virus during long term selection for high and low body weight in two chicken linesSojeong Ka1, Susanne Kerje1,2,4,5, Lina Bornold1, ... anadvanced intercross of the lines. Correlation analysis between expression, proviral integrations and body weight showed that high ALVE levels in the LWS line were inherited and that more ALVE integrations ... of crosses among seven lines of White Plymouth Rock chick-ens has generated two extremely divergent lines; the low (LWS) and high weight selection (HWS) lines. The aver-age body weight of individuals...
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Báo cáo y học: " Restriction by APOBEC3 proteins of endogenous retroviruses with an extracellular life cycle: ex vivo effects and in vivo "traces" on the murine IAPE and human HERV-K elements" pot

... effects against a vari-ety of viruses, including numerous retroviruses – i.e. HIV,SIV, MLV, HTLV and foamy viruses –, hepatitis B virus and adeno-associated virus (AAV) (for review [11]). In con-trast ... whichaccount for ~30% of each mammalian genome, and theLTR-containing retroelements (including the endogenous retroviruses, ERVs), accounting for ~10% of the genomes and closely related to retroviruses. ... pro-teins might have actually impaired the in vivo amplifica-tion of these elements in the past, by searching for evidence of APOBEC3-editing on the endogenous copiesresiding in the murine and...
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Báo cáo y học: "Gender-based reciprocal expression of transforming growth factor-β1 and the inducible nitric oxide synthase in a rat model of cyclophosphamide-induced cystitis" ppsx

... following tissue injury in the upper urinarytract. Since the role of TGF-β1 in lower urinary tract inflammation is currently unknown, and sincegender-based differences exist in the setting of ... void-ing and histology characteristic of cyclophosphamide(CYP)-induced cystitis in rats [4]. Temporal changes in thelevels of pro-inflammatory cytokines and chemokinessuch as interleukin IL-1α, ... reflect the state of theacrolein-injured bladder. Given the interplay of regulatoryinfluences operating in the production of NO, TGF-β1 and other pro-inflammatory cytokines in bladder inflam-mation,...
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Báo cáo khoa học: Stage-specific expression of Caenorhabditis elegans ribonuclease H1 enzymes with different substrate specificities and bivalent cation requirements ppt

Báo cáo khoa học: Stage-specific expression of Caenorhabditis elegans ribonuclease H1 enzymes with different substrate specificities and bivalent cation requirements ppt

... unit of enzymatic activity was defined asthe amount of enzyme hydrolyzing 1 lmol substrate perminute, and the specific activity was defined as the enzy-matic activity per mg protein. Enzymatic ... identified by using hmmpfam[41], and we extracted the amino-acid sequences corres-ponding to the RNase H domain. fasta analysis [42] wasperformed to analyze protein similarity by comparing theamino-acid ... ‘Understanding and Control of Life’s Function via Systems Biology(Keio University)’; and grants from the Japan Society for the Promotion of Science (JSPS) and Keio Univer-sity.References1 Stein...
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Báo cáo y học: "Catabolic stress induces expression of hypoxia-inducible factor (HIF)-1α in articular chondrocytes: involvement of HIF-1α in the pathogenesis of osteoarthritis" ppt

... through a combination of enzymology and immunohistochemistry techniques. Biotinylated nucle-otides are incorporated into the 3'-OH ends of the DNA frag-ments by terminal deoxynucleotidyl transferase ... solution and then decalcified in 4% paraformaldehyde containing 0.85% sodium chloride and 10% acetic acid. Tissues were dehydrated in a series of ethanol solutions and infiltrated with xylene and before ... by proline hydroxylation: implications for O2sensing. Science 2001, 292:464-468.29. Huang J, Zhao Q, Mooney SM, Lee FS: Sequence determinants in hypoxia-inducible factor-1alpha for hydroxylation...
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... L-methionine, degrading cysthationine via homocysteine.L-valine and L-isoleucineThe terminal four steps in the biosynthesis of L-valine and L-isoleucine employ a common set of widely distributedenzymes, ... domains of life, and eight of their branches probably occurred in the LCAL-arginineThere are at least four L-arginine synthesis pathways, inter-playing with the conversion of L-ornithine and ... analyzedhere. Interestingly, both L-lysine biosynthetic superpathwaysretain groups of duplicated genes for the biosynthesis of L-leucine and L-arginine (Figure 3), which, as detailed above,probably occurred...
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Báo cáo y học: " Intragenic transcriptional cis-activation of the human immunodeficiency virus 1 does not result in allele-specific inhibition of the endogenous gene" pdf

... μg/ml of GST-Tat toinduce LTR expression as described previously [34,47].The following day cells were incubated in hypoxanthine,aminopterin and thymidine (HAT) medium and thencloned by colony ... obtained by limiting dilutions and colonies were vis-ually scored for low basal level of ECFP expression in thecytoplasm and to be highly inducible by GST-Tat by fluo-rescence microscopy. The ... picking and expansion in completeDMEM medium. Colonies were visually scored for low basal level of ECFP expression and to be highly inducibleby GST-Tat by fluorescence microscopy. The number of integrations...
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Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx

Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx

... usuallyprecedes the guanine nucleotide-binding NKXD motif byalanine and replacement of a conserved phenylalanine 10residues C-terminal to the EXS[AV] motif by alanine,threonine, serine or ... showing 10 lg of platelet protein and 0.5 lg of GST and GST±Rab p roteins; (B) an[a-32P]GTP overlay [17] of a nitrocellulose blot of a gel containing40 lg of platelet protein, 0.5 lgofGSTand0.5lgofGST±Rabproteins, ... were determined in 180±240 min incubations at 37 °C in the presence of 5 lM[c-32P]GTP and the indicatedconcentrations of Mg2+free, and are expressed as nmol of GTP hydrolysedámg protein)1ámin)1....
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Báo cáo Y học: The cold-active lipase of Pseudomonas fragi Heterologous expression, biochemical characterization and molecular modeling pdf

Báo cáo Y học: The cold-active lipase of Pseudomonas fragi Heterologous expression, biochemical characterization and molecular modeling pdf

... peptide-independent pathway and calls for furtherinvestigation on this subject.Enzyme activity and specificity In the standard tricaprylin hydrolysis assay, rPFL displayedhighest activity at 29 ... number of disulphide bridges, the location of proline and arginine residues, and hydrophobic coreinteractions are generally recognized as key factors in determining the flexibility of the enzyme ... presence of disulphide bridges mayincrease the rigidity of the protein backbone and thereforeits thermostability [35]. Interestingly, the only disulphidebridge present in BCL and PAL is missing in...
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Báo cáo y học: " HIV-1 transgene expression in rats causes oxidant stress and alveolar epithelial barrier dysfunction" doc

... Winchester RJ, Ginsberg HS: Human immu-nodeficiency virus type 1 strains in the lungs of infected indi-viduals evolve independently from those in peripheral blood and are highly conserved in ... cells, and lung fluid(necessary for the findings in all of the figures), and wrotethe initial draft of the manuscript. XF determined tightjunction (occludin and ZO-1) gene and protein expres-sion ... pro-teins in vivo.Discussion In this study we determined that HIV-1 transgene expres-sion in rats, in which HIV-related proteins includinggp120 and Tat are expressed in the absence of viral infec-tion...
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