... 288–300.21 Tsutsui N, Saido-Sakanaka H, Yang WJ, Jayasankar V,Jasmani S, Okuno A, Ohira T, Okumura T, Aida K &Wilder MN (2004) Molecular characterization of a cDNA encoding vitellogenin ... primers(forward, 5¢-TAATACGACTCACTATAGGTACTATGTATCGCATGCCAAT-3¢; reverse, 5¢-TAATACGACTCACTATAGGTACTTTAAAGTCCCGGGTTGA-3¢). ForPCR, the reaction mix consisted of 1 · Taq buffer contain-ing 1.5 mm MgCl2, ... RNAiTo prepare a DNA template for the synthesis of dsRNA,DNA corresponding to the mature peptide of MeMIH-Bwas amplified by PCR using T7 promoter-linked primers(forward, 5¢-TAATACGACTCACTATAGGTACTATGTATCGCATGCCAAT-3¢;...